Sensory and motor recovery after repairing transected cervical roots

Ming Chao Huang, Pei T. Chang, May Jywan Tsai, Huai Sheng Kuo, Wen Chun Kuo, Meng J. Lee, Ming J. Lo, I. H. Lee, Wen Cheng Huang, Liang Ming Lee, Yang Hsin Shih, Liang Shong Lee, Henrich Cheng

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Background: Adult mammal sensory axons avulsed through spinal dorsal root traction injuries, especially of the brachial plexus or cauda equina, cannot normally regenerate through axonal outgrowth from the DRG into the spinal cord, thus causing clinical conditions that require neuronal regeneration for sensory recovery and for which no successful treatment has yet been reported. Methods: To evaluate the sensory recovery of the forelimb after transection of their left cervical dorsal and ventral roots (C6-C8) at their spinal cord junctions, 22 SD rats were randomly assigned to 3 groups: transection only (control 1); transection followed by repair using intercostal nerve grafts and fibrin glue (control 2); transection, repair, and application of aFGF and fibrin glue (experimental group). The following tests were reperformed after retransecting the repaired nerve roots to discount collateral innervation from adjacent nerve roots: motor function (grasping power), mechanical sensitivity to pain and touch (foot-withdrawal response to mechanical stimuli), temperature sensitivity (foot-withdrawal response to cold stimulus), and electrophysiologic sensory responses (measurement of cortical SEP). Results: After transection and repair, the experimental group rats showed recovery in both motor (grasping power) and sensory (touch, pain, and temperature sensation) nerve functions. Neuronal regeneration was confirmed by the reappearance of cortical SEP and by its disappearance after retransection of the repaired cervical nerve roots. Conclusion: Using our strategy for repairing transected cervical nerve roots, motor and sensory recovery was achieved in adult rats. The success of our study highlights possible treatment options for humans with avulsion injuries of the dorsal roots from the spinal cord.

Original languageEnglish
JournalSurgical Neurology
Volume68
Issue number5 SUPPL.
DOIs
Publication statusPublished - Nov 2007

Fingerprint

Spinal Nerve Roots
Spinal Cord
Fibrin Tissue Adhesive
Touch
Foot
Regeneration
Intercostal Nerves
Cauda Equina
Pain
Temperature
Forelimb
Brachial Plexus
Diagnosis-Related Groups
Wounds and Injuries
Traction
Axons
Mammals
Transplants
Therapeutics
Power (Psychology)

Keywords

  • Cervical root
  • Cholerotoxin B-horseradish peroxidase retrograde axonal labeling
  • Regeneration
  • Somatosensory-evoked potential

ASJC Scopus subject areas

  • Clinical Neurology
  • Surgery

Cite this

Huang, M. C., Chang, P. T., Tsai, M. J., Kuo, H. S., Kuo, W. C., Lee, M. J., ... Cheng, H. (2007). Sensory and motor recovery after repairing transected cervical roots. Surgical Neurology, 68(5 SUPPL.). https://doi.org/10.1016/j.surneu.2006.09.031

Sensory and motor recovery after repairing transected cervical roots. / Huang, Ming Chao; Chang, Pei T.; Tsai, May Jywan; Kuo, Huai Sheng; Kuo, Wen Chun; Lee, Meng J.; Lo, Ming J.; Lee, I. H.; Huang, Wen Cheng; Lee, Liang Ming; Shih, Yang Hsin; Lee, Liang Shong; Cheng, Henrich.

In: Surgical Neurology, Vol. 68, No. 5 SUPPL., 11.2007.

