Signal transducer and activator of transcription 1 in breast cancer: Analysis with tissue microarray

Shyr Ming Sheen-Chen, Chao Cheng Huang, Rei Ping Tang, Ching Hsiang Yang, Fong Fu Chou, Hock Liew Eng

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Background: Constitutively activated signal transducer and activator of transcription (STAT) proteins are found in various types of tumors. However, there is still very limited information about the role of STATs in breast cancer. The power of the tissue microarray analysis (TMA) technique is the capability of performing a series of analyses of thousands of specimens in a parallel fashion with minimal damage to the original blocks. This study was designed to use TMA in determing the STAT1 status in breast cancer tissues. Materials and Methods: Archival tissue specimens from 102 patients with primary invasive breast cancer were selected and STAT1 expression was analyzed using immunohistochemical staining with tissue microarray. The data of primary tumor staging, age, estrogen receptor status, lymph node status, histological grading and TNM staging were also collected. Results: There were 18 patients (17.6%) with 0 expression in STAT1, 29 patients (28.4%) with 1 expression in STAT1, 21 patients (20.6%) with 2 expression in STAT1 and 34 patients (33.4%) with 3 expression in STAT1. There was no significant relationship between STAT1 expression and age (p=0.203), estrogen receptor status (p=0.221), histological grading (p=0.861), primary tumor staging (p=0.918), lymph node status (p=0.53), or TNM staging (p=0.826). There was no survival difference noted among the four groups with different STAT1 expression (p=0.859). Conclusion: Immuno-histochemical staining with tissue microarray analysis was convenient and feasible for the analysis of STAT1 expression status in breast cancer. STAT1 expression did not show significant correlation with the overall survival rate.

Original languageEnglish
Pages (from-to)2481-2486
Number of pages6
JournalAnticancer Research
Volume27
Issue number4 B
Publication statusPublished - Jul 1 2007
Externally publishedYes

Fingerprint

Tissue Array Analysis
STAT1 Transcription Factor
Neoplasm Staging
Breast Neoplasms
Estrogen Receptors
Lymph Nodes
STAT Transcription Factors
Staining and Labeling
Survival Rate
Survival
Neoplasms

Keywords

  • Breast cancer
  • Signal transducer and activator of transcription
  • STAT1
  • Tissue microarray

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Sheen-Chen, S. M., Huang, C. C., Tang, R. P., Yang, C. H., Chou, F. F., & Eng, H. L. (2007). Signal transducer and activator of transcription 1 in breast cancer: Analysis with tissue microarray. Anticancer Research, 27(4 B), 2481-2486.

Signal transducer and activator of transcription 1 in breast cancer : Analysis with tissue microarray. / Sheen-Chen, Shyr Ming; Huang, Chao Cheng; Tang, Rei Ping; Yang, Ching Hsiang; Chou, Fong Fu; Eng, Hock Liew.

In: Anticancer Research, Vol. 27, No. 4 B, 01.07.2007, p. 2481-2486.

Research output: Contribution to journalArticle

Sheen-Chen, SM, Huang, CC, Tang, RP, Yang, CH, Chou, FF & Eng, HL 2007, 'Signal transducer and activator of transcription 1 in breast cancer: Analysis with tissue microarray', Anticancer Research, vol. 27, no. 4 B, pp. 2481-2486.
Sheen-Chen, Shyr Ming ; Huang, Chao Cheng ; Tang, Rei Ping ; Yang, Ching Hsiang ; Chou, Fong Fu ; Eng, Hock Liew. / Signal transducer and activator of transcription 1 in breast cancer : Analysis with tissue microarray. In: Anticancer Research. 2007 ; Vol. 27, No. 4 B. pp. 2481-2486.
@article{3aabfdaf5d1d4c4fbce7d2d77e8fbc16,
title = "Signal transducer and activator of transcription 1 in breast cancer: Analysis with tissue microarray",
abstract = "Background: Constitutively activated signal transducer and activator of transcription (STAT) proteins are found in various types of tumors. However, there is still very limited information about the role of STATs in breast cancer. The power of the tissue microarray analysis (TMA) technique is the capability of performing a series of analyses of thousands of specimens in a parallel fashion with minimal damage to the original blocks. This study was designed to use TMA in determing the STAT1 status in breast cancer tissues. Materials and Methods: Archival tissue specimens from 102 patients with primary invasive breast cancer were selected and STAT1 expression was analyzed using immunohistochemical staining with tissue microarray. The data of primary tumor staging, age, estrogen receptor status, lymph node status, histological grading and TNM staging were also collected. Results: There were 18 patients (17.6{\%}) with 0 expression in STAT1, 29 patients (28.4{\%}) with 1 expression in STAT1, 21 patients (20.6{\%}) with 2 expression in STAT1 and 34 patients (33.4{\%}) with 3 expression in STAT1. There was no significant relationship between STAT1 expression and age (p=0.203), estrogen receptor status (p=0.221), histological grading (p=0.861), primary tumor staging (p=0.918), lymph node status (p=0.53), or TNM staging (p=0.826). There was no survival difference noted among the four groups with different STAT1 expression (p=0.859). Conclusion: Immuno-histochemical staining with tissue microarray analysis was convenient and feasible for the analysis of STAT1 expression status in breast cancer. STAT1 expression did not show significant correlation with the overall survival rate.",
keywords = "Breast cancer, Signal transducer and activator of transcription, STAT1, Tissue microarray",
author = "Sheen-Chen, {Shyr Ming} and Huang, {Chao Cheng} and Tang, {Rei Ping} and Yang, {Ching Hsiang} and Chou, {Fong Fu} and Eng, {Hock Liew}",
year = "2007",
month = "7",
day = "1",
language = "English",
volume = "27",
pages = "2481--2486",
journal = "Anticancer Research",
issn = "0250-7005",
publisher = "International Institute of Anticancer Research",
number = "4 B",

