Semi-continuous bacterial cellulose production in a rotating disk bioreactor and its materials properties analysis

Shin Ping Lin, Shu Chen Hsieh, Kuan I. Chen, Ali Demirci, Kuan Chen Cheng

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A rotating disk bioreactor with plastic composite support (PCS) as the solid support was evaluated for bacterial cellulose (BCel) production. Results demonstrated that BCel can be produced in a semi-continuous manner. The BCel productivity reached around 0.24 g/L/day and can be sustained for at least five consecutive runs. Scanning electron microscopy results confirmed that Gluconacetobacter can attach on the PCS surface, which eliminates the need of reinoculation. X-ray diffraction patterns and mechanical analysis of BCel produced from this semi-continuous process exhibited lower crystallinity (66.9 %) and mechanical property (Young's modulus of 372.5 MPa) when compared with the BCel obtained from static culture (crystallinity = 88.7 %, Young's modulus of 3,955.6 MPa). Both BCel samples possessed similar water content (98.66 vs. 99.04 %) and thermostability (around 346 °C). In conclusion, the PCS rotating disk bioreactor system can be used to produce BCel in pellicle form with enhanced productivity and, meanwhile, can be scaled up easily to meet commercial need.

Original languageEnglish
Pages (from-to)835-844
Number of pages10
JournalCellulose
Volume21
Issue number1
DOIs
Publication statusPublished - Feb 1 2014
Externally publishedYes

Fingerprint

Rotating disks
Bioreactors
Cellulose
Materials properties
Plastics
Composite materials
Elastic moduli
Productivity
Diffraction patterns
Water content
X ray diffraction
Mechanical properties
Scanning electron microscopy

Keywords

  • Bacterial cellulose
  • Bioreactor design
  • Gluconacetobacter xylinum
  • Materials property analysis
  • Plastic composite support
  • Semi-continuous production

ASJC Scopus subject areas

  • Polymers and Plastics

Cite this

Semi-continuous bacterial cellulose production in a rotating disk bioreactor and its materials properties analysis. / Lin, Shin Ping; Hsieh, Shu Chen; Chen, Kuan I.; Demirci, Ali; Cheng, Kuan Chen.

In: Cellulose, Vol. 21, No. 1, 01.02.2014, p. 835-844.

Research output: Contribution to journalArticle

Lin, Shin Ping ; Hsieh, Shu Chen ; Chen, Kuan I. ; Demirci, Ali ; Cheng, Kuan Chen. / Semi-continuous bacterial cellulose production in a rotating disk bioreactor and its materials properties analysis. In: Cellulose. 2014 ; Vol. 21, No. 1. pp. 835-844.
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