Sesamin attenuates intercellular cell adhesion molecule-1 expression in vitro in TNF-α-treated human aortic endothelial cells and in vivo in apolipoprotein-E-deficient mice

Wen-Huey Wu, Shu-Huei Wang, I.-I. Kuan, Ya-S. Kao, Pei-Jhen Wu, Chan-Jung Liang, Hsiung-Fei Chien, Chiu H. Kao, Ching-Jang Huang, Yuh-Lien Chen

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Sesame lignans have antioxidative and anti-inflammatory properties. We focused on the effects of the lignans sesamin and sesamol on the expression of endothelial-leukocyte adhesion molecules in tumor necrosis factor-α (TNF-α)-treated human aortic endothelial cells (HAECs). When HAECs were pretreated with sesamin (10 or 100 μM), the TNF-α-induced expression of intercellular cell adhesion molecule-1 (ICAM-1) was significantly reduced (35 or 70% decrease, respectively) by Western blotting. Sesamol was less effective at inhibiting ICAM-1 expression (30% decrease at 100 μM). Sesamin and sesamol reduced the marked TNF-α-induced increase in human antigen R (HuR) translocation and the interaction between HuR and the 3'UTR of ICAM-1 mRNA. Both significantly reduced the binding of monocytes to TNF-α-stimulated HAECs. Sesamin significantly attenuated TNF-α-induced ICAM-1 expression and cell adhesion by downregulation of extracellular signal-regulated kinase 1/2 and p38. Furthermore, in vivo, sesamin attenuated intimal thickening and ICAM-1 expression seen in aortas of apolipoprotein-E-deficient mice. Taken together, these data suggest that sesamin inhibits TNF-α -induced extracellular signal-regulated kinase/p38 phosphorylation, nuclear translocation of NF-κB p65, cytoplasmic translocalization of HuR and thereby suppresses ICAM-1 expression, resulting in reduced adhesion of leukocytes. These results also suggest that sesamin may prevent the development of atherosclerosis and inflammatory responses. © 2010 WILEY-VCH Verlag GmbH & Co.
Original languageEnglish
Pages (from-to)1340-1350
Number of pages11
JournalMolecular Nutrition and Food Research
Volume54
Issue number9
DOIs
Publication statusPublished - 2010
Externally publishedYes

Fingerprint

apolipoprotein E
tumor necrosis factors
Cell Adhesion Molecules
Apolipoproteins E
Intercellular Adhesion Molecule-1
cell adhesion
endothelial cells
Endothelial Cells
mice
Tumor Necrosis Factor-alpha
Lignans
lignans
antigens
mitogen-activated protein kinase
Antigens
adhesion
leukocytes
Tunica Intima
Sesamum
Mitogen-Activated Protein Kinase 3

Keywords

  • Atherosclerosis
  • Endothelial cell
  • Intercellular cell adhesion molecule-1
  • NF-κb
  • Sesamin
  • 1,3 benzodioxole derivative
  • 1,3 dioxolane derivative
  • antioxidant
  • apolipoprotein E
  • ELAV-like protein 1
  • HuR protein
  • intercellular adhesion molecule 1
  • lignan
  • membrane antigen
  • messenger RNA
  • nonsteroid antiinflammatory agent
  • phenol derivative
  • RNA binding protein
  • sesamin
  • sesamol
  • small interfering RNA
  • tumor necrosis factor alpha
  • animal
  • aorta
  • article
  • atherosclerosis
  • cell culture
  • cell line
  • comparative study
  • cytology
  • drug effect
  • gene expression regulation
  • genetics
  • human
  • metabolism
  • monocyte
  • mouse
  • mouse mutant
  • pathology
  • randomization
  • signal transduction
  • vascular endothelium
  • Animals
  • Anti-Inflammatory Agents, Non-Steroidal
  • Antigens, Surface
  • Antioxidants
  • Aorta
  • Apolipoproteins E
  • Benzodioxoles
  • Cell Line
  • Cells, Cultured
  • Dioxoles
  • Endothelium, Vascular
  • Gene Expression Regulation
  • Humans
  • Intercellular Adhesion Molecule-1
  • Lignans
  • Mice
  • Mice, Knockout
  • Monocytes
  • Phenols
  • Random Allocation
  • RNA, Messenger
  • RNA, Small Interfering
  • RNA-Binding Proteins
  • Signal Transduction
  • Tumor Necrosis Factor-alpha
  • Mus
  • Sesamum indicum

