Separation and structure determination of nicardipine photoproducts by LC-ESI-MS

Shih Ming Chen, Mau Chih Hsieh, Su Hui Chao, E-E Chang, Po Yu Wang, An-Bang Wu

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A sample of 0.104 M nicardipine in methanol was photoirradiated with a Philips 400 W UV lamp for 3 h in a photochemical chamber. A total of four major photoproducts were found from the HPLC chromatogram. The same sample was used for taking LC-MS, while eight major photoproducts were observed and the structures elucidated by analyzing the CID patterns of their respective mass spectra. A reaction scheme of nicardipine is proposed that the photochemical reactions occur mainly via oxidation of 1,4-dihydropyridine moiety, following the stepwise photo-reduction of the m-nitro group and demethylation of the ester group at 5-position of the pyridine ring.

Original languageEnglish
Pages (from-to)1008-1012
Number of pages5
JournalBiomedical Chromatography
Volume22
Issue number9
DOIs
Publication statusPublished - Sep 2008

Fingerprint

Nicardipine
Ultraviolet lamps
Photochemical reactions
Methanol
Esters
High Pressure Liquid Chromatography
Oxidation
pyridine
1,4-dihydropyridine

Keywords

  • HPLC
  • LC-MS
  • Nicardipine
  • Photoproducts

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Molecular Biology
  • Drug Discovery
  • Pharmacology

Cite this

Separation and structure determination of nicardipine photoproducts by LC-ESI-MS. / Chen, Shih Ming; Hsieh, Mau Chih; Chao, Su Hui; Chang, E-E; Wang, Po Yu; Wu, An-Bang.

In: Biomedical Chromatography, Vol. 22, No. 9, 09.2008, p. 1008-1012.

Research output: Contribution to journalArticle

Chen, SM, Hsieh, MC, Chao, SH, Chang, E-E, Wang, PY & Wu, A-B 2008, 'Separation and structure determination of nicardipine photoproducts by LC-ESI-MS', Biomedical Chromatography, vol. 22, no. 9, pp. 1008-1012. https://doi.org/10.1002/bmc.1020
Chen, Shih Ming ; Hsieh, Mau Chih ; Chao, Su Hui ; Chang, E-E ; Wang, Po Yu ; Wu, An-Bang. / Separation and structure determination of nicardipine photoproducts by LC-ESI-MS. In: Biomedical Chromatography. 2008 ; Vol. 22, No. 9. pp. 1008-1012.
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