Signaling of Macrophage Inflammatory Protein (MIP)-3β Facilitates Dengue Virus-Induced Microglial Cell Migration

Ming-Kai Jhan, Ting-Jing Shen, Po-Chun Tseng, Yung-Ting Wang, Chiou-Feng Lin

Research output: Contribution to journalArticle

Abstract

The infection by dengue virus (DENV) of microglia causes cell activation and migration via a mechanism involving viral entry, RNA release, and Toll-like receptor 3 signaling. In this study, we demonstrated that secreted chemotactic factors present in microglial conditioned medium (MCM) facilitated cell motility in the murine BV2 microglial cells. The pharmacological disruption of lipid rafts/caveolae reduced DENV-and ultraviolet (UV)-inactivated MCM-induced microglial cell migration. An antibody-based cytokine/chemokine array showed an increase in macrophage inflammatory protein (MIP)-3β in MCM produced using DENV-infected cells. The pharmacological inhibition of c-Jun N-terminal kinase (JNK) retarded UV-MCM-induced microglial cell migration. These results demonstrate that secreted MIP-3β and its effect on the JNK signaling pathways mediates DENV-induced BV2 microglial cell migration.

Original languageEnglish
JournalViruses
Volume10
Issue number12
DOIs
Publication statusPublished - Dec 1 2018

Fingerprint

Macrophage Inflammatory Proteins
Dengue Virus
Cell Movement
Conditioned Culture Medium
JNK Mitogen-Activated Protein Kinases
Toll-Like Receptor 3
Pharmacology
Caveolae
Chemotactic Factors
Viral RNA
Microglia
Chemokines
Cytokines
Lipids
Antibodies
Infection

Keywords

  • Caveolae
  • Dengue virus
  • MIP-3β
  • Microglia
  • Migration

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

Signaling of Macrophage Inflammatory Protein (MIP)-3β Facilitates Dengue Virus-Induced Microglial Cell Migration. / Jhan, Ming-Kai; Shen, Ting-Jing; Tseng, Po-Chun; Wang, Yung-Ting; Lin, Chiou-Feng.

In: Viruses, Vol. 10, No. 12, 01.12.2018.

Research output: Contribution to journalArticle

@article{619a4a0c5f2b4645becd21e096f98a19,
title = "Signaling of Macrophage Inflammatory Protein (MIP)-3β Facilitates Dengue Virus-Induced Microglial Cell Migration",
abstract = "The infection by dengue virus (DENV) of microglia causes cell activation and migration via a mechanism involving viral entry, RNA release, and Toll-like receptor 3 signaling. In this study, we demonstrated that secreted chemotactic factors present in microglial conditioned medium (MCM) facilitated cell motility in the murine BV2 microglial cells. The pharmacological disruption of lipid rafts/caveolae reduced DENV-and ultraviolet (UV)-inactivated MCM-induced microglial cell migration. An antibody-based cytokine/chemokine array showed an increase in macrophage inflammatory protein (MIP)-3β in MCM produced using DENV-infected cells. The pharmacological inhibition of c-Jun N-terminal kinase (JNK) retarded UV-MCM-induced microglial cell migration. These results demonstrate that secreted MIP-3β and its effect on the JNK signaling pathways mediates DENV-induced BV2 microglial cell migration.",
keywords = "Caveolae, Dengue virus, MIP-3β, Microglia, Migration",
author = "Ming-Kai Jhan and Ting-Jing Shen and Po-Chun Tseng and Yung-Ting Wang and Chiou-Feng Lin",
year = "2018",
month = "12",
day = "1",
doi = "10.3390/v10120690",
language = "English",
volume = "10",
journal = "Viruses",
issn = "1999-4915",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "12",

}

TY - JOUR

T1 - Signaling of Macrophage Inflammatory Protein (MIP)-3β Facilitates Dengue Virus-Induced Microglial Cell Migration

AU - Jhan, Ming-Kai

AU - Shen, Ting-Jing

AU - Tseng, Po-Chun

AU - Wang, Yung-Ting

AU - Lin, Chiou-Feng

PY - 2018/12/1

Y1 - 2018/12/1

N2 - The infection by dengue virus (DENV) of microglia causes cell activation and migration via a mechanism involving viral entry, RNA release, and Toll-like receptor 3 signaling. In this study, we demonstrated that secreted chemotactic factors present in microglial conditioned medium (MCM) facilitated cell motility in the murine BV2 microglial cells. The pharmacological disruption of lipid rafts/caveolae reduced DENV-and ultraviolet (UV)-inactivated MCM-induced microglial cell migration. An antibody-based cytokine/chemokine array showed an increase in macrophage inflammatory protein (MIP)-3β in MCM produced using DENV-infected cells. The pharmacological inhibition of c-Jun N-terminal kinase (JNK) retarded UV-MCM-induced microglial cell migration. These results demonstrate that secreted MIP-3β and its effect on the JNK signaling pathways mediates DENV-induced BV2 microglial cell migration.

AB - The infection by dengue virus (DENV) of microglia causes cell activation and migration via a mechanism involving viral entry, RNA release, and Toll-like receptor 3 signaling. In this study, we demonstrated that secreted chemotactic factors present in microglial conditioned medium (MCM) facilitated cell motility in the murine BV2 microglial cells. The pharmacological disruption of lipid rafts/caveolae reduced DENV-and ultraviolet (UV)-inactivated MCM-induced microglial cell migration. An antibody-based cytokine/chemokine array showed an increase in macrophage inflammatory protein (MIP)-3β in MCM produced using DENV-infected cells. The pharmacological inhibition of c-Jun N-terminal kinase (JNK) retarded UV-MCM-induced microglial cell migration. These results demonstrate that secreted MIP-3β and its effect on the JNK signaling pathways mediates DENV-induced BV2 microglial cell migration.

KW - Caveolae

KW - Dengue virus

KW - MIP-3β

KW - Microglia

KW - Migration

UR - http://www.scopus.com/inward/record.url?scp=85058314669&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85058314669&partnerID=8YFLogxK

U2 - 10.3390/v10120690

DO - 10.3390/v10120690

M3 - Article

VL - 10

JO - Viruses

JF - Viruses

SN - 1999-4915

IS - 12

ER -