Cite this

Sesamin attenuates intercellular cell adhesion molecule-1 expression in vitro in TNF-α-treated human aortic endothelial cells and in vivo in apolipoprotein-E-deficient mice. / Wu, Wen-Huey; Wang, Shu-Huei; Kuan, I.-I.; Kao, Ya-S.; Wu, Pei-Jhen; Liang, Chan-Jung; Chien, Hsiung-Fei; Kao, Chiu H.; Huang, Ching-Jang; Chen, Yuh-Lien.

In: Molecular Nutrition and Food Research, Vol. 54, No. 9, 2010, p. 1340-1350.

Research output: Contribution to journalArticle

Wu, Wen-Huey ; Wang, Shu-Huei ; Kuan, I.-I. ; Kao, Ya-S. ; Wu, Pei-Jhen ; Liang, Chan-Jung ; Chien, Hsiung-Fei ; Kao, Chiu H. ; Huang, Ching-Jang ; Chen, Yuh-Lien. / Sesamin attenuates intercellular cell adhesion molecule-1 expression in vitro in TNF-α-treated human aortic endothelial cells and in vivo in apolipoprotein-E-deficient mice. In: Molecular Nutrition and Food Research. 2010 ; Vol. 54, No. 9. pp. 1340-1350.
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title = "Sesamin attenuates intercellular cell adhesion molecule-1 expression in vitro in TNF-α-treated human aortic endothelial cells and in vivo in apolipoprotein-E-deficient mice",
abstract = "Sesame lignans have antioxidative and anti-inflammatory properties. We focused on the effects of the lignans sesamin and sesamol on the expression of endothelial-leukocyte adhesion molecules in tumor necrosis factor-α (TNF-α)-treated human aortic endothelial cells (HAECs). When HAECs were pretreated with sesamin (10 or 100 μM), the TNF-α-induced expression of intercellular cell adhesion molecule-1 (ICAM-1) was significantly reduced (35 or 70{\%} decrease, respectively) by Western blotting. Sesamol was less effective at inhibiting ICAM-1 expression (30{\%} decrease at 100 μM). Sesamin and sesamol reduced the marked TNF-α-induced increase in human antigen R (HuR) translocation and the interaction between HuR and the 3'UTR of ICAM-1 mRNA. Both significantly reduced the binding of monocytes to TNF-α-stimulated HAECs. Sesamin significantly attenuated TNF-α-induced ICAM-1 expression and cell adhesion by downregulation of extracellular signal-regulated kinase 1/2 and p38. Furthermore, in vivo, sesamin attenuated intimal thickening and ICAM-1 expression seen in aortas of apolipoprotein-E-deficient mice. Taken together, these data suggest that sesamin inhibits TNF-α -induced extracellular signal-regulated kinase/p38 phosphorylation, nuclear translocation of NF-κB p65, cytoplasmic translocalization of HuR and thereby suppresses ICAM-1 expression, resulting in reduced adhesion of leukocytes. These results also suggest that sesamin may prevent the development of atherosclerosis and inflammatory responses. {\circledC} 2010 WILEY-VCH Verlag GmbH & Co.",
keywords = "Atherosclerosis, Endothelial cell, Intercellular cell adhesion molecule-1, NF-κb, Sesamin, 1,3 benzodioxole derivative, 1,3 dioxolane derivative, antioxidant, apolipoprotein E, ELAV-like protein 1, HuR protein, intercellular adhesion molecule 1, lignan, membrane antigen, messenger RNA, nonsteroid antiinflammatory agent, phenol derivative, RNA binding protein, sesamin, sesamol, small interfering RNA, tumor necrosis factor alpha, animal, aorta, article, atherosclerosis, cell culture, cell line, comparative study, cytology, drug effect, gene expression regulation, genetics, human, metabolism, monocyte, mouse, mouse mutant, pathology, randomization, signal transduction, vascular endothelium, Animals, Anti-Inflammatory Agents, Non-Steroidal, Antigens, Surface, Antioxidants, Aorta, Apolipoproteins E, Benzodioxoles, Cell Line, Cells, Cultured, Dioxoles, Endothelium, Vascular, Gene Expression Regulation, Humans, Intercellular Adhesion Molecule-1, Lignans, Mice, Mice, Knockout, Monocytes, Phenols, Random Allocation, RNA, Messenger, RNA, Small Interfering, RNA-Binding Proteins, Signal Transduction, Tumor Necrosis Factor-alpha, Mus, Sesamum indicum",
