Sensory transduction of pulmonary reactive oxygen species by capsaicin-sensitive vagal lung afferent fibres in rats

Ting Ruan, You Shuei Lin, Kae Shin Lin, Yu Ru Kou

Research output: Contribution to journalArticle

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Abstract

The mechanisms of sensory transduction of pulmonary reactive oxygen species (ROS) by capsaicin-sensitive vagal lung afferent fibres are unclear. To investigate the role of transient receptor potential vanilloid 1 (TRPV1) receptors and P2X purinoceptors in this sensory transduction, we recorded fibre activity (FA) from 132 fibres of this type in 132 anaesthetized and ventilated rats. Airway challenge of aerosolized H2O2 (0, 0.2 and 0.4%) produced a concentration-dependant fibre stimulation. The fibre responses to 0.4% H2O2 were attenuated by dimethylthiourea (a hydroxyl radical (·OH) scavenger; change in fibre activity (ΔFA), -55 ± 9%) or deferoxamine (an iron-chelator that prevents formation of ·OH; ΔFA, -59 ± 9%), were prevented by catalase (an enzyme catalysing H2O2; ΔFA, -96 ± 3%) and were unaffected by the vehicle for dimethylthiourea, iron-saturated deferoxamine or heat-inactivated catalase. The fibre responses to 0.4% H2O2 were attenuated by capsazepine (a TRPV1 receptor antagonist; ΔFA, -39 ± 9%) or iso-pyridoxalphosphate-6-azophenyl-2′,5′-disulphonate (iso-PPADS, a P2X receptor antagonist; ΔFA, -51 ± 9%), were further reduced by capsazepine and iso-PPADS in combination (ΔFA, -70 ± 13%), and were unaltered by their vehicles. The fibre responses to cigarette smoke (20 ml), an irritant that generates ROS, were attenuated by dimethylthiourea (ΔFA, -61 ± 9%) or capsazepine and iso-PPADS in combination (ΔFA, -67 ± 9%). These results suggest that both the TRPV1 and P2X receptors mediate the sensory transduction of ROS, especially H2O2 and ·OH, by capsaicin-sensitive vagal lung afferent fibres.

Original languageEnglish
Pages (from-to)563-578
Number of pages16
JournalJournal of Physiology
Volume565
Issue number2
DOIs
Publication statusPublished - Jun 1 2005

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Capsaicin
Reactive Oxygen Species
Deferoxamine
Catalase
Lung
Purinergic P2X Receptors
Iron
Irritants
Chelating Agents
Smoke
Tobacco Products
Hydroxyl Radical
Hot Temperature
Enzymes
capsazepine
pyridoxal phosphate-6-azophenyl-2',4'-disulfonic acid
vanilloid receptor subtype 1
1,3-dimethylthiourea

ASJC Scopus subject areas

  • Physiology

Cite this

Sensory transduction of pulmonary reactive oxygen species by capsaicin-sensitive vagal lung afferent fibres in rats. / Ruan, Ting; Lin, You Shuei; Lin, Kae Shin; Kou, Yu Ru.

In: Journal of Physiology, Vol. 565, No. 2, 01.06.2005, p. 563-578.

Research output: Contribution to journalArticle

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abstract = "The mechanisms of sensory transduction of pulmonary reactive oxygen species (ROS) by capsaicin-sensitive vagal lung afferent fibres are unclear. To investigate the role of transient receptor potential vanilloid 1 (TRPV1) receptors and P2X purinoceptors in this sensory transduction, we recorded fibre activity (FA) from 132 fibres of this type in 132 anaesthetized and ventilated rats. Airway challenge of aerosolized H2O2 (0, 0.2 and 0.4{\%}) produced a concentration-dependant fibre stimulation. The fibre responses to 0.4{\%} H2O2 were attenuated by dimethylthiourea (a hydroxyl radical (·OH) scavenger; change in fibre activity (ΔFA), -55 ± 9{\%}) or deferoxamine (an iron-chelator that prevents formation of ·OH; ΔFA, -59 ± 9{\%}), were prevented by catalase (an enzyme catalysing H2O2; ΔFA, -96 ± 3{\%}) and were unaffected by the vehicle for dimethylthiourea, iron-saturated deferoxamine or heat-inactivated catalase. The fibre responses to 0.4{\%} H2O2 were attenuated by capsazepine (a TRPV1 receptor antagonist; ΔFA, -39 ± 9{\%}) or iso-pyridoxalphosphate-6-azophenyl-2′,5′-disulphonate (iso-PPADS, a P2X receptor antagonist; ΔFA, -51 ± 9{\%}), were further reduced by capsazepine and iso-PPADS in combination (ΔFA, -70 ± 13{\%}), and were unaltered by their vehicles. The fibre responses to cigarette smoke (20 ml), an irritant that generates ROS, were attenuated by dimethylthiourea (ΔFA, -61 ± 9{\%}) or capsazepine and iso-PPADS in combination (ΔFA, -67 ± 9{\%}). These results suggest that both the TRPV1 and P2X receptors mediate the sensory transduction of ROS, especially H2O2 and ·OH, by capsaicin-sensitive vagal lung afferent fibres.",
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