Signals of seminal vesicle autoantigen suppresses bovine serum albumin-induced capacitation in mouse sperm

Yen Hua Huang, Shin Peih Kuo, Mei Hsiang Lin, Chwen Ming Shih, Sin Tak Chu, Chih Chun Wei, Tasi Jung Wu, Yee Hsiung Chen

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Capacitation is the prerequisite process for sperm to gain the ability for successful fertilization. Unregulated capacitation will cause sperm to undergo a spontaneous acrosome reaction and then fail to fertilize an egg. Seminal plasma is thought to have the ability to suppress sperm capacitation. However, the mechanisms by which seminal proteins suppress capacitation have not been well understood. Recently, we demonstrated that a major seminal vesicle secretory protein, seminal vesicle autoantigen (SVA), is able to suppress bovine serum albumin (BSA)-induced mouse sperm capacitation. To further identify the mechanism of SVA action, we determine the molecular events associated with SVA suppression of BSA's activity. In this communication, we demonstrate that SVA suppresses the BSA-induced increase of intracellular calcium concentration ([Ca 2+] i), intracellular pH (pH i), the cAMP level, PKA activity, protein tyrosine phosphorylation, and capacitation in mouse sperm. Besides, we also found that the suppression ability of SVA against BSA-induced protein tyrosine phosphorylation and capacitation could be reversed by dbcAMP (a cAMP agonist).

Original languageEnglish
Pages (from-to)1564-1571
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume338
Issue number3
DOIs
Publication statusPublished - Dec 23 2005

Fingerprint

Bovine Serum Albumin
Spermatozoa
Sperm Capacitation
Phosphorylation
Tyrosine
Seminal Vesicle Secretory Proteins
Acrosome Reaction
Proteins
Semen
Fertilization
Ovum
seminal vesicle autoantigen
Calcium
Plasmas
Communication

Keywords

  • Ca
  • cAMP-PKA pathway
  • Capacitation
  • Protein tyrosine phosphorylation
  • Seminal vesicle

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Signals of seminal vesicle autoantigen suppresses bovine serum albumin-induced capacitation in mouse sperm. / Huang, Yen Hua; Kuo, Shin Peih; Lin, Mei Hsiang; Shih, Chwen Ming; Chu, Sin Tak; Wei, Chih Chun; Wu, Tasi Jung; Chen, Yee Hsiung.

In: Biochemical and Biophysical Research Communications, Vol. 338, No. 3, 23.12.2005, p. 1564-1571.

Research output: Contribution to journalArticle

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