Short Communication: Porcine Sperm Motility Inhibitors Reduce the Acrosome Reaction in Boar Sperm

鄭 海倫(Hellen Jeng), 張 文章(Wen-Chang Chang)

Research output: Contribution to journalArticle

Abstract

By using a monoclonal antibody HS-63 vs. human acrosome and a secondary antibody conjugated with fluorescein isothiocyanate (FITC), it was demonstrated that two sperm motility inhibitors, SMI1 and SMI2, reduced the frequency of the spontaneous acrosome reaction; frequency of the spontaneous acrosome reaction in the control group was 11.7±1.8% (n=7), and in the SMI1-treated and the SMI2-treated groups 6.6±1.1% (P<0.05 vs. control, n=7) and 5.2±1.0% (P<0.01 vs. control, n=7), respectively.
Original languageUndefined/Unknown
Pages (from-to)265-268
Number of pages4
Journal中華民國獸醫學會雜誌
Volume23
Issue number3
Publication statusPublished - 1997

Keywords

  • 單株抗體HS-63
  • FITC螢光標記
  • Monoclonal antibody HS-63
  • FITC-immunolabel

Cite this

Short Communication: Porcine Sperm Motility Inhibitors Reduce the Acrosome Reaction in Boar Sperm. / 鄭海倫(Hellen Jeng); 張文章(Wen-Chang Chang).

In: 中華民國獸醫學會雜誌, Vol. 23, No. 3, 1997, p. 265-268.

Research output: Contribution to journalArticle

鄭海倫(Hellen Jeng) ; 張文章(Wen-Chang Chang). / Short Communication: Porcine Sperm Motility Inhibitors Reduce the Acrosome Reaction in Boar Sperm. In: 中華民國獸醫學會雜誌. 1997 ; Vol. 23, No. 3. pp. 265-268.
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title = "Short Communication: Porcine Sperm Motility Inhibitors Reduce the Acrosome Reaction in Boar Sperm",
abstract = "本實驗利用只會結合在完整精子頭帽上的單株抗體 HS-63,探討由豬精漿中分離出之精子活動力抑制蛋白 (SM I1與 SMI2) 對精子頭帽反應的作用,並選用接上FITC 螢光劑的第二抗體作為標記,以使在螢光顯微鏡下觀察,結果發現 SMI1與SMI2 可降低白發性頭帽反應的發生率,其中控制組自發性頭帽反應的發生率為 11.7 士1.8{\%} ,而 SMI1與 SMI2 的發生率分別是 6.6士 l.l{\%} (P < 0.05, n = 7) 與 5.2 士1.0{\%} (p < 0.01, n = 7)",
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author = "鄭, {海倫(Hellen Jeng)} and 張, {文章(Wen-Chang Chang)}",
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pages = "265--268",
journal = "中華民國獸醫學會雜誌",
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T1 - Short Communication: Porcine Sperm Motility Inhibitors Reduce the Acrosome Reaction in Boar Sperm

AU - 鄭, 海倫(Hellen Jeng)

AU - 張, 文章(Wen-Chang Chang)

PY - 1997

Y1 - 1997

N2 - 本實驗利用只會結合在完整精子頭帽上的單株抗體 HS-63,探討由豬精漿中分離出之精子活動力抑制蛋白 (SM I1與 SMI2) 對精子頭帽反應的作用,並選用接上FITC 螢光劑的第二抗體作為標記,以使在螢光顯微鏡下觀察,結果發現 SMI1與SMI2 可降低白發性頭帽反應的發生率,其中控制組自發性頭帽反應的發生率為 11.7 士1.8% ,而 SMI1與 SMI2 的發生率分別是 6.6士 l.l% (P < 0.05, n = 7) 與 5.2 士1.0% (p < 0.01, n = 7)

AB - 本實驗利用只會結合在完整精子頭帽上的單株抗體 HS-63,探討由豬精漿中分離出之精子活動力抑制蛋白 (SM I1與 SMI2) 對精子頭帽反應的作用,並選用接上FITC 螢光劑的第二抗體作為標記,以使在螢光顯微鏡下觀察,結果發現 SMI1與SMI2 可降低白發性頭帽反應的發生率,其中控制組自發性頭帽反應的發生率為 11.7 士1.8% ,而 SMI1與 SMI2 的發生率分別是 6.6士 l.l% (P < 0.05, n = 7) 與 5.2 士1.0% (p < 0.01, n = 7)

KW - 單株抗體HS-63

KW - FITC螢光標記

KW - Monoclonal antibody HS-63

KW - FITC-immunolabel

M3 - 文章

VL - 23

SP - 265

EP - 268

JO - 中華民國獸醫學會雜誌

JF - 中華民國獸醫學會雜誌

SN - 0253-9179

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ER -