Signaling pathways for induction of platelet aggregation by sas tongue cancer cells - A mechanism of hematogenous metastasis

Mei Chi Chang, Chiu Po Chan, Yuan Soon Ho, Jang Jaer Lee, Po Shuen Lin, Bor Ru Lin, Ya Ling Huang, Liang Jiunn Hahn, Hung Wei Yeh, Ying Jan Wang, Jiiang Huei Jeng

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background: Tongue cancer metastasis is mainly through blood stream and possibly associated with tumor cell-induced platelet aggregation (TCIPA). Methods: Platelet aggregation was induced by different amounts of SAS tongue cancer cells with/without inhibitors and the latent period for induction of platelet aggregation was recorded. Gene expression was analyzed by reverse transcriptase-polymerase chain reaction. Results: SAS cells (4 × 10 4 to 1 × 10 6 cells/ml) induced platelet aggregation in a cell density-dependent manner. The latent period for induction of platelet aggregation reduced from 11.3 min (2 × 10 5 cells/ml) to 0.9 min (5 × 10 5 cells/ml). The extent of platelet aggregation increased from 39% to 76% by 2 × 10 5 and 5 × 10 5 SAS cells. Pre-treatment of SAS cells with aspirin showed little effect on its induction of platelet aggregation. SAS cells expressed tissue factor (TF) mRNA and the SAS cells-induced TCIPA was inhibited by TF neutralization antibody (5-20 μg/ml), heparin (5-10 U/ml), Hirudin fragment 54-65 (50 μg/ml) and D-Phenylalanyl-L-prolyl-L-arginine chloromethyl ketone. But areca nut (AN, a betel quid component known to generate reactive oxygen species (ROS)) extract showed little effect on TF expression in SAS cells. Pre-treatment with U73122 and 2-aminoethoxydiphenylborate inhibited SAS-induced TCIPA. Interestingly, catalase suppressed SAS cells-induced TCIPA, whereas AN extract enhanced this event. Conclusions: These results suggest that tongue cancer cells may induce TCIPA and enhance tumor metastasis. SAS-induced TCIPA is related to TF secretion, thrombin generation and associated with Phospholipase C-Inositol triphosphate signaling and ROS production. Betel quid chewing may potentially promote tongue cancer metastasis.

Original languageEnglish
Pages (from-to)434-440
Number of pages7
JournalJournal of Oral Pathology and Medicine
Volume38
Issue number5
DOIs
Publication statusPublished - May 2009

Fingerprint

Tongue Neoplasms
Platelet Aggregation
Neoplasm Metastasis
Thromboplastin
Neoplasms
Reactive Oxygen Species
Areca

Keywords

  • Areca nut
  • Reactive oxygen species
  • Tissue factor
  • Tumor cells-induced platelet aggregation
  • Tumor metastasis

ASJC Scopus subject areas

  • Cancer Research
  • Pathology and Forensic Medicine
  • Otorhinolaryngology
  • Oral Surgery
  • Periodontics

Cite this

Signaling pathways for induction of platelet aggregation by sas tongue cancer cells - A mechanism of hematogenous metastasis. / Chang, Mei Chi; Chan, Chiu Po; Ho, Yuan Soon; Lee, Jang Jaer; Lin, Po Shuen; Lin, Bor Ru; Huang, Ya Ling; Hahn, Liang Jiunn; Yeh, Hung Wei; Wang, Ying Jan; Jeng, Jiiang Huei.

In: Journal of Oral Pathology and Medicine, Vol. 38, No. 5, 05.2009, p. 434-440.

Research output: Contribution to journalArticle

Chang, Mei Chi ; Chan, Chiu Po ; Ho, Yuan Soon ; Lee, Jang Jaer ; Lin, Po Shuen ; Lin, Bor Ru ; Huang, Ya Ling ; Hahn, Liang Jiunn ; Yeh, Hung Wei ; Wang, Ying Jan ; Jeng, Jiiang Huei. / Signaling pathways for induction of platelet aggregation by sas tongue cancer cells - A mechanism of hematogenous metastasis. In: Journal of Oral Pathology and Medicine. 2009 ; Vol. 38, No. 5. pp. 434-440.
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abstract = "Background: Tongue cancer metastasis is mainly through blood stream and possibly associated with tumor cell-induced platelet aggregation (TCIPA). Methods: Platelet aggregation was induced by different amounts of SAS tongue cancer cells with/without inhibitors and the latent period for induction of platelet aggregation was recorded. Gene expression was analyzed by reverse transcriptase-polymerase chain reaction. Results: SAS cells (4 × 10 4 to 1 × 10 6 cells/ml) induced platelet aggregation in a cell density-dependent manner. The latent period for induction of platelet aggregation reduced from 11.3 min (2 × 10 5 cells/ml) to 0.9 min (5 × 10 5 cells/ml). The extent of platelet aggregation increased from 39{\%} to 76{\%} by 2 × 10 5 and 5 × 10 5 SAS cells. Pre-treatment of SAS cells with aspirin showed little effect on its induction of platelet aggregation. SAS cells expressed tissue factor (TF) mRNA and the SAS cells-induced TCIPA was inhibited by TF neutralization antibody (5-20 μg/ml), heparin (5-10 U/ml), Hirudin fragment 54-65 (50 μg/ml) and D-Phenylalanyl-L-prolyl-L-arginine chloromethyl ketone. But areca nut (AN, a betel quid component known to generate reactive oxygen species (ROS)) extract showed little effect on TF expression in SAS cells. Pre-treatment with U73122 and 2-aminoethoxydiphenylborate inhibited SAS-induced TCIPA. Interestingly, catalase suppressed SAS cells-induced TCIPA, whereas AN extract enhanced this event. Conclusions: These results suggest that tongue cancer cells may induce TCIPA and enhance tumor metastasis. SAS-induced TCIPA is related to TF secretion, thrombin generation and associated with Phospholipase C-Inositol triphosphate signaling and ROS production. Betel quid chewing may potentially promote tongue cancer metastasis.",
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T1 - Signaling pathways for induction of platelet aggregation by sas tongue cancer cells - A mechanism of hematogenous metastasis