author = "Wen-Huey Wu and Shu-Huei Wang and I.-I. Kuan and Ya-S. Kao and Pei-Jhen Wu and Chan-Jung Liang and Hsiung-Fei Chien and Kao, {Chiu H.} and Ching-Jang Huang and Yuh-Lien Chen",
note = "被引用次數:10 Export Date: 16 March 2016 通訊地址: Chen, Y.-L.; Department of Anatomy and Cell Biology, College of Medicine, National Taiwan University, No. 1, Section 1, Ren-Ai Rd., Taipei 100, Taiwan; 電子郵件: ylchenv@ntu.edu.tw 化學物質/CAS: intercellular adhesion molecule 1, 126547-89-5; sesamin, 607-80-7, 7076-24-6; sesamol, 533-31-3; Anti-Inflammatory Agents, Non-Steroidal; Antigens, Surface; Antioxidants; Apolipoproteins E; Benzodioxoles; Dioxoles; ELAV-like protein 1; Intercellular Adhesion Molecule-1, 126547-89-5; Lignans; Phenols; RNA, Messenger; RNA, Small Interfering; RNA-Binding Proteins; Tumor Necrosis Factor-alpha; sesamin, 607-80-7; sesamol, 533-31-3 參考文獻: Budowski, P., Markley, K.S., The chemical and physiological properties of sesame oil (1951) Chem. Rev, 48, pp. 125-151; Akimoto, K., Kitagawa, Y., Akamatsu, T., Hirose, N., Protective effects of sesamin against liver damage caused by alcohol or carbon tetrachloride in rodents (1993) Ann. Nutr. Metab, 37, pp. 218-224; Hirose, N., Doi, F., Ueki, T., Akazawa, K., Suppressive effect of sesamin against 7,12-dimethylbenz-anthracene induced rat mammary carcinogenesis (1992) Anticancer Res, 12, pp. 1259-1265; Chavali, S.R., Zhong, W.W., Forse, R.A., Dietary alphalinolenic acid increases TNF-alpha, and decreases IL-6, IL-10 in response to LPS: Effects of sesamin on the delta-5 desaturation of o6 and o3 fatty acids in mice (1998) Essent. Fatty Acids, 58, pp. 185-191. , Prostaglandins Leuko; Chavali, S.R., Utsunomiya, T., Forse, R.A., Increased survival after cecal ligation and puncture in mice consuming diets enriched with sesame seed oil (2001) Crit. Care Med, 29, pp. 140-143; Huo, R.C., Chen, H.L., Tzen, J.T., Jeng, K.C., Effect of sesame antioxidants on LPS-induced NO production by BV2 microglial cells (2003) Neuroreport, 14, pp. 1815-1819; Price, D.T., Loscalzo, J., Cellular adhesion molecules and atherogenesis (1999) Am. J. Med, 107, pp. 85-97; O'Brien, K.D., Allen, M.D., McDonald, T.O., Chait, A., Vascular cell adhesion molecule-1 is expressed in human coronary atherosclerotic plaques. Implications for the mode of progression of advanced coronary atherosclerosis (1993) J. Clin. Invest, 92, pp. 945-951; Collins, T., Read, M.A., Neish, A.S., Whitley, M.Z., Transcriptional regulation of endothelial cell adhesion molecules: NF-kappa B and cytokine-inducible enhancers (1995) FASEB J, 9, pp. 899-909; Muslin, A.J., J., MAPK signalling in cardiovascular health and disease: Molecular mechanisms and therapeutic targets (2008) Clin. Sci, 115, pp. 203-218; Lin, S.J., Shyue, S.K., Hung, Y.Y., Chen, Y.H., Superoxide dismutase inhibits the expression of vascular cell adhesion molecule-1 and intracellular cell adhesion molecule-1 induced by tumor necrosis factor-a in human endothelial cells through the JNK/p38 pathways (2005) Arterioscler. Thromb. Vasc. Biol, 25, pp. 334-340; Brennan, C.M., Steitz, J.A., HuR