ShyA, a membrane protein for proper septation of hyphae in Streptomyces

TY - JOUR

T1 - ShyA, a membrane protein for proper septation of hyphae in Streptomyces

AU - Tseng, Shun-Fu

AU - Huang, Tzu-Wen

AU - Chen, Hsiuh-Wei

AU - Chern, Ming-Kai

AU - Tam, Ming-F.

AU - Teng, Shu-Chun

N1 - Export Date: 13 April 2016 CODEN: BBRCA 通訊地址: Teng, S.-C.; Department of Microbiology, College of Medicine, National Taiwan University, Taipei 10063, Taiwan; 電子郵件： scteng@ha.mc.ntu.edu.tw 化學物質/CAS: glutathione transferase, 50812-37-8; Membrane Proteins 參考文獻: Champness, W., (2000) Actinomycete Development, Antibiotic Production, and Phylogeny: Questions and Challenges, Prokaryotic Development, , American Society for Microbiology Washington, D.C; Chater, K.F., Taking a genetic scalpel to the Streptomyces colony (1998) Microbiology, 144, pp. 1465-1478; Kelemen, G.H., Buttner, M.J., Initiation of aerial mycelium formation in Streptomyces (1998) Curr. Opin. Microbiol., 1, pp. 656-662; Grantcharova, N., Lustig, U., Flardh, K., Dynamics of FtsZ assembly during sporulation in Streptomyces coelicolor A3(2) (2005) J. Bacteriol., 187, pp. 3227-3237; Schwedock, J., McCormick, J.R., Angert, E.R., Nodwell, J.R., Losick, R., Assembly of the cell division protein FtsZ into ladder-like structures in the aerial hyphae of Streptomyces coelicolor (1997) Mol. Microbiol., 25, pp. 847-858; McCormick, J.R., Su, E.P., Driks, A., Losick, R., Growth and viability of Streptomyces coelicolor mutant for the cell division gene ftsZ (1994) Mol. Microbiol., 14, pp. 243-254; Flardh, K., Leibovitz, E., Buttner, M.J., Chater, K.F., Generation of a non-sporulating strain of Streptomyces coelicolor A3(2) by the manipulation of a developmentally controlled ftsZ promoter (2000) Mol. Microbiol., 38, pp. 737-749; Grantcharova, N., Ubhayasekera, W., Mowbray, S.L., McCormick, J.R., Flardh, K., A missense mutation in ftsZ differentially affects vegetative and developmentally controlled cell division in Streptomyces coelicolor A3(2) (2003) Mol. Microbiol., 47, pp. 645-656; Chater, K.F., Regulation of sporulation in Streptomyces coelicolor A3(2): A checkpoint multiplex? (2001) Curr. Opin. Microbiol., 4, pp. 667-673; Wosten, H.A., Willey, J.M., Surface-active proteins enable microbial aerial hyphae to grow into the air (2000) Microbiology, 146, pp. 767-773; Keiser, T., Bibb, M.J., Buttner, M.J., Chater, K.F., Hopwood, D.A., (2000) Practical Streptomyces Genetics, , John Innes Foundation Norwich, United Kingdom; Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., Struhl, K., (1998) Current Protocols in Molecular Biology, , John Wiley New Jersey, USA; James, P., Halladay, J., Craig, E.A., Genomic libraries and a host strain designed for highly efficient two-hybrid selection in yeast (1996) Genetics, 144, pp. 1425-1436; Vieira, J., Messing, J., The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers (1982) Gene, 19, pp. 259-268; Bierman, M., Logan, R., O'Brien, K., Seno, E.T., Rao, R.N., Schoner, B.E., Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp (1992) Gene, 116, pp. 43-49; Yeh, H.I., Hsieh, C.H., Wang, L.Y., Tsai, S.P., Hsu, H.Y., Tam, M.F., Mass spectrometric analysis of rat liver cytosolic glutathione S-transferases: Modifications are limited to N-terminal processing (1995) Biochem. J., 308, pp. 69-75; Meijer, W.J., Serna-Rico, A., Salas, M., Characterization of the bacteriophage phi29-encoded protein p16.7: A membrane protein involved in phage DNA replication (2001) Mol. Microbiol., 39, pp. 731-746; Lin, Y.S., Kieser, H.M., Hopwood, D.A., Chen, C.W., The chromosomal DNA of Streptomyces lividans 66 is linear (1993) Mol. Microbiol., 10, pp. 923-933; Huang, C.H., Lin, Y.S., Yang, Y.L., Huang, S.W., Chen, C.W., The telomeres of Streptomyces chromosomes contain conserved palindromic sequences with potential to form complex secondary structures (1998) Mol. Microbiol., 28, pp. 905-916; Bao, K., Cohen, S.N., Terminal proteins essential for the replication of linear plasmids and chromosomes in Streptomyces (2001) Genes. Dev., 15, pp. 1518-1527; Yang, C.C., Huang, C.H., Li, C.Y., Tsay, Y.G., Lee, S.C., Chen, C.W., The terminal proteins of linear Streptomyces chromosomes and plasmids: A novel class of replication priming proteins (2002) Mol. Microbiol., 43, pp. 297-305; Bentley, S.D., Chater, K.F., Cerdeno-Tarraga, A.M., Challis, G.L., Thomson, N.R., James, K.D., Harris, D.E., Hopwood, D.A., Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2) (2002) Nature, 417, pp. 141-147; Hesketh, A.R., Chandra, G., Shaw, A.D., Rowland, J.J., Kell, D.B., Bibb, M.J., Chater, K.F., Primary and secondary metabolism, and post-translational protein modifications, as portrayed by proteomic analysis of Streptomyces coelicolor (2002) Mol. Microbiol., 46, pp. 917-932; Flardh, K., Growth polarity and cell division in Streptomyces (2003) Curr. Opin. Microbiol., 6, pp. 564-571

