Sesamol reduces the atherogenicity of electronegative L5 LDL in vivo and in vitro

Wei Yu Chen, Fang Yu Chen, An Sheng Lee, Kuan Hsiang Ting, Chia Ming Chang, Jing Fang Hsu, Wei Shine Lee, Joen Rong Sheu, Chu Huang Chen, Ming Yi Shen

Research output: Contribution to journalArticle

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Abstract

Highly electronegative low-density lipoprotein (LDL) L5 induces endothelial cell (EC) apoptosis, which leads to the development of atherosclerosis. We examined the effects of sesamol (1), a natural organic component of sesame oil, on plasma L5 levels and atherosclerosis development in a rodent model and on the L5-induced apoptosis of ECs. Syrian hamsters, which have an LDL profile similar to that of humans, were fed a normal chow diet (control), a high-fat diet (HFD), or a HFD supplemented with the administration of 50 or 100 mg/kg of 1 via oral gavage (HFD+1) for 16 weeks (n = 8 per group). Hamsters in the HFD+1 groups had reduced plasma L5 levels when compared with the HFD group. Oil Red O staining showed that atherosclerotic lesion size was markedly reduced in the aortic arch of hamsters in the HFD+1 groups when compared with that in the HFD group. In human aortic ECs, 0.3-3 μM 1 blocked L5-induced apoptosis in a dose-dependent manner. Further mechanistic studies showed that 1 inhibited the L5-induced lectin-like oxidized LDL receptor-1 (LOX-1)-dependent phosphorylation of p38 MAPK and activation of caspase-3 and increased phosphorylation of eNOS and Akt. Our findings suggest that sesamol (1) protects against atherosclerosis by reducing L5-induced atherogenicity.

Original languageEnglish
Pages (from-to)225-233
Number of pages9
JournalJournal of Natural Products
Volume78
Issue number2
DOIs
Publication statusPublished - Feb 27 2015

Fingerprint

High Fat Diet
Nutrition
LDL Lipoproteins
Fats
Atherosclerosis
Phosphorylation
Apoptosis
Cricetinae
Class E Scavenger Receptors
Sesame Oil
Plasmas
Mesocricetus
p38 Mitogen-Activated Protein Kinases
sesamol
oxidized low density lipoprotein
In Vitro Techniques
Thoracic Aorta
Endothelial cells
Caspase 3
Arches

ASJC Scopus subject areas

  • Drug Discovery
  • Pharmacology
  • Pharmaceutical Science
  • Analytical Chemistry
  • Organic Chemistry
  • Molecular Medicine
  • Complementary and alternative medicine
  • Medicine(all)

Cite this

Chen, W. Y., Chen, F. Y., Lee, A. S., Ting, K. H., Chang, C. M., Hsu, J. F., ... Shen, M. Y. (2015). Sesamol reduces the atherogenicity of electronegative L5 LDL in vivo and in vitro. Journal of Natural Products, 78(2), 225-233. https://doi.org/10.1021/np500700z

Sesamol reduces the atherogenicity of electronegative L5 LDL in vivo and in vitro. / Chen, Wei Yu; Chen, Fang Yu; Lee, An Sheng; Ting, Kuan Hsiang; Chang, Chia Ming; Hsu, Jing Fang; Lee, Wei Shine; Sheu, Joen Rong; Chen, Chu Huang; Shen, Ming Yi.

In: Journal of Natural Products, Vol. 78, No. 2, 27.02.2015, p. 225-233.

Research output: Contribution to journalArticle

Chen, WY, Chen, FY, Lee, AS, Ting, KH, Chang, CM, Hsu, JF, Lee, WS, Sheu, JR, Chen, CH & Shen, MY 2015, 'Sesamol reduces the atherogenicity of electronegative L5 LDL in vivo and in vitro', Journal of Natural Products, vol. 78, no. 2, pp. 225-233. https://doi.org/10.1021/np500700z
Chen, Wei Yu ; Chen, Fang Yu ; Lee, An Sheng ; Ting, Kuan Hsiang ; Chang, Chia Ming ; Hsu, Jing Fang ; Lee, Wei Shine ; Sheu, Joen Rong ; Chen, Chu Huang ; Shen, Ming Yi. / Sesamol reduces the atherogenicity of electronegative L5 LDL in vivo and in vitro. In: Journal of Natural Products. 2015 ; Vol. 78, No. 2. pp. 225-233.
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