and mRNA stability (2001) Cell. Mol. Life Sci, 58, pp. 266-277; Baud, V., Karin, M., Signal transduction by tumor necrosis factor and its relatives (2001) Trends Cell Biol, 11, pp. 372-377; Ghafoorunissa, H.S., Rao, M.V., Sesame lignans enhance antioxidant activity of vitamin E in lipid peroxidation systems (2004) Mol. Cell. Biochem, 262, pp. 195-202; Hsu, D.Z., Su, S.B., Chien, S.P., Chiang, P.J., Effect of sesame oil on oxidative-stress-associated renal injury in endotoxemic rats: Involvement of nitric oxide and proinflammatory cytokines (2005) Shock, 24, pp. 276-280; Nakano, D., Kurumazuka, D., Nagai, Y., Nishiyama, A., Dietary sesamin suppresses aortic NADPH oxidase in DOCA salt hypertensive rats (2008) Clin. Exp. Pharmacol. Physiol, 35, pp. 324-326; Hirose, N., Inoue, T., Nishihara, K., Sugano, M., Inhibition of cholesterol absorption and synthesis in rats by sesamin (1991) J. Lipid Res, 32, pp. 629-638; Kiso, Y., Tsuruoka, N., Kidokoro, A., Matsumoto, I., Abe, K., Sesamin ingestion regulates the transcription levels of hepatic metabolizing enzymes for alcohol and lipids in rats (2005) Alcohol Clin. Exp. Res, 29, pp. 116S-120S; Hou, R.C., Huang, H.M., Tzen, J.T., Jeng, K.C., Protective effects of sesamin and sesamolin on hypoxic neuronal and PC12 cells (2003) J. Neurosci. Res, 74, pp. 123-133; Wu, W.H., Kang, Y.P., Wang, N.H., Jou, H.J., Wang, T.A., Sesame ingestion affects sex hormones, antioxidant status, and blood lipids in postmenopausal women (2006) J. Nutr, 136, pp. 1270-1275; Wertheimer, S.J., Myers, C.L., Wallace, R.W., Parks, T.P., Intercellular adhesion molecule-1 gene expression in human endothelial cells. Differential regulation by tumor necrosis factor-alpha and phorbol myristate acetate (1992) J. Biol. Chem, 267, pp. 12030-12035; Nabors, B.L., Suswan, E., Huang, Y., Yang, X., Tumor necrosis factor alpha induces angiogenic factor upregulation in malignant glioma cells: A role for RNA stabilization and HuR (2003) Cancer Res, 63, pp. 4181-4187; Jeng, K.C., Hou, R.C., Wang, J.C., Ping, L.I., Sesamin inhibits lipopolysaccharide-induced cytokine production by suppression of p38 mitogen-activated protein kinase and nuclear factor-kappaB (2005) Immunol. Lett, 97, pp. 101-106; Hou, R.C., Wu, C.C., Yang, C.H., Jeng, K.C., Protective effects of sesamin and sesamolin on murine BV-2 microglia cell line under hypoxia (2004) Neurosci. Lett, 367, pp. 10-13; Ghosh, S., Baltimore, D., Activation in vitro of NF-kappa B by phosphorylation of its inhibitor I kappa B (1990) Nature, 344, pp. 678-682; Bhaskaran, S., Santanam, N., Penumetcha, M., Parthasarathy, S., Inhibition of atherosclerosis in low-density lipoprotein receptor-negative mice by sesame oil (2006) J. Med. Food, 9, pp. 487-490; Utsunomiya, T., Chavali, S.R., Zhong, W.W., Forse, R.A., Effects of sesamin-supplemented dietary fat emulsions on the ex vivo production of lipopolysaccharide-induced prostanoids and tumor necrosis factor alpha in rats (2000) Am. J. Clin. Nutr, 72, pp. 804-808; Joris, I., Zand, T., Nunnari, J.J., Krolikowski, F.J., Majno, G., Studies on the pathogenesis of atherosclerosis. I. Adhesion and emigration of mononuclear cells in the aorta of hypercholesterolemic rats (1983) Am. J. Pathol, 113, pp. 341-358",
year = "2010",
doi = "10.1002/mnfr.200900271",
language = "English",
volume = "54",
pages = "1340--1350",
journal = "Molecular Nutrition and Food Research",
issn = "1613-4125",
publisher = "Wiley-VCH Verlag",
number = "9",

}

TY - JOUR

T1 - Sesamin attenuates intercellular cell adhesion molecule-1 expression in vitro in TNF-α-treated human aortic endothelial cells and in vivo in apolipoprotein-E-deficient mice

AU - Wu, Wen-Huey

AU - Wang, Shu-Huei

AU - Kuan, I.-I.

AU - Kao, Ya-S.

AU - Wu, Pei-Jhen

AU - Liang, Chan-Jung

AU - Chien, Hsiung-Fei

AU - Kao, Chiu H.

AU - Huang, Ching-Jang

AU - Chen, Yuh-Lien

N1 - 被引用次數:10 Export Date: 16 March 2016 通訊地址: Chen, Y.-L.; Department of Anatomy and Cell Biology, College of Medicine, National Taiwan University, No. 1, Section 1, Ren-Ai Rd., Taipei 100, Taiwan; 電子郵件: ylchenv@ntu.edu.tw 化學物質/CAS: intercellular adhesion molecule 1, 126547-89-5; sesamin, 607-80-7, 7076-24-6; sesamol, 533-31-3; Anti-Inflammatory Agents, Non-Steroidal; Antigens, Surface; Antioxidants; Apolipoproteins E; Benzodioxoles; Dioxoles; ELAV-like protein 1; Intercellular Adhesion Molecule-1, 126547-89-5; Lignans; Phenols; RNA, Messenger; RNA, Small Interfering; RNA-Binding Proteins; Tumor Necrosis Factor-alpha; sesamin, 607-80-7; sesamol, 533-31-3 參考文獻: Budowski, P., Markley, K.S., The chemical and physiological properties of sesame oil (1951) Chem. Rev, 48, pp. 125-151; Akimoto, K., Kitagawa, Y., Akamatsu, T., Hirose, N., Protective effects of sesamin against liver damage caused by alcohol or carbon tetrachloride in rodents (1993) Ann. Nutr. Metab, 37, pp. 218-224; Hirose, N., Doi, F., Ueki, T., Akazawa, K., Suppressive effect of sesamin against 7,12-dimethylbenz-anthracene induced rat mammary carcinogenesis (1992) Anticancer Res, 12, pp. 1259-1265; Chavali, S.R., Zhong, W.W., Forse, R.A., Dietary alphalinolenic acid increases TNF-alpha, and decreases IL-6, IL-10 in response to LPS: Effects of sesamin on the delta-5 desaturation of o6 and o3 fatty acids in mice (1998) Essent. Fatty Acids, 58, pp. 185-191. , Prostaglandins Leuko; Chavali, S.R., Utsunomiya, T., Forse, R.A., Increased survival after cecal ligation and puncture in mice consuming diets enriched with sesame seed oil (2001) Crit. Care Med, 29, pp. 140-143; Huo, R.C., Chen, H.L., Tzen, J.T., Jeng, K.C., Effect of sesame antioxidants on LPS-induced NO production by BV2 microglial cells (2003) Neuroreport, 14, pp. 1815-1819; Price, D.T., Loscalzo, J., Cellular adhesion molecules and atherogenesis (1999) Am. J. Med, 107, pp. 85-97; O'Brien, K.D., Allen, M.D., McDonald, T.O., Chait, A., Vascular cell adhesion molecule-1 is expressed in human coronary atherosclerotic plaques. Implications for the mode of progression of advanced coronary atherosclerosis (1993) J. Clin. Invest, 92, pp. 945-951; Collins, T., Read, M.A., Neish, A.S., Whitley, M.Z., Transcriptional regulation of endothelial cell adhesion molecules: NF-kappa B and cytokine-inducible enhancers (1995) FASEB J, 9, pp. 899-909; Muslin, A.J., J., MAPK signalling in cardiovascular health and disease: Molecular mechanisms and therapeutic targets (2008) Clin. Sci, 115, pp. 203-218; Lin, S.J., Shyue, S.K., Hung, Y.Y., Chen, Y.H., Superoxide dismutase inhibits the expression of vascular cell adhesion molecule-1 and intracellular cell adhesion molecule-1 induced by tumor necrosis factor-a in human endothelial cells through the JNK/p38 pathways (2005) Arterioscler. Thromb. Vasc. Biol, 25, pp. 