PY - 2006

Y1 - 2006

N2 - The life cycle of Streptomyces involves the formation of filamentous substrate and aerial hyphae. Following cessation of growth of an aerial hypha, multiple septation occurs at the tip to produce a chain of unigenomic spores. A gene, shyA, which influences several aspects of this growth, was isolated and partially characterized in Streptomyces coelicolor. The gene product is a representative of a well-conserved family of small actinomycete proteins. The shyA mutant sporulates normally but displays hyper septum formation and altered spore-chain morphology. Biochemical separation experiments and immunofluorescence staining demonstrated that the shyA gene product locates at cell membranes. Moreover, yeast two-hybrid screen and GST-pull-down assay showed that ShyA can interact with itself. Altogether, ShyA belongs to a new family of membrane-associated proteins which plays a role in morphological differentiation in actinomycetes. © 2006 Elsevier Inc. All rights reserved.

AB - The life cycle of Streptomyces involves the formation of filamentous substrate and aerial hyphae. Following cessation of growth of an aerial hypha, multiple septation occurs at the tip to produce a chain of unigenomic spores. A gene, shyA, which influences several aspects of this growth, was isolated and partially characterized in Streptomyces coelicolor. The gene product is a representative of a well-conserved family of small actinomycete proteins. The shyA mutant sporulates normally but displays hyper septum formation and altered spore-chain morphology. Biochemical separation experiments and immunofluorescence staining demonstrated that the shyA gene product locates at cell membranes. Moreover, yeast two-hybrid screen and GST-pull-down assay showed that ShyA can interact with itself. Altogether, ShyA belongs to a new family of membrane-associated proteins which plays a role in morphological differentiation in actinomycetes. © 2006 Elsevier Inc. All rights reserved.

KW - Actinomycetes

KW - Hyphae

KW - Membrane protein

KW - Spore

KW - Streptomyces

KW - gene product

KW - glutathione transferase

KW - membrane protein

KW - protein shyA

KW - unclassified drug

KW - animal cell

KW - article

KW - cell division

KW - cell membrane

KW - cell structure

KW - chemical analysis

KW - enzyme isolation

KW - immunofluorescence test

KW - nonhuman

KW - priority journal

KW - protein interaction

KW - separation technique

KW - Streptomyces coelicolor

KW - Amino Acid Sequence

KW - Cell Proliferation

KW - Cell Size

KW - Membrane Proteins

KW - Molecular Sequence Data

KW - Sequence Homology, Amino Acid

KW - Actinobacteria (class)

KW - Animalia

U2 - 10.1016/j.bbrc.2006.02.178

DO - 10.1016/j.bbrc.2006.02.178

M3 - Article

VL - 343

SP - 369

EP - 377

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -