Abstract

Considerable evidence shows a key role for protein modification in the adverse effects of chemicals; however, the interaction of diesel exhaust particles (DEP) with proteins and the resulting biological activity remains unclear. DEP and carbon black (CB) suspensions with and without bovine serum albumin (BSA) were used to elucidate the biological effects of air pollutants. The DEP and CB samples were then divided into suspensions and supernatants. Two important goals of the interaction of DEP with BSA were as follows: (1) understanding BSA modification by particles and (2) investigating the effects of particles bound with BSA and the corresponding supernatants on cellular oxidative stress and inflammation. We observed significant free amino groups production was caused by DEP. Using liquid chromatography-mass spectrometry (LC-MS), we observed that BSA was significantly oxidised by DEP in the supernatants and that the peptides ETYGDMADCCEK, MPCTEDYLSLILNR and TVMENFVAFVDK, derived BSA-DEP conjugates, were also oxidised. In A549 cells, DEP-BSA suspensions and the corresponding supernatants reduced 8-hydroxy-2′-deoxyguanosine (8-OHdG) production and increased interleukin-6 (IL-6) levels when compared to DEP solutions without BSA. Our findings suggest that oxidatively modified forms of BSA caused by DEP could lead to oxidative stress and the activation of inflammation.

Original languageEnglish
Pages (from-to)385-393
Number of pages9
JournalChemico-Biological Interactions
Volume206
Issue number2
DOIs
Publication statusPublished - 2013

Fingerprint

Vehicle Emissions
Oxidative stress
Bovine Serum Albumin
Blood Proteins
Oxidative Stress
Oxidation
Soot
Suspensions
In Vitro Techniques
Inflammation
Air Pollutants
Liquid chromatography
Bioactivity
Liquid Chromatography
Mass spectrometry
Interleukin-6
Mass Spectrometry
Proteins
Chemical activation

Keywords

  • Amino acid
  • Bovine serum albumin
  • Inflammation
  • Oxidative stress
  • Peptide sequence
  • Proteomics

ASJC Scopus subject areas

  • Toxicology

Cite this

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title = "Serum protein oxidation by diesel exhaust particles: Effects on oxidative stress and inflammatory response in vitro",
abstract = "Considerable evidence shows a key role for protein modification in the adverse effects of chemicals; however, the interaction of diesel exhaust particles (DEP) with proteins and the resulting biological activity remains unclear. DEP and carbon black (CB) suspensions with and without bovine serum albumin (BSA) were used to elucidate the biological effects of air pollutants. The DEP and CB samples were then divided into suspensions and supernatants. Two important goals of the interaction of DEP with BSA were as follows: (1) understanding BSA modification by particles and (2) investigating the effects of particles bound with BSA and the corresponding supernatants on cellular oxidative stress and inflammation. We observed significant free amino groups production was caused by DEP. Using liquid chromatography-mass spectrometry (LC-MS), we observed that BSA was significantly oxidised by DEP in the supernatants and that the peptides ETYGDMADCCEK, MPCTEDYLSLILNR and TVMENFVAFVDK, derived BSA-DEP conjugates, were also oxidised. In A549 cells, DEP-BSA suspensions and the corresponding supernatants reduced 8-hydroxy-2′-deoxyguanosine (8-OHdG) production and increased interleukin-6 (IL-6) levels when compared to DEP solutions without BSA. Our findings suggest that oxidatively modified forms of BSA caused by DEP could lead to oxidative stress and the activation of inflammation.",
keywords = "Amino acid, Bovine serum albumin, Inflammation, Oxidative stress, Peptide sequence, Proteomics",
author = "Chiang, {Ling Ling} and Chen, {Hao Cheng} and Chun-Nin Lee and Chuang, {Kai Jen} and Chen, {Tzu Tao} and Chi-Tai Yeh and Wang, {Liang Shun} and Lee, {Wei Hua} and Lin, {Lian Yu} and Tseng, {Hsiu Er} and Chuang, {Hsiao Chi}",
year = "2013",
doi = "10.1016/j.cbi.2013.10.013",
language = "English",
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pages = "385--393",
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TY - JOUR

T1 - Serum protein oxidation by diesel exhaust particles

T2 - Effects on oxidative stress and inflammatory response in vitro

AU - Chiang, Ling Ling

AU - Chen, Hao Cheng

AU - Lee, Chun-Nin

AU - Chuang, Kai Jen

AU - Chen, Tzu Tao

AU - Yeh, Chi-Tai

AU - Wang, Liang Shun

AU - Lee, Wei Hua

AU - Lin, Lian Yu

AU - Tseng, Hsiu Er

AU - Chuang, Hsiao Chi

PY - 2013

Y1 - 2013

N2 - Considerable evidence shows a key role for protein modification in the adverse effects of chemicals; however, the interaction of diesel exhaust particles (DEP) with proteins and the resulting biological activity remains unclear. DEP and carbon black (CB) suspensions with and without bovine serum albumin (BSA) were used to elucidate the biological effects of air pollutants. The DEP and CB samples were then divided into suspensions and supernatants. Two important goals of the interaction of DEP with BSA were as follows: (1) understanding BSA modification by particles and (2) investigating the effects of particles bound with BSA and the corresponding supernatants on cellular oxidative stress and inflammation. We observed significant free amino groups production was caused by DEP. Using liquid chromatography-mass spectrometry (LC-MS), we observed that BSA was significantly oxidised by DEP in the supernatants and that the peptides ETYGDMADCCEK, MPCTEDYLSLILNR and TVMENFVAFVDK, derived BSA-DEP conjugates, were also oxidised. In A549 cells, DEP-BSA suspensions and the corresponding supernatants reduced 8-hydroxy-2′-deoxyguanosine (8-OHdG) production and increased interleukin-6 (IL-6) levels when compared to DEP solutions without BSA. Our findings suggest that oxidatively modified forms of BSA caused by DEP could lead to oxidative stress and the activation of inflammation.

AB - Considerable evidence shows a key role for protein modification in the adverse effects of chemicals; however, the interaction of diesel exhaust particles (DEP) with proteins and the resulting biological activity remains unclear. DEP and carbon black (CB) suspensions with and without bovine serum albumin (BSA) were used to elucidate the biological effects of air pollutants. The DEP and CB samples were then divided into suspensions and supernatants. Two important goals of the interaction of DEP with BSA were as follows: (1) understanding BSA modification by particles and (2) investigating the effects of particles bound with BSA and the corresponding supernatants on cellular oxidative stress and inflammation. We observed significant free amino groups production was caused by DEP. Using liquid chromatography-mass spectrometry (LC-MS), we observed that BSA was significantly oxidised by DEP in the supernatants and that the peptides ETYGDMADCCEK, MPCTEDYLSLILNR and TVMENFVAFVDK, derived BSA-DEP conjugates, were also oxidised. In A549 cells, DEP-BSA suspensions and the corresponding supernatants reduced 8-hydroxy-2′-deoxyguanosine (8-OHdG) production and increased interleukin-6 (IL-6) levels when compared to DEP solutions without BSA. Our findings suggest that oxidatively modified forms of BSA caused by DEP could lead to oxidative stress and the activation of inflammation.

KW - Amino acid

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KW - Oxidative stress

KW - Peptide sequence

KW - Proteomics

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