Sex differences in the electrophysiological characteristics of pulmonary veins and left atrium and their clinical implication in atrial fibrillation

Wen Chin Tsai, Yao Chang Chen, Yung-Kuo Lin, Shih A. Chen, Yi-Jen Chen

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Background-Sex and the autonomic nervous system play critical roles in the pathophysiology of atrial fibrillation (AF). Sex differences in electrophysiological characteristics of the pulmonary veins (PVs, AF initiator) and left atrium (LA, AF substrate) are not clear. Methods and Results-Conventional microelectrodes were used to record the action potential in isolated PV and LA tissue preparations from male and female (age, 8≈10 months) rabbits before and after drug administration (adenosine, acetylcholine, and isoproterenol). Male PVs (n=7) had a higher spontaneous beating rate (1.7±0.2 versus 1.2±0.1 Hz, P=0.021) and incidence of burst firing (72% versus 11%, P=0.038) than female PVs (n=9). Male PVs without spontaneous activity (n=10) and the LA (n=11) had longer action potential durations than female PVs (n=9) and LA (n=9). Additionally, male PVs had a more-positive resting membrane potential (79±3 versus 84±2 mV, P=0.022). Isoproterenol (3 μmol/L) increased the delayed afterdepolarizations to a greater extent in male than in female PVs. In PVs without spontaneous activity or LA, isoproterenol (0.1 and 3 μmol/L) consistently shortened the action potential durations in females but not in males. Acetylcholine (5.5 μmol/L) decreased the spontaneous activity of PVs and shortened the action potential durations in both groups. Adenosine (10 μmol/L) also similarly decreased the spontaneous activity of PVs and delayed afterdepolarizations in both groups. Conclusions-There are significant sex differences in PV and LA action potential characteristics in rabbits. The higher amplitude of delayed afterdepolarizations after isoproterenol superfusion in male PVs may contribute to sex-related arrhythmogenesis.

Original languageEnglish
Pages (from-to)550-559
Number of pages10
JournalCirculation: Arrhythmia and Electrophysiology
Volume4
Issue number4
DOIs
Publication statusPublished - Aug 2011

Fingerprint

Pulmonary Veins
Heart Atria
Sex Characteristics
Atrial Fibrillation
Action Potentials
Isoproterenol
Adenosine
Acetylcholine
Rabbits
Autonomic Nervous System
Microelectrodes
Membrane Potentials
Incidence
Pharmaceutical Preparations

Keywords

  • Atrial fibrillation
  • Left atrium
  • Pulmonary veins
  • Sex

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)
  • Medicine(all)

Cite this

@article{c5ab291445874bb6972c5784b794f841,
title = "Sex differences in the electrophysiological characteristics of pulmonary veins and left atrium and their clinical implication in atrial fibrillation",
abstract = "Background-Sex and the autonomic nervous system play critical roles in the pathophysiology of atrial fibrillation (AF). Sex differences in electrophysiological characteristics of the pulmonary veins (PVs, AF initiator) and left atrium (LA, AF substrate) are not clear. Methods and Results-Conventional microelectrodes were used to record the action potential in isolated PV and LA tissue preparations from male and female (age, 8≈10 months) rabbits before and after drug administration (adenosine, acetylcholine, and isoproterenol). Male PVs (n=7) had a higher spontaneous beating rate (1.7±0.2 versus 1.2±0.1 Hz, P=0.021) and incidence of burst firing (72{\%} versus 11{\%}, P=0.038) than female PVs (n=9). Male PVs without spontaneous activity (n=10) and the LA (n=11) had longer action potential durations than female PVs (n=9) and LA (n=9). Additionally, male PVs had a more-positive resting membrane potential (79±3 versus 84±2 mV, P=0.022). Isoproterenol (3 μmol/L) increased the delayed afterdepolarizations to a greater extent in male than in female PVs. In PVs without spontaneous activity or LA, isoproterenol (0.1 and 3 μmol/L) consistently shortened the action potential durations in females but not in males. Acetylcholine (5.5 μmol/L) decreased the spontaneous activity of PVs and shortened the action potential durations in both groups. Adenosine (10 μmol/L) also similarly decreased the spontaneous activity of PVs and delayed afterdepolarizations in both groups. Conclusions-There are significant sex differences in PV and LA action potential characteristics in rabbits. The higher amplitude of delayed afterdepolarizations after isoproterenol superfusion in male PVs may contribute to sex-related arrhythmogenesis.",
keywords = "Atrial fibrillation, Left atrium, Pulmonary veins, Sex",
author = "Tsai, {Wen Chin} and Chen, {Yao Chang} and Yung-Kuo Lin and Chen, {Shih A.} and Yi-Jen Chen",
year = "2011",
month = "8",
doi = "10.1161/CIRCEP.111.961995",
language = "English",
volume = "4",
pages = "550--559",
journal = "Circulation: Arrhythmia and Electrophysiology",
issn = "1941-3149",
publisher = "Lippincott Williams and Wilkins",
number = "4",

}

TY - JOUR

T1 - Sex differences in the electrophysiological characteristics of pulmonary veins and left atrium and their clinical implication in atrial fibrillation

AU - Tsai, Wen Chin

AU - Chen, Yao Chang

AU - Lin, Yung-Kuo

AU - Chen, Shih A.

AU - Chen, Yi-Jen

PY - 2011/8

Y1 - 2011/8

N2 - Background-Sex and the autonomic nervous system play critical roles in the pathophysiology of atrial fibrillation (AF). Sex differences in electrophysiological characteristics of the pulmonary veins (PVs, AF initiator) and left atrium (LA, AF substrate) are not clear. Methods and Results-Conventional microelectrodes were used to record the action potential in isolated PV and LA tissue preparations from male and female (age, 8≈10 months) rabbits before and after drug administration (adenosine, acetylcholine, and isoproterenol). Male PVs (n=7) had a higher spontaneous beating rate (1.7±0.2 versus 1.2±0.1 Hz, P=0.021) and incidence of burst firing (72% versus 11%, P=0.038) than female PVs (n=9). Male PVs without spontaneous activity (n=10) and the LA (n=11) had longer action potential durations than female PVs (n=9) and LA (n=9). Additionally, male PVs had a more-positive resting membrane potential (79±3 versus 84±2 mV, P=0.022). Isoproterenol (3 μmol/L) increased the delayed afterdepolarizations to a greater extent in male than in female PVs. In PVs without spontaneous activity or LA, isoproterenol (0.1 and 3 μmol/L) consistently shortened the action potential durations in females but not in males. Acetylcholine (5.5 μmol/L) decreased the spontaneous activity of PVs and shortened the action potential durations in both groups. Adenosine (10 μmol/L) also similarly decreased the spontaneous activity of PVs and delayed afterdepolarizations in both groups. Conclusions-There are significant sex differences in PV and LA action potential characteristics in rabbits. The higher amplitude of delayed afterdepolarizations after isoproterenol superfusion in male PVs may contribute to sex-related arrhythmogenesis.

AB - Background-Sex and the autonomic nervous system play critical roles in the pathophysiology of atrial fibrillation (AF). Sex differences in electrophysiological characteristics of the pulmonary veins (PVs, AF initiator) and left atrium (LA, AF substrate) are not clear. Methods and Results-Conventional microelectrodes were used to record the action potential in isolated PV and LA tissue preparations from male and female (age, 8≈10 months) rabbits before and after drug administration (adenosine, acetylcholine, and isoproterenol). Male PVs (n=7) had a higher spontaneous beating rate (1.7±0.2 versus 1.2±0.1 Hz, P=0.021) and incidence of burst firing (72% versus 11%, P=0.038) than female PVs (n=9). Male PVs without spontaneous activity (n=10) and the LA (n=11) had longer action potential durations than female PVs (n=9) and LA (n=9). Additionally, male PVs had a more-positive resting membrane potential (79±3 versus 84±2 mV, P=0.022). Isoproterenol (3 μmol/L) increased the delayed afterdepolarizations to a greater extent in male than in female PVs. In PVs without spontaneous activity or LA, isoproterenol (0.1 and 3 μmol/L) consistently shortened the action potential durations in females but not in males. Acetylcholine (5.5 μmol/L) decreased the spontaneous activity of PVs and shortened the action potential durations in both groups. Adenosine (10 μmol/L) also similarly decreased the spontaneous activity of PVs and delayed afterdepolarizations in both groups. Conclusions-There are significant sex differences in PV and LA action potential characteristics in rabbits. The higher amplitude of delayed afterdepolarizations after isoproterenol superfusion in male PVs may contribute to sex-related arrhythmogenesis.

KW - Atrial fibrillation

KW - Left atrium

KW - Pulmonary veins

KW - Sex

UR - http://www.scopus.com/inward/record.url?scp=80054021936&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80054021936&partnerID=8YFLogxK

U2 - 10.1161/CIRCEP.111.961995

DO - 10.1161/CIRCEP.111.961995

M3 - Article

C2 - 21659634

AN - SCOPUS:80054021936

VL - 4

SP - 550

EP - 559

JO - Circulation: Arrhythmia and Electrophysiology

JF - Circulation: Arrhythmia and Electrophysiology

SN - 1941-3149

IS - 4

ER -