ShyA, a membrane protein for proper septation of hyphae in Streptomyces

Shun-Fu Tseng, Tzu-Wen Huang, Hsiuh-Wei Chen, Ming-Kai Chern, Ming-F. Tam, Shu-Chun Teng

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The life cycle of Streptomyces involves the formation of filamentous substrate and aerial hyphae. Following cessation of growth of an aerial hypha, multiple septation occurs at the tip to produce a chain of unigenomic spores. A gene, shyA, which influences several aspects of this growth, was isolated and partially characterized in Streptomyces coelicolor. The gene product is a representative of a well-conserved family of small actinomycete proteins. The shyA mutant sporulates normally but displays hyper septum formation and altered spore-chain morphology. Biochemical separation experiments and immunofluorescence staining demonstrated that the shyA gene product locates at cell membranes. Moreover, yeast two-hybrid screen and GST-pull-down assay showed that ShyA can interact with itself. Altogether, ShyA belongs to a new family of membrane-associated proteins which plays a role in morphological differentiation in actinomycetes. © 2006 Elsevier Inc. All rights reserved.
Original languageEnglish
Pages (from-to)369-377
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume343
Issue number2
DOIs
Publication statusPublished - 2006
Externally publishedYes

Keywords

  • Actinomycetes
  • Hyphae
  • Membrane protein
  • Spore
  • Streptomyces
  • gene product
  • glutathione transferase
  • membrane protein
  • protein shyA
  • unclassified drug
  • animal cell
  • article
  • cell division
  • cell membrane
  • cell structure
  • chemical analysis
  • enzyme isolation
  • immunofluorescence test
  • nonhuman
  • priority journal
  • protein interaction
  • separation technique
  • Streptomyces coelicolor
  • Amino Acid Sequence
  • Cell Proliferation
  • Cell Size
  • Membrane Proteins
  • Molecular Sequence Data
  • Sequence Homology, Amino Acid
  • Actinobacteria (class)
  • Animalia

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