AU - Chang, Mei Chi

AU - Chan, Chiu Po

AU - Ho, Yuan Soon

AU - Lee, Jang Jaer

AU - Lin, Po Shuen

AU - Lin, Bor Ru

AU - Huang, Ya Ling

AU - Hahn, Liang Jiunn

AU - Yeh, Hung Wei

AU - Wang, Ying Jan

AU - Jeng, Jiiang Huei

PY - 2009/5

Y1 - 2009/5

N2 - Background: Tongue cancer metastasis is mainly through blood stream and possibly associated with tumor cell-induced platelet aggregation (TCIPA). Methods: Platelet aggregation was induced by different amounts of SAS tongue cancer cells with/without inhibitors and the latent period for induction of platelet aggregation was recorded. Gene expression was analyzed by reverse transcriptase-polymerase chain reaction. Results: SAS cells (4 × 10 4 to 1 × 10 6 cells/ml) induced platelet aggregation in a cell density-dependent manner. The latent period for induction of platelet aggregation reduced from 11.3 min (2 × 10 5 cells/ml) to 0.9 min (5 × 10 5 cells/ml). The extent of platelet aggregation increased from 39% to 76% by 2 × 10 5 and 5 × 10 5 SAS cells. Pre-treatment of SAS cells with aspirin showed little effect on its induction of platelet aggregation. SAS cells expressed tissue factor (TF) mRNA and the SAS cells-induced TCIPA was inhibited by TF neutralization antibody (5-20 μg/ml), heparin (5-10 U/ml), Hirudin fragment 54-65 (50 μg/ml) and D-Phenylalanyl-L-prolyl-L-arginine chloromethyl ketone. But areca nut (AN, a betel quid component known to generate reactive oxygen species (ROS)) extract showed little effect on TF expression in SAS cells. Pre-treatment with U73122 and 2-aminoethoxydiphenylborate inhibited SAS-induced TCIPA. Interestingly, catalase suppressed SAS cells-induced TCIPA, whereas AN extract enhanced this event. Conclusions: These results suggest that tongue cancer cells may induce TCIPA and enhance tumor metastasis. SAS-induced TCIPA is related to TF secretion, thrombin generation and associated with Phospholipase C-Inositol triphosphate signaling and ROS production. Betel quid chewing may potentially promote tongue cancer metastasis.

AB - Background: Tongue cancer metastasis is mainly through blood stream and possibly associated with tumor cell-induced platelet aggregation (TCIPA). Methods: Platelet aggregation was induced by different amounts of SAS tongue cancer cells with/without inhibitors and the latent period for induction of platelet aggregation was recorded. Gene expression was analyzed by reverse transcriptase-polymerase chain reaction. Results: SAS cells (4 × 10 4 to 1 × 10 6 cells/ml) induced platelet aggregation in a cell density-dependent manner. The latent period for induction of platelet aggregation reduced from 11.3 min (2 × 10 5 cells/ml) to 0.9 min (5 × 10 5 cells/ml). The extent of platelet aggregation increased from 39% to 76% by 2 × 10 5 and 5 × 10 5 SAS cells. Pre-treatment of SAS cells with aspirin showed little effect on its induction of platelet aggregation. SAS cells expressed tissue factor (TF) mRNA and the SAS cells-induced TCIPA was inhibited by TF neutralization antibody (5-20 μg/ml), heparin (5-10 U/ml), Hirudin fragment 54-65 (50 μg/ml) and D-Phenylalanyl-L-prolyl-L-arginine chloromethyl ketone. But areca nut (AN, a betel quid component known to generate reactive oxygen species (ROS)) extract showed little effect on TF expression in SAS cells. Pre-treatment with U73122 and 2-aminoethoxydiphenylborate inhibited SAS-induced TCIPA. Interestingly, catalase suppressed SAS cells-induced TCIPA, whereas AN extract enhanced this event. Conclusions: These results suggest that tongue cancer cells may induce TCIPA and enhance tumor metastasis. SAS-induced TCIPA is related to TF secretion, thrombin generation and associated with Phospholipase C-Inositol triphosphate signaling and ROS production. Betel quid chewing may potentially promote tongue cancer metastasis.

KW - Areca nut

KW - Reactive oxygen species

KW - Tissue factor

KW - Tumor cells-induced platelet aggregation

KW - Tumor metastasis

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