Abstract
Clinical reports document that depression as a side effect is more prevalent in hepatic patients given interferon (IFN)-α therapy than in those given lamivudine. The mechanisms, however, are poorly understood. Serotonin transporter (5-HTT), via uptake of serotonin (5-HT) into presynaptic serotoninergic neurons, is an initial action site for antidepressants. Real-time polymerase chain reaction (PCR) was used to quantify 5-HTT mRNA expression in immune cells in order to evaluate whether 5-HTT acted as an indicator of depression. Results showed that the 5-HTT mRNA expression was much higher in T-cell and B-cell lines than that in a monocytic cell line. Treatment with either lamivudine or ribavirin reduced the 5-HTT mRNA expression, protein level and 5-HT uptake in T-cell line. Treatment with IFN-α, however, increased those levels in the same group. A similar effect was observed in peripheral blood mononuclear cells (PBMC). Mimicking clinical use by treating PBMC with a combination of IFN-α and ribavirin increased the 5-HTT mRNA expression level. Our study indicates that these therapeutic drugs regulate 5-HTT expression, which implies that 5-HTT might be a trait marker in IFN-α-induced depression after hepatic therapy.
| Original language | English |
|---|---|
| Pages (from-to) | 106-115 |
| Number of pages | 10 |
| Journal | Scandinavian Journal of Immunology |
| Volume | 63 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Feb 1 2006 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
Cite this
Serotonin transporter mRNA expression is decreased by lamivudine and ribavirin and increased by interferon in immune cells. / Tsao, C. W.; Lin, Y. S.; Cheng, J. T.; Chang, W. W.; Chen, C. L.; Wu, S. R.; Fan, C. W.; Lo, H. Y.
In: Scandinavian Journal of Immunology, Vol. 63, No. 2, 01.02.2006, p. 106-115.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Serotonin transporter mRNA expression is decreased by lamivudine and ribavirin and increased by interferon in immune cells
AU - Tsao, C. W.
AU - Lin, Y. S.
AU - Cheng, J. T.
AU - Chang, W. W.
AU - Chen, C. L.
AU - Wu, S. R.
AU - Fan, C. W.
AU - Lo, H. Y.
PY - 2006/2/1
Y1 - 2006/2/1
N2 - Clinical reports document that depression as a side effect is more prevalent in hepatic patients given interferon (IFN)-α therapy than in those given lamivudine. The mechanisms, however, are poorly understood. Serotonin transporter (5-HTT), via uptake of serotonin (5-HT) into presynaptic serotoninergic neurons, is an initial action site for antidepressants. Real-time polymerase chain reaction (PCR) was used to quantify 5-HTT mRNA expression in immune cells in order to evaluate whether 5-HTT acted as an indicator of depression. Results showed that the 5-HTT mRNA expression was much higher in T-cell and B-cell lines than that in a monocytic cell line. Treatment with either lamivudine or ribavirin reduced the 5-HTT mRNA expression, protein level and 5-HT uptake in T-cell line. Treatment with IFN-α, however, increased those levels in the same group. A similar effect was observed in peripheral blood mononuclear cells (PBMC). Mimicking clinical use by treating PBMC with a combination of IFN-α and ribavirin increased the 5-HTT mRNA expression level. Our study indicates that these therapeutic drugs regulate 5-HTT expression, which implies that 5-HTT might be a trait marker in IFN-α-induced depression after hepatic therapy.
AB - Clinical reports document that depression as a side effect is more prevalent in hepatic patients given interferon (IFN)-α therapy than in those given lamivudine. The mechanisms, however, are poorly understood. Serotonin transporter (5-HTT), via uptake of serotonin (5-HT) into presynaptic serotoninergic neurons, is an initial action site for antidepressants. Real-time polymerase chain reaction (PCR) was used to quantify 5-HTT mRNA expression in immune cells in order to evaluate whether 5-HTT acted as an indicator of depression. Results showed that the 5-HTT mRNA expression was much higher in T-cell and B-cell lines than that in a monocytic cell line. Treatment with either lamivudine or ribavirin reduced the 5-HTT mRNA expression, protein level and 5-HT uptake in T-cell line. Treatment with IFN-α, however, increased those levels in the same group. A similar effect was observed in peripheral blood mononuclear cells (PBMC). Mimicking clinical use by treating PBMC with a combination of IFN-α and ribavirin increased the 5-HTT mRNA expression level. Our study indicates that these therapeutic drugs regulate 5-HTT expression, which implies that 5-HTT might be a trait marker in IFN-α-induced depression after hepatic therapy.
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UR - http://www.scopus.com/inward/citedby.url?scp=33645060177&partnerID=8YFLogxK
U2 - 10.1111/j.1365-3083.2005.01715.x
DO - 10.1111/j.1365-3083.2005.01715.x
M3 - Article
VL - 63
SP - 106
EP - 115
JO - Scandinavian Journal of Immunology
JF - Scandinavian Journal of Immunology
SN - 0300-9475
IS - 2
ER -