Serine protease inhibitor (SERPIN) B1 promotes oral cancer cell motility and is over-expressed in invasive oral squamous cell carcinoma

Mei Yu Tseng, Shyun Yeu Liu, Hau Ren Chen, Yu Jen Wu, Chien Chih Chiu, Po Ting Chan, Wei Fan Chiang, Yu Chi Liu, Chien Yu Lu, Yuh Shan Jou, Jeff Yi Fu Chen

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Lymph node metastasis is the hallmark of malignant neoplasms in patients of oral cancer, accounting for the poor diagnosis and reduced 5-year survival rate. Here we sought to identify cell motility-associated proteins of oral cancer by proteomic approach. We compared the proteomes of two oral cancer cells, CAL-27 and SAS, with the highest and the lowest migration potential, respectively, amongst six different oral cancer cell lines. Subsequent identification of differentially expressed proteins by LC-MS/MS and Western analysis revealed that SERPINB1 (serine protease inhibitor, clade B, member1) was highly expressed in CAL-27, the high-motility oral cancer cells. Semi-quantitative and real-time PCR further confirmed differential expression of SERPINB1 in these two cell lines at mRNA level. To verify the motility-promoting function of SERPINB1 in oral cancer cells, we showed that endogenous expression of SERPINB1 correlated positively with cell migration. Moreover, ectopic expression of SERPINB1 in oral cancer cells, SAS, Ca9-22, CAL-27 and HSC-3, increased cell migration by 25%, 52%, 90% and 100%, respectively. Finally, we found that the expression of SERPINB1 was significantly higher in 5 of 8 (62.5%) oral cancer tissues compared with the matched adjacent normal tissues. Besides, immunohistochemical results indicated over-expression of SERPINB1 in clinicopathologically invasive oral squamous cell carcinoma (OSCC) but not in normal oral mucosa (p <0.01). Together, our findings have provided a possible biomarker for oral cancer metastasis.

Original languageEnglish
Pages (from-to)771-776
Number of pages6
JournalOral Oncology
Volume45
Issue number9
DOIs
Publication statusPublished - Sep 2009

Fingerprint

Serine Proteinase Inhibitors
Mouth Neoplasms
Cell Movement
Squamous Cell Carcinoma
Molecular Motor Proteins
Neoplasm Metastasis
Cell Line
Mouth Mucosa
Proteome
Proteomics
Real-Time Polymerase Chain Reaction
Survival Rate
Biomarkers
Lymph Nodes
Messenger RNA

Keywords

  • Invasive oral squamous cell carcinoma
  • Metastases
  • Motility
  • Oral cancer
  • SERPINB1

ASJC Scopus subject areas

  • Oncology
  • Oral Surgery
  • Cancer Research

Cite this

Serine protease inhibitor (SERPIN) B1 promotes oral cancer cell motility and is over-expressed in invasive oral squamous cell carcinoma. / Tseng, Mei Yu; Liu, Shyun Yeu; Chen, Hau Ren; Wu, Yu Jen; Chiu, Chien Chih; Chan, Po Ting; Chiang, Wei Fan; Liu, Yu Chi; Lu, Chien Yu; Jou, Yuh Shan; Chen, Jeff Yi Fu.

In: Oral Oncology, Vol. 45, No. 9, 09.2009, p. 771-776.

Research output: Contribution to journalArticle

Tseng, MY, Liu, SY, Chen, HR, Wu, YJ, Chiu, CC, Chan, PT, Chiang, WF, Liu, YC, Lu, CY, Jou, YS & Chen, JYF 2009, 'Serine protease inhibitor (SERPIN) B1 promotes oral cancer cell motility and is over-expressed in invasive oral squamous cell carcinoma', Oral Oncology, vol. 45, no. 9, pp. 771-776. https://doi.org/10.1016/j.oraloncology.2008.11.013
Tseng, Mei Yu ; Liu, Shyun Yeu ; Chen, Hau Ren ; Wu, Yu Jen ; Chiu, Chien Chih ; Chan, Po Ting ; Chiang, Wei Fan ; Liu, Yu Chi ; Lu, Chien Yu ; Jou, Yuh Shan ; Chen, Jeff Yi Fu. / Serine protease inhibitor (SERPIN) B1 promotes oral cancer cell motility and is over-expressed in invasive oral squamous cell carcinoma. In: Oral Oncology. 2009 ; Vol. 45, No. 9. pp. 771-776.
@article{2745f7e321414d13bed465139e6e8761,
title = "Serine protease inhibitor (SERPIN) B1 promotes oral cancer cell motility and is over-expressed in invasive oral squamous cell carcinoma",
abstract = "Lymph node metastasis is the hallmark of malignant neoplasms in patients of oral cancer, accounting for the poor diagnosis and reduced 5-year survival rate. Here we sought to identify cell motility-associated proteins of oral cancer by proteomic approach. We compared the proteomes of two oral cancer cells, CAL-27 and SAS, with the highest and the lowest migration potential, respectively, amongst six different oral cancer cell lines. Subsequent identification of differentially expressed proteins by LC-MS/MS and Western analysis revealed that SERPINB1 (serine protease inhibitor, clade B, member1) was highly expressed in CAL-27, the high-motility oral cancer cells. Semi-quantitative and real-time PCR further confirmed differential expression of SERPINB1 in these two cell lines at mRNA level. To verify the motility-promoting function of SERPINB1 in oral cancer cells, we showed that endogenous expression of SERPINB1 correlated positively with cell migration. Moreover, ectopic expression of SERPINB1 in oral cancer cells, SAS, Ca9-22, CAL-27 and HSC-3, increased cell migration by 25{\%}, 52{\%}, 90{\%} and 100{\%}, respectively. Finally, we found that the expression of SERPINB1 was significantly higher in 5 of 8 (62.5{\%}) oral cancer tissues compared with the matched adjacent normal tissues. Besides, immunohistochemical results indicated over-expression of SERPINB1 in clinicopathologically invasive oral squamous cell carcinoma (OSCC) but not in normal oral mucosa (p <0.01). Together, our findings have provided a possible biomarker for oral cancer metastasis.",
keywords = "Invasive oral squamous cell carcinoma, Metastases, Motility, Oral cancer, SERPINB1",
author = "Tseng, {Mei Yu} and Liu, {Shyun Yeu} and Chen, {Hau Ren} and Wu, {Yu Jen} and Chiu, {Chien Chih} and Chan, {Po Ting} and Chiang, {Wei Fan} and Liu, {Yu Chi} and Lu, {Chien Yu} and Jou, {Yuh Shan} and Chen, {Jeff Yi Fu}",
year = "2009",
month = "9",
doi = "10.1016/j.oraloncology.2008.11.013",
language = "English",
volume = "45",
pages = "771--776",
journal = "Oral Oncology",
issn = "1368-8375",
publisher = "Elsevier Limited",
number = "9",