334-340; Brennan, C.M., Steitz, J.A., HuR and mRNA stability (2001) Cell. Mol. Life Sci, 58, pp. 266-277; Baud, V., Karin, M., Signal transduction by tumor necrosis factor and its relatives (2001) Trends Cell Biol, 11, pp. 372-377; Ghafoorunissa, H.S., Rao, M.V., Sesame lignans enhance antioxidant activity of vitamin E in lipid peroxidation systems (2004) Mol. Cell. Biochem, 262, pp. 195-202; Hsu, D.Z., Su, S.B., Chien, S.P., Chiang, P.J., Effect of sesame oil on oxidative-stress-associated renal injury in endotoxemic rats: Involvement of nitric oxide and proinflammatory cytokines (2005) Shock, 24, pp. 276-280; Nakano, D., Kurumazuka, D., Nagai, Y., Nishiyama, A., Dietary sesamin suppresses aortic NADPH oxidase in DOCA salt hypertensive rats (2008) Clin. Exp. Pharmacol. Physiol, 35, pp. 324-326; Hirose, N., Inoue, T., Nishihara, K., Sugano, M., Inhibition of cholesterol absorption and synthesis in rats by sesamin (1991) J. Lipid Res, 32, pp. 629-638; Kiso, Y., Tsuruoka, N., Kidokoro, A., Matsumoto, I., Abe, K., Sesamin ingestion regulates the transcription levels of hepatic metabolizing enzymes for alcohol and lipids in rats (2005) Alcohol Clin. Exp. Res, 29, pp. 116S-120S; Hou, R.C., Huang, H.M., Tzen, J.T., Jeng, K.C., Protective effects of sesamin and sesamolin on hypoxic neuronal and PC12 cells (2003) J. Neurosci. Res, 74, pp. 123-133; Wu, W.H., Kang, Y.P., Wang, N.H., Jou, H.J., Wang, T.A., Sesame ingestion affects sex hormones, antioxidant status, and blood lipids in postmenopausal women (2006) J. Nutr, 136, pp. 1270-1275; Wertheimer, S.J., Myers, C.L., Wallace, R.W., Parks, T.P., Intercellular adhesion molecule-1 gene expression in human endothelial cells. Differential regulation by tumor necrosis factor-alpha and phorbol myristate acetate (1992) J. Biol. Chem, 267, pp. 12030-12035; Nabors, B.L., Suswan, E., Huang, Y., Yang, X., Tumor necrosis factor alpha induces angiogenic factor upregulation in malignant glioma cells: A role for RNA stabilization and HuR (2003) Cancer Res, 63, pp. 4181-4187; Jeng, K.C., Hou, R.C., Wang, J.C., Ping, L.I., Sesamin inhibits lipopolysaccharide-induced cytokine production by suppression of p38 mitogen-activated protein kinase and nuclear factor-kappaB (2005) Immunol. Lett, 97, pp. 101-106; Hou, R.C., Wu, C.C., Yang, C.H., Jeng, K.C., Protective effects of sesamin and sesamolin on murine BV-2 microglia cell line under hypoxia (2004) Neurosci. Lett, 367, pp. 10-13; Ghosh, S., Baltimore, D., Activation in vitro of NF-kappa B by phosphorylation of its inhibitor I kappa B (1990) Nature, 344, pp. 678-682; Bhaskaran, S., Santanam, N., Penumetcha, M., Parthasarathy, S., Inhibition of atherosclerosis in low-density lipoprotein receptor-negative mice by sesame oil (2006) J. Med. Food, 9, pp. 487-490; Utsunomiya, T., Chavali, S.R., Zhong, W.W., Forse, R.A., Effects of sesamin-supplemented dietary fat emulsions on the ex vivo production of lipopolysaccharide-induced prostanoids and tumor necrosis factor alpha in rats (2000) Am. J. Clin. Nutr, 72, pp. 804-808; Joris, I., Zand, T., Nunnari, J.J., Krolikowski, F.J., Majno, G., Studies on the pathogenesis of atherosclerosis. I. Adhesion and emigration of mononuclear cells in the aorta of hypercholesterolemic rats (1983) Am. J. Pathol, 113, pp. 341-358