}

TY - JOUR

T1 - Signal transducer and activator of transcription 1 in breast cancer

T2 - Analysis with tissue microarray

AU - Sheen-Chen, Shyr Ming

AU - Huang, Chao Cheng

AU - Tang, Rei Ping

AU - Yang, Ching Hsiang

AU - Chou, Fong Fu

AU - Eng, Hock Liew

PY - 2007/7/1

Y1 - 2007/7/1

N2 - Background: Constitutively activated signal transducer and activator of transcription (STAT) proteins are found in various types of tumors. However, there is still very limited information about the role of STATs in breast cancer. The power of the tissue microarray analysis (TMA) technique is the capability of performing a series of analyses of thousands of specimens in a parallel fashion with minimal damage to the original blocks. This study was designed to use TMA in determing the STAT1 status in breast cancer tissues. Materials and Methods: Archival tissue specimens from 102 patients with primary invasive breast cancer were selected and STAT1 expression was analyzed using immunohistochemical staining with tissue microarray. The data of primary tumor staging, age, estrogen receptor status, lymph node status, histological grading and TNM staging were also collected. Results: There were 18 patients (17.6%) with 0 expression in STAT1, 29 patients (28.4%) with 1 expression in STAT1, 21 patients (20.6%) with 2 expression in STAT1 and 34 patients (33.4%) with 3 expression in STAT1. There was no significant relationship between STAT1 expression and age (p=0.203), estrogen receptor status (p=0.221), histological grading (p=0.861), primary tumor staging (p=0.918), lymph node status (p=0.53), or TNM staging (p=0.826). There was no survival difference noted among the four groups with different STAT1 expression (p=0.859). Conclusion: Immuno-histochemical staining with tissue microarray analysis was convenient and feasible for the analysis of STAT1 expression status in breast cancer. STAT1 expression did not show significant correlation with the overall survival rate.

AB - Background: Constitutively activated signal transducer and activator of transcription (STAT) proteins are found in various types of tumors. However, there is still very limited information about the role of STATs in breast cancer. The power of the tissue microarray analysis (TMA) technique is the capability of performing a series of analyses of thousands of specimens in a parallel fashion with minimal damage to the original blocks. This study was designed to use TMA in determing the STAT1 status in breast cancer tissues. Materials and Methods: Archival tissue specimens from 102 patients with primary invasive breast cancer were selected and STAT1 expression was analyzed using immunohistochemical staining with tissue microarray. The data of primary tumor staging, age, estrogen receptor status, lymph node status, histological grading and TNM staging were also collected. Results: There were 18 patients (17.6%) with 0 expression in STAT1, 29 patients (28.4%) with 1 expression in STAT1, 21 patients (20.6%) with 2 expression in STAT1 and 34 patients (33.4%) with 3 expression in STAT1. There was no significant relationship between STAT1 expression and age (p=0.203), estrogen receptor status (p=0.221), histological grading (p=0.861), primary tumor staging (p=0.918), lymph node status (p=0.53), or TNM staging (p=0.826). There was no survival difference noted among the four groups with different STAT1 expression (p=0.859). Conclusion: Immuno-histochemical staining with tissue microarray analysis was convenient and feasible for the analysis of STAT1 expression status in breast cancer. STAT1 expression did not show significant correlation with the overall survival rate.

KW - Breast cancer

KW - Signal transducer and activator of transcription

KW - STAT1

KW - Tissue microarray

UR - http://www.scopus.com/inward/record.url?scp=34547742663&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34547742663&partnerID=8YFLogxK

M3 - Article

C2 - 17695542

AN - SCOPUS:34547742663

VL - 27

SP - 2481

EP - 2486

JO - Anticancer Research

JF - Anticancer Research

SN - 0250-7005

IS - 4 B

ER -