}

TY - JOUR

T1 - Serine protease inhibitor (SERPIN) B1 promotes oral cancer cell motility and is over-expressed in invasive oral squamous cell carcinoma

AU - Tseng, Mei Yu

AU - Liu, Shyun Yeu

AU - Chen, Hau Ren

AU - Wu, Yu Jen

AU - Chiu, Chien Chih

AU - Chan, Po Ting

AU - Chiang, Wei Fan

AU - Liu, Yu Chi

AU - Lu, Chien Yu

AU - Jou, Yuh Shan

AU - Chen, Jeff Yi Fu

PY - 2009/9

Y1 - 2009/9

N2 - Lymph node metastasis is the hallmark of malignant neoplasms in patients of oral cancer, accounting for the poor diagnosis and reduced 5-year survival rate. Here we sought to identify cell motility-associated proteins of oral cancer by proteomic approach. We compared the proteomes of two oral cancer cells, CAL-27 and SAS, with the highest and the lowest migration potential, respectively, amongst six different oral cancer cell lines. Subsequent identification of differentially expressed proteins by LC-MS/MS and Western analysis revealed that SERPINB1 (serine protease inhibitor, clade B, member1) was highly expressed in CAL-27, the high-motility oral cancer cells. Semi-quantitative and real-time PCR further confirmed differential expression of SERPINB1 in these two cell lines at mRNA level. To verify the motility-promoting function of SERPINB1 in oral cancer cells, we showed that endogenous expression of SERPINB1 correlated positively with cell migration. Moreover, ectopic expression of SERPINB1 in oral cancer cells, SAS, Ca9-22, CAL-27 and HSC-3, increased cell migration by 25%, 52%, 90% and 100%, respectively. Finally, we found that the expression of SERPINB1 was significantly higher in 5 of 8 (62.5%) oral cancer tissues compared with the matched adjacent normal tissues. Besides, immunohistochemical results indicated over-expression of SERPINB1 in clinicopathologically invasive oral squamous cell carcinoma (OSCC) but not in normal oral mucosa (p <0.01). Together, our findings have provided a possible biomarker for oral cancer metastasis.

AB - Lymph node metastasis is the hallmark of malignant neoplasms in patients of oral cancer, accounting for the poor diagnosis and reduced 5-year survival rate. Here we sought to identify cell motility-associated proteins of oral cancer by proteomic approach. We compared the proteomes of two oral cancer cells, CAL-27 and SAS, with the highest and the lowest migration potential, respectively, amongst six different oral cancer cell lines. Subsequent identification of differentially expressed proteins by LC-MS/MS and Western analysis revealed that SERPINB1 (serine protease inhibitor, clade B, member1) was highly expressed in CAL-27, the high-motility oral cancer cells. Semi-quantitative and real-time PCR further confirmed differential expression of SERPINB1 in these two cell lines at mRNA level. To verify the motility-promoting function of SERPINB1 in oral cancer cells, we showed that endogenous expression of SERPINB1 correlated positively with cell migration. Moreover, ectopic expression of SERPINB1 in oral cancer cells, SAS, Ca9-22, CAL-27 and HSC-3, increased cell migration by 25%, 52%, 90% and 100%, respectively. Finally, we found that the expression of SERPINB1 was significantly higher in 5 of 8 (62.5%) oral cancer tissues compared with the matched adjacent normal tissues. Besides, immunohistochemical results indicated over-expression of SERPINB1 in clinicopathologically invasive oral squamous cell carcinoma (OSCC) but not in normal oral mucosa (p <0.01). Together, our findings have provided a possible biomarker for oral cancer metastasis.

KW - Invasive oral squamous cell carcinoma

KW - Metastases

KW - Motility

KW - Oral cancer

KW - SERPINB1

UR - http://www.scopus.com/inward/record.url?scp=69249236955&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=69249236955&partnerID=8YFLogxK

U2 - 10.1016/j.oraloncology.2008.11.013

DO - 10.1016/j.oraloncology.2008.11.013

M3 - Article

C2 - 19213596

AN - SCOPUS:69249236955

VL - 45

SP - 771

EP - 776

JO - Oral Oncology

JF - Oral Oncology

SN - 1368-8375

IS - 9

ER -