Abstract

Background: Chronic rhinosinusitis (CRS) is an inflammatory disease of the sinuses and mucosa with unclear pathogenesis. Interleukin (IL)-21 is mainly expressed in activated cluster of differentiation (CD)4= T cells and has potent regulatory effects on the immune system. Objective: This study is to determine whether IL-21 in the blood is correlated with CRS. Methods: The blood samples from CRS patients and normal controls were analyzed in correlation with clinical features. The eosinophil percentage was counted, and serum levels of total immunoglobulin E (IgE) and IL-21 were analyzed by enzyme-linked immunosorbent assay (ELISA). In addition, IL-21 and interferon (IFN)-= secreted from stimulated peripheral blood mononuclear cells (PBMCs) were measured by ELISA, and their mRNA expression levels were analyzed by real-time quantitative polymerase chain reaction (RT-qPCR). Disease severity was scored based on computed tomography (CT) scan, nasal endoscopy, and global osteitis scoring scale (GOSS). Results: A total of 55 CRS and 37 healthy subjects were recruited. The average levels of serum total IgE were 20 kU/L in normal group, 290 kU/L in CRS with nasal polys (CRSwNP), and 187 kU/L in CRS without nasal polys (CRSsNP). IL-21 levels were 28 pg/mL in normal group, 54 pg/mL in CRSwNP, and 71 pg/mL in CRSsNP. Both IgE and IL-21 were significantly elevated in both CRS patient subgroups. However, no significant difference was found between these two patient subgroups. The serum IL-21 levels correlated well with the disease severity in the patients. In addition, the secreted IL-21 was enhanced significantly in the patient's PBMCs stimulated by phytohemagglutin (PHA). Conclusion: IL-21 could be a target for diagnosis and treatment of CRS.

Original languageEnglish
Pages (from-to)e1-e6
JournalAmerican Journal of Rhinology and Allergy
Volume29
Issue number1
DOIs
Publication statusPublished - Jan 1 2015

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Serum
Nose
Immunoglobulin E
Blood Cells
Enzyme-Linked Immunosorbent Assay
interleukin-21
Osteitis
Eosinophils
Interferons
Endoscopy
Real-Time Polymerase Chain Reaction
Immune System
Healthy Volunteers
Mucous Membrane
Tomography
T-Lymphocytes
Messenger RNA
Therapeutics

ASJC Scopus subject areas

  • Immunology and Allergy
  • Otorhinolaryngology
  • Medicine(all)

Cite this

Serum level of interleukin-21 is elevated in chronic rhinosinusitis. / Chao, Pin Zhir; Hsieh, Ming-Shium; Lee, Fei Peng; Chen, Shao Yi; Cheng, Chao Wen; Chang, Hui Wen; Lin, Yun Tien; Ting, Lai Lei; Lin, Yung Feng; Chen, Chien Ho.

In: American Journal of Rhinology and Allergy, Vol. 29, No. 1, 01.01.2015, p. e1-e6.

Research output: Contribution to journalArticle

Chao, Pin Zhir ; Hsieh, Ming-Shium ; Lee, Fei Peng ; Chen, Shao Yi ; Cheng, Chao Wen ; Chang, Hui Wen ; Lin, Yun Tien ; Ting, Lai Lei ; Lin, Yung Feng ; Chen, Chien Ho. / Serum level of interleukin-21 is elevated in chronic rhinosinusitis. In: American Journal of Rhinology and Allergy. 2015 ; Vol. 29, No. 1. pp. e1-e6.
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abstract = "Background: Chronic rhinosinusitis (CRS) is an inflammatory disease of the sinuses and mucosa with unclear pathogenesis. Interleukin (IL)-21 is mainly expressed in activated cluster of differentiation (CD)4= T cells and has potent regulatory effects on the immune system. Objective: This study is to determine whether IL-21 in the blood is correlated with CRS. Methods: The blood samples from CRS patients and normal controls were analyzed in correlation with clinical features. The eosinophil percentage was counted, and serum levels of total immunoglobulin E (IgE) and IL-21 were analyzed by enzyme-linked immunosorbent assay (ELISA). In addition, IL-21 and interferon (IFN)-= secreted from stimulated peripheral blood mononuclear cells (PBMCs) were measured by ELISA, and their mRNA expression levels were analyzed by real-time quantitative polymerase chain reaction (RT-qPCR). Disease severity was scored based on computed tomography (CT) scan, nasal endoscopy, and global osteitis scoring scale (GOSS). Results: A total of 55 CRS and 37 healthy subjects were recruited. The average levels of serum total IgE were 20 kU/L in normal group, 290 kU/L in CRS with nasal polys (CRSwNP), and 187 kU/L in CRS without nasal polys (CRSsNP). IL-21 levels were 28 pg/mL in normal group, 54 pg/mL in CRSwNP, and 71 pg/mL in CRSsNP. Both IgE and IL-21 were significantly elevated in both CRS patient subgroups. However, no significant difference was found between these two patient subgroups. The serum IL-21 levels correlated well with the disease severity in the patients. In addition, the secreted IL-21 was enhanced significantly in the patient's PBMCs stimulated by phytohemagglutin (PHA). Conclusion: IL-21 could be a target for diagnosis and treatment of CRS.",
author = "Chao, {Pin Zhir} and Ming-Shium Hsieh and Lee, {Fei Peng} and Chen, {Shao Yi} and Cheng, {Chao Wen} and Chang, {Hui Wen} and Lin, {Yun Tien} and Ting, {Lai Lei} and Lin, {Yung Feng} and Chen, {Chien Ho}",
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T1 - Serum level of interleukin-21 is elevated in chronic rhinosinusitis

