Serotype-specific detection of enterovirus 71 in clinical specimens by DNA microchip array

Shin Ru Shih, Yih Weng Wang, Guang Wu Chen, Luan Yin Chang, Tzou Yien Lin, Mei Chen Tseng, Chiayn Chiang, Kuo Chien Tsao, Chung Guei Huang, Mei Ren Shio, Jui Hung Tai, Shin Hwan Wang, Rei Lin Kuo, Wu Tse Liu

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Enterovirus 71 is an important pathogen that causes high morbidity and mortality in children in Taiwan. Virus isolation in cell cultures has been the standard method for enterovirus 71 identification in Clinical Virology Laboratories. However, virus isolation takes 5-10 days when using cell culture. A microchip for enterovirus 71 detection was developed as an alternative diagnostic method. The novel approach is based on hybridization of amplified DNA specimens with oligonucleotide DNA probes immobilized on a microchip. Two oligonucletides were used as detection probes, the pan-enterovirus sequence located in the 5′-noncoding region (5′-NCR) and the enterovirus 71-specific sequence located in the VP2 region. The diagnostic procedure takes 6 h. One hundred specimens identified as enteroviruses by viral cultures were tested using this microchip, including 67 enterovirus 71 specimens. The sensitivity of the novel method is 89.6% and its specificity is 90.9%. The enterovirus 71-microchip can detect the amplicon derived from viral RNA corresponding to 1-10 virions in a clinical specimen. Microchip array is a potential diagnostic method for identification of enterovirus in the future.

Original languageEnglish
Pages (from-to)55-60
Number of pages6
JournalJournal of Virological Methods
Volume111
Issue number1
DOIs
Publication statusPublished - Jul 1 2003
Externally publishedYes

Keywords

  • Enterovirus 71
  • Microchip

ASJC Scopus subject areas

  • Virology

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    Shih, S. R., Wang, Y. W., Chen, G. W., Chang, L. Y., Lin, T. Y., Tseng, M. C., Chiang, C., Tsao, K. C., Huang, C. G., Shio, M. R., Tai, J. H., Wang, S. H., Kuo, R. L., & Liu, W. T. (2003). Serotype-specific detection of enterovirus 71 in clinical specimens by DNA microchip array. Journal of Virological Methods, 111(1), 55-60. https://doi.org/10.1016/S0166-0934(03)00151-4