Research output: Contribution to journalArticle

Huang, MC, Chang, PT, Tsai, MJ, Kuo, HS, Kuo, WC, Lee, MJ, Lo, MJ, Lee, IH, Huang, WC, Lee, LM, Shih, YH, Lee, LS & Cheng, H 2007, 'Sensory and motor recovery after repairing transected cervical roots', Surgical Neurology, vol. 68, no. 5 SUPPL.. https://doi.org/10.1016/j.surneu.2006.09.031
Huang, Ming Chao ; Chang, Pei T. ; Tsai, May Jywan ; Kuo, Huai Sheng ; Kuo, Wen Chun ; Lee, Meng J. ; Lo, Ming J. ; Lee, I. H. ; Huang, Wen Cheng ; Lee, Liang Ming ; Shih, Yang Hsin ; Lee, Liang Shong ; Cheng, Henrich. / Sensory and motor recovery after repairing transected cervical roots. In: Surgical Neurology. 2007 ; Vol. 68, No. 5 SUPPL.
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abstract = "Background: Adult mammal sensory axons avulsed through spinal dorsal root traction injuries, especially of the brachial plexus or cauda equina, cannot normally regenerate through axonal outgrowth from the DRG into the spinal cord, thus causing clinical conditions that require neuronal regeneration for sensory recovery and for which no successful treatment has yet been reported. Methods: To evaluate the sensory recovery of the forelimb after transection of their left cervical dorsal and ventral roots (C6-C8) at their spinal cord junctions, 22 SD rats were randomly assigned to 3 groups: transection only (control 1); transection followed by repair using intercostal nerve grafts and fibrin glue (control 2); transection, repair, and application of aFGF and fibrin glue (experimental group). The following tests were reperformed after retransecting the repaired nerve roots to discount collateral innervation from adjacent nerve roots: motor function (grasping power), mechanical sensitivity to pain and touch (foot-withdrawal response to mechanical stimuli), temperature sensitivity (foot-withdrawal response to cold stimulus), and electrophysiologic sensory responses (measurement of cortical SEP). Results: After transection and repair, the experimental group rats showed recovery in both motor (grasping power) and sensory (touch, pain, and temperature sensation) nerve functions. Neuronal regeneration was confirmed by the reappearance of cortical SEP and by its disappearance after retransection of the repaired cervical nerve roots. Conclusion: Using our strategy for repairing transected cervical nerve roots, motor and sensory recovery was achieved in adult rats. The success of our study highlights possible treatment options for humans with avulsion injuries of the dorsal roots from the spinal cord.",
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AU - Chang, Pei T.

AU - Tsai, May Jywan

AU - Kuo, Huai Sheng

AU - Kuo, Wen Chun

AU - Lee, Meng J.

AU - Lo, Ming J.

AU - Lee, I. H.

AU - Huang, Wen Cheng

AU - Lee, Liang Ming

AU - Shih, Yang Hsin

AU - Lee, Liang Shong

AU - Cheng, Henrich

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N2 - Background: Adult mammal sensory axons avulsed through spinal dorsal root traction injuries, especially of the brachial plexus or cauda equina, cannot normally regenerate through axonal outgrowth from the DRG into the spinal cord, thus causing clinical conditions that require neuronal regeneration for sensory recovery and for which no successful treatment has yet been reported. Methods: To evaluate the sensory recovery of the forelimb after transection of their left cervical dorsal and ventral roots (C6-C8) at their spinal cord junctions, 22 SD rats were randomly assigned to 3 groups: transection only (control 1); transection followed by repair using intercostal nerve grafts and fibrin glue (control 2); transection, repair, and application of aFGF and fibrin glue (experimental group). The following tests were reperformed after retransecting the repaired nerve roots to discount collateral innervation from adjacent nerve roots: motor function (grasping power), mechanical sensitivity to pain and touch (foot-withdrawal response to mechanical stimuli), temperature sensitivity (foot-withdrawal response to cold stimulus), and electrophysiologic sensory responses (measurement of cortical SEP). Results: After transection and repair, the experimental group rats showed recovery in both motor (grasping power) and sensory (touch, pain, and temperature sensation) nerve functions. Neuronal regeneration was confirmed by the reappearance of cortical SEP and by its disappearance after retransection of the repaired cervical nerve roots. Conclusion: Using our strategy for repairing transected cervical nerve roots, motor and sensory recovery was achieved in adult rats. The success of our study highlights possible treatment options for humans with avulsion injuries of the dorsal roots from the spinal cord.

AB - Background: Adult mammal sensory axons avulsed through spinal dorsal root traction injuries, especially of the brachial plexus or cauda equina, cannot normally regenerate through axonal outgrowth from the DRG into the spinal cord, thus causing clinical conditions that require neuronal regeneration for sensory recovery and for which no successful treatment has yet been reported. Methods: To evaluate the sensory recovery of the forelimb after transection of their left cervical dorsal and ventral roots (C6-C8) at their spinal cord junctions, 22 SD rats were randomly assigned to 3 groups: transection only (control 1); transection followed by repair using intercostal nerve grafts and fibrin glue (control 2); transection, repair, and application of aFGF and fibrin glue (experimental group). The following tests were reperformed after retransecting the repaired nerve roots to discount collateral innervation from adjacent nerve roots: motor function (grasping power), mechanical sensitivity to pain and touch (foot-withdrawal response to mechanical stimuli), temperature sensitivity (foot-withdrawal response to cold stimulus), and electrophysiologic sensory responses (measurement of cortical SEP). Results: After transection and repair, the experimental group rats showed recovery in both motor (grasping power) and sensory (touch, pain, and temperature sensation) nerve functions. Neuronal regeneration was confirmed by the reappearance of cortical SEP and by its disappearance after retransection of the repaired cervical nerve roots. Conclusion: Using our strategy for repairing transected cervical nerve roots, motor and sensory recovery was achieved in adult rats. The success of our study highlights possible treatment options for humans with avulsion injuries of the dorsal roots from the spinal cord.

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KW - Somatosensory-evoked potential

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