PY - 2010

Y1 - 2010

N2 - Sesame lignans have antioxidative and anti-inflammatory properties. We focused on the effects of the lignans sesamin and sesamol on the expression of endothelial-leukocyte adhesion molecules in tumor necrosis factor-α (TNF-α)-treated human aortic endothelial cells (HAECs). When HAECs were pretreated with sesamin (10 or 100 μM), the TNF-α-induced expression of intercellular cell adhesion molecule-1 (ICAM-1) was significantly reduced (35 or 70% decrease, respectively) by Western blotting. Sesamol was less effective at inhibiting ICAM-1 expression (30% decrease at 100 μM). Sesamin and sesamol reduced the marked TNF-α-induced increase in human antigen R (HuR) translocation and the interaction between HuR and the 3'UTR of ICAM-1 mRNA. Both significantly reduced the binding of monocytes to TNF-α-stimulated HAECs. Sesamin significantly attenuated TNF-α-induced ICAM-1 expression and cell adhesion by downregulation of extracellular signal-regulated kinase 1/2 and p38. Furthermore, in vivo, sesamin attenuated intimal thickening and ICAM-1 expression seen in aortas of apolipoprotein-E-deficient mice. Taken together, these data suggest that sesamin inhibits TNF-α -induced extracellular signal-regulated kinase/p38 phosphorylation, nuclear translocation of NF-κB p65, cytoplasmic translocalization of HuR and thereby suppresses ICAM-1 expression, resulting in reduced adhesion of leukocytes. These results also suggest that sesamin may prevent the development of atherosclerosis and inflammatory responses. © 2010 WILEY-VCH Verlag GmbH & Co.

