Sequence Analyses and Antigenic Epitope Mapping of the Putative RNA-Directed RNA Polymerase of Five U.S. Bluetongue Viruses

I. JEN HUANG, GUANG YUH HWANG, YI YUAN YANG, EMIKO HAYAMA, JOSEPH K K LI

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We determined the complete nucleotide sequences of the cognate L1 double-stranded RNA segments of bluetongue virus (BTV) serotypes 2, 11, 13, and 17, which encode the putative RNA-directed RNA polymerase VP1. Each L1 gene contained 3944 nucleotides and was 10 bases shorter than the previously reported L1 gene of BTV 10. A single open reading frame which could encode the reported VP1 protein, 1302 amino acids in size, began with an initiation codon at nucleotides 12-14 and a termination codon at nucleotides 3918-3920. Analyses of the nucleotides of L1 genes and the deduced amino acid sequences of VP1 proteins of the five U. S. BTV serotypes indicated that the most recently isolated BTV-2 serotype from Florida was more distantly related than BTV-10, 11, 13, and 17, which were isolated primarily in the western U.S.A. The results are consistent with our hypothesis that BTVs-10, -11, -13, and -17 are derived from a single and common gene pool, and that BTV-2 belongs to a second, distinct gene pool. These genetic distinctions also reflected well with the known geographic distribution of the five U.S. BTV serotypes in North America. This putative RNA-directed RNA polymerase (149 KDa) was a basic protein, and the deduced amino acid sequences of the VP1 proteins contained seven highly conserved hydrophobic domains and many other sequence motifs which were also found in other known RNA polymerases. Four immunodominant but linear antigenic epitopes conserved among the VP1 of five U.S. BTVs were also been identified and mapped using monospecific oligoclonal antibodies.

Original languageEnglish
Pages (from-to)280-288
Number of pages9
JournalVirology
Volume214
Issue number1
DOIs
Publication statusPublished - Dec 1 1995

Fingerprint

Bluetongue virus
Epitope Mapping
RNA Replicase
Sequence Analysis
Nucleotides
Gene Pool
Amino Acid Sequence
Proteins
Genes
Initiator Codon
Double-Stranded RNA
Terminator Codon
DNA-Directed RNA Polymerases
North America
Open Reading Frames
Epitopes
Amino Acids
Serogroup
Antibodies

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Sequence Analyses and Antigenic Epitope Mapping of the Putative RNA-Directed RNA Polymerase of Five U.S. Bluetongue Viruses. / HUANG, I. JEN; HWANG, GUANG YUH; YANG, YI YUAN; HAYAMA, EMIKO; LI, JOSEPH K K.

In: Virology, Vol. 214, No. 1, 01.12.1995, p. 280-288.

Research output: Contribution to journalArticle

HUANG, I. JEN ; HWANG, GUANG YUH ; YANG, YI YUAN ; HAYAMA, EMIKO ; LI, JOSEPH K K. / Sequence Analyses and Antigenic Epitope Mapping of the Putative RNA-Directed RNA Polymerase of Five U.S. Bluetongue Viruses. In: Virology. 1995 ; Vol. 214, No. 1. pp. 280-288.
@article{48ed29377b7b471fbaf9fa4bcb834b08,
title = "Sequence Analyses and Antigenic Epitope Mapping of the Putative RNA-Directed RNA Polymerase of Five U.S. Bluetongue Viruses",
abstract = "We determined the complete nucleotide sequences of the cognate L1 double-stranded RNA segments of bluetongue virus (BTV) serotypes 2, 11, 13, and 17, which encode the putative RNA-directed RNA polymerase VP1. Each L1 gene contained 3944 nucleotides and was 10 bases shorter than the previously reported L1 gene of BTV 10. A single open reading frame which could encode the reported VP1 protein, 1302 amino acids in size, began with an initiation codon at nucleotides 12-14 and a termination codon at nucleotides 3918-3920. Analyses of the nucleotides of L1 genes and the deduced amino acid sequences of VP1 proteins of the five U. S. BTV serotypes indicated that the most recently isolated BTV-2 serotype from Florida was more distantly related than BTV-10, 11, 13, and 17, which were isolated primarily in the western U.S.A. The results are consistent with our hypothesis that BTVs-10, -11, -13, and -17 are derived from a single and common gene pool, and that BTV-2 belongs to a second, distinct gene pool. These genetic distinctions also reflected well with the known geographic distribution of the five U.S. BTV serotypes in North America. This putative RNA-directed RNA polymerase (149 KDa) was a basic protein, and the deduced amino acid sequences of the VP1 proteins contained seven highly conserved hydrophobic domains and many other sequence motifs which were also found in other known RNA polymerases. Four immunodominant but linear antigenic epitopes conserved among the VP1 of five U.S. BTVs were also been identified and mapped using monospecific oligoclonal antibodies.",
author = "HUANG, {I. JEN} and HWANG, {GUANG YUH} and YANG, {YI YUAN} and EMIKO HAYAMA and LI, {JOSEPH K K}",
year = "1995",
month = "12",
day = "1",
doi = "10.1006/viro.1995.9927",
language = "English",
volume = "214",
pages = "280--288",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Sequence Analyses and Antigenic Epitope Mapping of the Putative RNA-Directed RNA Polymerase of Five U.S. Bluetongue Viruses

AU - HUANG, I. JEN

AU - HWANG, GUANG YUH

AU - YANG, YI YUAN

AU - HAYAMA, EMIKO

AU - LI, JOSEPH K K

PY - 1995/12/1

Y1 - 1995/12/1

N2 - We determined the complete nucleotide sequences of the cognate L1 double-stranded RNA segments of bluetongue virus (BTV) serotypes 2, 11, 13, and 17, which encode the putative RNA-directed RNA polymerase VP1. Each L1 gene contained 3944 nucleotides and was 10 bases shorter than the previously reported L1 gene of BTV 10. A single open reading frame which could encode the reported VP1 protein, 1302 amino acids in size, began with an initiation codon at nucleotides 12-14 and a termination codon at nucleotides 3918-3920. Analyses of the nucleotides of L1 genes and the deduced amino acid sequences of VP1 proteins of the five U. S. BTV serotypes indicated that the most recently isolated BTV-2 serotype from Florida was more distantly related than BTV-10, 11, 13, and 17, which were isolated primarily in the western U.S.A. The results are consistent with our hypothesis that BTVs-10, -11, -13, and -17 are derived from a single and common gene pool, and that BTV-2 belongs to a second, distinct gene pool. These genetic distinctions also reflected well with the known geographic distribution of the five U.S. BTV serotypes in North America. This putative RNA-directed RNA polymerase (149 KDa) was a basic protein, and the deduced amino acid sequences of the VP1 proteins contained seven highly conserved hydrophobic domains and many other sequence motifs which were also found in other known RNA polymerases. Four immunodominant but linear antigenic epitopes conserved among the VP1 of five U.S. BTVs were also been identified and mapped using monospecific oligoclonal antibodies.

AB - We determined the complete nucleotide sequences of the cognate L1 double-stranded RNA segments of bluetongue virus (BTV) serotypes 2, 11, 13, and 17, which encode the putative RNA-directed RNA polymerase VP1. Each L1 gene contained 3944 nucleotides and was 10 bases shorter than the previously reported L1 gene of BTV 10. A single open reading frame which could encode the reported VP1 protein, 1302 amino acids in size, began with an initiation codon at nucleotides 12-14 and a termination codon at nucleotides 3918-3920. Analyses of the nucleotides of L1 genes and the deduced amino acid sequences of VP1 proteins of the five U. S. BTV serotypes indicated that the most recently isolated BTV-2 serotype from Florida was more distantly related than BTV-10, 11, 13, and 17, which were isolated primarily in the western U.S.A. The results are consistent with our hypothesis that BTVs-10, -11, -13, and -17 are derived from a single and common gene pool, and that BTV-2 belongs to a second, distinct gene pool. These genetic distinctions also reflected well with the known geographic distribution of the five U.S. BTV serotypes in North America. This putative RNA-directed RNA polymerase (149 KDa) was a basic protein, and the deduced amino acid sequences of the VP1 proteins contained seven highly conserved hydrophobic domains and many other sequence motifs which were also found in other known RNA polymerases. Four immunodominant but linear antigenic epitopes conserved among the VP1 of five U.S. BTVs were also been identified and mapped using monospecific oligoclonal antibodies.

UR - http://www.scopus.com/inward/record.url?scp=0028803061&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028803061&partnerID=8YFLogxK

U2 - 10.1006/viro.1995.9927

DO - 10.1006/viro.1995.9927

M3 - Article

C2 - 8525629

AN - SCOPUS:0028803061

VL - 214

SP - 280

EP - 288

JO - Virology

JF - Virology

SN - 0042-6822

IS - 1

ER -