Abstract
Purpose: To report a successful pregnancy from cryopreserved sibling oocytes and intracytoplasmic sperm injection (ICSI) for an infertile couple with an unexpectedly low fertilization rate in the fresh in vitro fertilization (IVF) cycle. Methods: The woman had bilateral tubal obstruction and polycystic ovarian syndrome. The man had normal semen parameters. The couple underwent a cycle of controlled ovarian hyperstimulation in that 20 oocytes were retrieved. Twelve oocytes were conventionally inseminated and eight were cryopreserved using a slow freezing method. However, only one oocyte was fertilized, and no pregnancy was achieved. In the next cycle, the frozen oocytes were thawed and ICSI was performed. Results: After thawing, seven oocytes (88%) survived and one was damaged. Six were at the metaphase II stage and were injected. Five (83%) achieved normal fertilization, and all of them cleaved (100%). After replacement of the embryos, a singleton pregnancy developed. A healthy female baby was delivered at term. Karyotyping revealed 46, XX. Conclusions: In addition to well-known indications, cryopreservation of excess sibling oocytes for patients receiving IVF has a possible advantage of preventing unexpectedly low fertilization rate or fertilization failures.
| Original language | English |
|---|---|
| Pages (from-to) | 367-369 |
| Number of pages | 3 |
| Journal | Journal of Assisted Reproduction and Genetics |
| Volume | 21 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - Oct 1 2004 |
| Externally published | Yes |
Fingerprint
Keywords
- Cryopreserved oocytes
- intracytoplasmic sperm injection
- unexpected fertilization failures
ASJC Scopus subject areas
- Obstetrics and Gynaecology
- Developmental Biology
- Genetics
- Reproductive Medicine
Cite this
Short communication : Cryopreserved sibling oocytes and intracytoplasmic sperm injection rescue unexpectedly poor fertilization in conventional in vitro fertilization. / Chen, Shee Uan; Lien, Yih Ron; Chang, Li Jung; Tsai, Yi Yi; Ho, Hong Nerng; Yang, Yu Shih.
In: Journal of Assisted Reproduction and Genetics, Vol. 21, No. 10, 01.10.2004, p. 367-369.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Short communication
T2 - Cryopreserved sibling oocytes and intracytoplasmic sperm injection rescue unexpectedly poor fertilization in conventional in vitro fertilization
AU - Chen, Shee Uan
AU - Lien, Yih Ron
AU - Chang, Li Jung
AU - Tsai, Yi Yi
AU - Ho, Hong Nerng
AU - Yang, Yu Shih
PY - 2004/10/1
Y1 - 2004/10/1
N2 - Purpose: To report a successful pregnancy from cryopreserved sibling oocytes and intracytoplasmic sperm injection (ICSI) for an infertile couple with an unexpectedly low fertilization rate in the fresh in vitro fertilization (IVF) cycle. Methods: The woman had bilateral tubal obstruction and polycystic ovarian syndrome. The man had normal semen parameters. The couple underwent a cycle of controlled ovarian hyperstimulation in that 20 oocytes were retrieved. Twelve oocytes were conventionally inseminated and eight were cryopreserved using a slow freezing method. However, only one oocyte was fertilized, and no pregnancy was achieved. In the next cycle, the frozen oocytes were thawed and ICSI was performed. Results: After thawing, seven oocytes (88%) survived and one was damaged. Six were at the metaphase II stage and were injected. Five (83%) achieved normal fertilization, and all of them cleaved (100%). After replacement of the embryos, a singleton pregnancy developed. A healthy female baby was delivered at term. Karyotyping revealed 46, XX. Conclusions: In addition to well-known indications, cryopreservation of excess sibling oocytes for patients receiving IVF has a possible advantage of preventing unexpectedly low fertilization rate or fertilization failures.
AB - Purpose: To report a successful pregnancy from cryopreserved sibling oocytes and intracytoplasmic sperm injection (ICSI) for an infertile couple with an unexpectedly low fertilization rate in the fresh in vitro fertilization (IVF) cycle. Methods: The woman had bilateral tubal obstruction and polycystic ovarian syndrome. The man had normal semen parameters. The couple underwent a cycle of controlled ovarian hyperstimulation in that 20 oocytes were retrieved. Twelve oocytes were conventionally inseminated and eight were cryopreserved using a slow freezing method. However, only one oocyte was fertilized, and no pregnancy was achieved. In the next cycle, the frozen oocytes were thawed and ICSI was performed. Results: After thawing, seven oocytes (88%) survived and one was damaged. Six were at the metaphase II stage and were injected. Five (83%) achieved normal fertilization, and all of them cleaved (100%). After replacement of the embryos, a singleton pregnancy developed. A healthy female baby was delivered at term. Karyotyping revealed 46, XX. Conclusions: In addition to well-known indications, cryopreservation of excess sibling oocytes for patients receiving IVF has a possible advantage of preventing unexpectedly low fertilization rate or fertilization failures.
KW - Cryopreserved oocytes
KW - intracytoplasmic sperm injection
KW - unexpected fertilization failures
UR - http://www.scopus.com/inward/record.url?scp=16644403640&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=16644403640&partnerID=8YFLogxK
U2 - 10.1023/B:JARG.0000046205.15721.d4
DO - 10.1023/B:JARG.0000046205.15721.d4
M3 - Article
C2 - 15587141
AN - SCOPUS:16644403640
VL - 21
SP - 367
EP - 369
JO - Journal of Assisted Reproduction and Genetics
JF - Journal of Assisted Reproduction and Genetics
SN - 1058-0468
IS - 10
ER -