Self-focusing Au@SiO2 nanorods with rhodamine 6G as highly sensitive SERS substrate for carcinoembryonic antigen detection

Tran Thi Bich Quyen, Chun Chao Chang, Wei Nien Su, Yih Huei Uen, Chun Jern Pan, Jyong Yue Liu, John Rick, Kai Yuan Lin, Bing Joe Hwang

Research output: Contribution to journalArticle

50 Citations (Scopus)


A highly sensitive self-focusing surface-enhanced Raman scattering (SERS) methodology has been developed using Au@SiO2 core-shell nanorods for carcinoembryonic antigen (CEA) detection. The SERS enhancement factor was evaluated for anisotropic Au@SiO2 nanorods with silica shells of various thicknesses, upon which Rhodamine 6G (R6G) dye was applied as a reporter molecule for the quantitative determination of CEA. The highest R6G signal was attained with a silica layer of 1-2 nm thickness. The self-focusing character originates from the antibody-antigen interaction, which facilitates the SERS probes assembly and significantly increases the detection sensitivity of the CEA. Our results show that the SERS technique is able to detect CEA within a wide concentration range. With an extremely low limit of detection (LOD) of 0.86 fg mL-1, the Au@SiO2 nanoprobes potentially enable the early diagnosis of cancer. Our work offers a low-cost route to the fabrication of sensing devices able to be used for monitoring cancer progression in natural matrices, such as blood.

Original languageEnglish
Pages (from-to)629-636
Number of pages8
JournalJournal of Materials Chemistry B
Issue number6
Publication statusPublished - Feb 14 2014


ASJC Scopus subject areas

  • Chemistry(all)
  • Biomedical Engineering
  • Medicine(all)
  • Materials Science(all)

Cite this