Sequential up-regulation of the c-fos, c-jun and bax genes in the cortex, striatum and cerebellum induced by a single injection of a low dose of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in C57BL/6 mice

Yi-Ru Chen, Ping Ching Hsu, Iee Lin Hsu, Geng Chang Yeh

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We investigated whether single injection of 1-methyl-4-phenyl-1,2,3,6- tetrahydropyridine (MPTP) (20 mg/kg) will alter the expression of pro-apoptotic genes, namely, the c-fos, c-jun, and bax, in the striatum, cortex, and cerebellum of adult male C57BL/6 mice using reverse transcription-polymerase chain reaction assay. Injection of MPTP induced a transient decrease in the content of tyrosine hydroxylase estimated by the immunoreactivity in the striatum, which completely recovered 14 day after injection. A rapid but transient up-regulation of c-fos and c-jun genes occurred an hour after MPTP-injection, and a delayed but persistent up-regulation of bax gene expression occurred 3 day after injection. The up-regulation of these genes was present in all the examined brain regions. This result suggests that MPTP, at a low dose causing transient degeneration in the striatum, is capable of triggering two genetic pathways related to the generation of apoptosis in both dopaminergic and non-dopaminergic systems in the mouse brain.

Original languageEnglish
Pages (from-to)49-52
Number of pages4
JournalNeuroscience Letters
Volume314
Issue number1-2
DOIs
Publication statusPublished - Nov 13 2001

Keywords

  • Apoptosis
  • Bax
  • C-fos
  • C-jun
  • Mouse brain
  • MPTP
  • Reverse transcription-polymerase chain reaction
  • Tyrosine hydroxylase

ASJC Scopus subject areas

  • Neuroscience(all)

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