AB - Sesame lignans have antioxidative and anti-inflammatory properties. We focused on the effects of the lignans sesamin and sesamol on the expression of endothelial-leukocyte adhesion molecules in tumor necrosis factor-α (TNF-α)-treated human aortic endothelial cells (HAECs). When HAECs were pretreated with sesamin (10 or 100 μM), the TNF-α-induced expression of intercellular cell adhesion molecule-1 (ICAM-1) was significantly reduced (35 or 70% decrease, respectively) by Western blotting. Sesamol was less effective at inhibiting ICAM-1 expression (30% decrease at 100 μM). Sesamin and sesamol reduced the marked TNF-α-induced increase in human antigen R (HuR) translocation and the interaction between HuR and the 3'UTR of ICAM-1 mRNA. Both significantly reduced the binding of monocytes to TNF-α-stimulated HAECs. Sesamin significantly attenuated TNF-α-induced ICAM-1 expression and cell adhesion by downregulation of extracellular signal-regulated kinase 1/2 and p38. Furthermore, in vivo, sesamin attenuated intimal thickening and ICAM-1 expression seen in aortas of apolipoprotein-E-deficient mice. Taken together, these data suggest that sesamin inhibits TNF-α -induced extracellular signal-regulated kinase/p38 phosphorylation, nuclear translocation of NF-κB p65, cytoplasmic translocalization of HuR and thereby suppresses ICAM-1 expression, resulting in reduced adhesion of leukocytes. These results also suggest that sesamin may prevent the development of atherosclerosis and inflammatory responses. © 2010 WILEY-VCH Verlag GmbH & Co.

KW - Atherosclerosis

KW - Endothelial cell

KW - Intercellular cell adhesion molecule-1

KW - NF-κb

KW - Sesamin

KW - 1,3 benzodioxole derivative

KW - 1,3 dioxolane derivative

KW - antioxidant

KW - apolipoprotein E

KW - ELAV-like protein 1

KW - HuR protein

KW - intercellular adhesion molecule 1

KW - lignan

KW - membrane antigen

KW - messenger RNA

KW - nonsteroid antiinflammatory agent

KW - phenol derivative

KW - RNA binding protein

KW - sesamin

KW - sesamol

KW - small interfering RNA

KW - tumor necrosis factor alpha

KW - animal

KW - aorta

KW - article

KW - atherosclerosis

KW - cell culture

KW - cell line

KW - comparative study

KW - cytology

KW - drug effect

KW - gene expression regulation

KW - genetics

KW - human

KW - metabolism

KW - monocyte

KW - mouse

KW - mouse mutant

KW - pathology

KW - randomization

KW - signal transduction

KW - vascular endothelium

KW - Animals

KW - Anti-Inflammatory Agents, Non-Steroidal

KW - Antigens, Surface

KW - Antioxidants

KW - Aorta

KW - Apolipoproteins E

KW - Benzodioxoles

KW - Cell Line

KW - Cells, Cultured

KW - Dioxoles

KW - Endothelium, Vascular

KW - Gene Expression Regulation

KW - Humans

KW - Intercellular Adhesion Molecule-1

KW - Lignans

KW - Mice

KW - Mice, Knockout

KW - Monocytes

KW - Phenols

KW - Random Allocation

KW - RNA, Messenger

KW - RNA, Small Interfering

KW - RNA-Binding Proteins

KW - Signal Transduction

KW - Tumor Necrosis Factor-alpha

KW - Mus

KW - Sesamum indicum

U2 - 10.1002/mnfr.200900271

DO - 10.1002/mnfr.200900271

M3 - Article

VL - 54

SP - 1340

EP - 1350

JO - Molecular Nutrition and Food Research

JF - Molecular Nutrition and Food Research

SN - 1613-4125

IS - 9

ER -