AU - Chao, Pin Zhir

AU - Hsieh, Ming-Shium

AU - Lee, Fei Peng

AU - Chen, Shao Yi

AU - Cheng, Chao Wen

AU - Chang, Hui Wen

AU - Lin, Yun Tien

AU - Ting, Lai Lei

AU - Lin, Yung Feng

AU - Chen, Chien Ho

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Background: Chronic rhinosinusitis (CRS) is an inflammatory disease of the sinuses and mucosa with unclear pathogenesis. Interleukin (IL)-21 is mainly expressed in activated cluster of differentiation (CD)4= T cells and has potent regulatory effects on the immune system. Objective: This study is to determine whether IL-21 in the blood is correlated with CRS. Methods: The blood samples from CRS patients and normal controls were analyzed in correlation with clinical features. The eosinophil percentage was counted, and serum levels of total immunoglobulin E (IgE) and IL-21 were analyzed by enzyme-linked immunosorbent assay (ELISA). In addition, IL-21 and interferon (IFN)-= secreted from stimulated peripheral blood mononuclear cells (PBMCs) were measured by ELISA, and their mRNA expression levels were analyzed by real-time quantitative polymerase chain reaction (RT-qPCR). Disease severity was scored based on computed tomography (CT) scan, nasal endoscopy, and global osteitis scoring scale (GOSS). Results: A total of 55 CRS and 37 healthy subjects were recruited. The average levels of serum total IgE were 20 kU/L in normal group, 290 kU/L in CRS with nasal polys (CRSwNP), and 187 kU/L in CRS without nasal polys (CRSsNP). IL-21 levels were 28 pg/mL in normal group, 54 pg/mL in CRSwNP, and 71 pg/mL in CRSsNP. Both IgE and IL-21 were significantly elevated in both CRS patient subgroups. However, no significant difference was found between these two patient subgroups. The serum IL-21 levels correlated well with the disease severity in the patients. In addition, the secreted IL-21 was enhanced significantly in the patient's PBMCs stimulated by phytohemagglutin (PHA). Conclusion: IL-21 could be a target for diagnosis and treatment of CRS.

AB - Background: Chronic rhinosinusitis (CRS) is an inflammatory disease of the sinuses and mucosa with unclear pathogenesis. Interleukin (IL)-21 is mainly expressed in activated cluster of differentiation (CD)4= T cells and has potent regulatory effects on the immune system. Objective: This study is to determine whether IL-21 in the blood is correlated with CRS. Methods: The blood samples from CRS patients and normal controls were analyzed in correlation with clinical features. The eosinophil percentage was counted, and serum levels of total immunoglobulin E (IgE) and IL-21 were analyzed by enzyme-linked immunosorbent assay (ELISA). In addition, IL-21 and interferon (IFN)-= secreted from stimulated peripheral blood mononuclear cells (PBMCs) were measured by ELISA, and their mRNA expression levels were analyzed by real-time quantitative polymerase chain reaction (RT-qPCR). Disease severity was scored based on computed tomography (CT) scan, nasal endoscopy, and global osteitis scoring scale (GOSS). Results: A total of 55 CRS and 37 healthy subjects were recruited. The average levels of serum total IgE were 20 kU/L in normal group, 290 kU/L in CRS with nasal polys (CRSwNP), and 187 kU/L in CRS without nasal polys (CRSsNP). IL-21 levels were 28 pg/mL in normal group, 54 pg/mL in CRSwNP, and 71 pg/mL in CRSsNP. Both IgE and IL-21 were significantly elevated in both CRS patient subgroups. However, no significant difference was found between these two patient subgroups. The serum IL-21 levels correlated well with the disease severity in the patients. In addition, the secreted IL-21 was enhanced significantly in the patient's PBMCs stimulated by phytohemagglutin (PHA). Conclusion: IL-21 could be a target for diagnosis and treatment of CRS.

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