16 Citations (Scopus)

Abstract

Successful oral delivery of therapeutic proteins such as insulin can greatly improve the quality of life of patients. This study develops a bubble carrier system by loading diethylene triamine pentaacetic acid (DTPA) dianhydride, a foaming agent (sodium bicarbonate; SBC), a surfactant (sodium dodecyl sulfate; SDS), and a protein drug (insulin) in an enteric-coated gelatin capsule. Following oral administration to diabetic rats, the intestinal fluid that has passed through the gelatin capsule saturates the mixture; concomitantly, DTPA dianhydride produces an acidic environment, while SBC decomposes to form CO2 bubbles at acidic pH. The gas bubbles grow among the surfactant molecules (SDS) owing to the expansion of the generated CO2. The walls of the CO2 bubbles consist of a self-assembled film of water that is in nanoscale and may serve as a colloidal carrier to transport insulin and DTPA. The grown gas bubbles continue to expand until they bump into the wall and burst, releasing their transported insulin, DTPA, and SDS into the mucosal layer. The released DTPA and SDS function as protease inhibitors to protect the insulin molecules as well as absorption enhancers to augment their epithelial permeability and eventual absorption into systemic circulation, exerting their hypoglycemic effects.

Original languageEnglish
Pages (from-to)115-124
Number of pages10
JournalBiomaterials
Volume64
DOIs
Publication statusPublished - Sep 1 2015

Fingerprint

Insulin
Carrier Proteins
Proteins
Acids
Gelatin
Surface-Active Agents
Capsules
Surface active agents
Gases
Sodium bicarbonate
Blowing agents
Sodium Bicarbonate
Molecules
Sodium dodecyl sulfate
Protease Inhibitors
Hypoglycemic Agents
Sodium Dodecyl Sulfate
Oral Administration
Rats
Permeability

Keywords

  • Biodistribution
  • Colloidal carrier
  • Foaming agent
  • Oral protein delivery
  • Surfactant

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Self-assembling bubble carriers for oral protein delivery. / Chuang, Er-Yuan; Lin, Kun Ju; Lin, Po Yen; Chen, Hsin Lung; Wey, Shiaw Pyng; Mi, Fwu Long; Hsiao, Hsu Chan; Chen, Chiung Tong; Sung, Hsing Wen.

In: Biomaterials, Vol. 64, 01.09.2015, p. 115-124.

Research output: Contribution to journalArticle

Chuang, E-Y, Lin, KJ, Lin, PY, Chen, HL, Wey, SP, Mi, FL, Hsiao, HC, Chen, CT & Sung, HW 2015, 'Self-assembling bubble carriers for oral protein delivery', Biomaterials, vol. 64, pp. 115-124. https://doi.org/10.1016/j.biomaterials.2015.06.035
Chuang, Er-Yuan ; Lin, Kun Ju ; Lin, Po Yen ; Chen, Hsin Lung ; Wey, Shiaw Pyng ; Mi, Fwu Long ; Hsiao, Hsu Chan ; Chen, Chiung Tong ; Sung, Hsing Wen. / Self-assembling bubble carriers for oral protein delivery. In: Biomaterials. 2015 ; Vol. 64. pp. 115-124.
@article{d4bde87813464edc99f15c78d5a6e823,
title = "Self-assembling bubble carriers for oral protein delivery",
abstract = "Successful oral delivery of therapeutic proteins such as insulin can greatly improve the quality of life of patients. This study develops a bubble carrier system by loading diethylene triamine pentaacetic acid (DTPA) dianhydride, a foaming agent (sodium bicarbonate; SBC), a surfactant (sodium dodecyl sulfate; SDS), and a protein drug (insulin) in an enteric-coated gelatin capsule. Following oral administration to diabetic rats, the intestinal fluid that has passed through the gelatin capsule saturates the mixture; concomitantly, DTPA dianhydride produces an acidic environment, while SBC decomposes to form CO2 bubbles at acidic pH. The gas bubbles grow among the surfactant molecules (SDS) owing to the expansion of the generated CO2. The walls of the CO2 bubbles consist of a self-assembled film of water that is in nanoscale and may serve as a colloidal carrier to transport insulin and DTPA. The grown gas bubbles continue to expand until they bump into the wall and burst, releasing their transported insulin, DTPA, and SDS into the mucosal layer. The released DTPA and SDS function as protease inhibitors to protect the insulin molecules as well as absorption enhancers to augment their epithelial permeability and eventual absorption into systemic circulation, exerting their hypoglycemic effects.",
keywords = "Biodistribution, Colloidal carrier, Foaming agent, Oral protein delivery, Surfactant",
author = "Er-Yuan Chuang and Lin, {Kun Ju} and Lin, {Po Yen} and Chen, {Hsin Lung} and Wey, {Shiaw Pyng} and Mi, {Fwu Long} and Hsiao, {Hsu Chan} and Chen, {Chiung Tong} and Sung, {Hsing Wen}",
year = "2015",
month = "9",
day = "1",
doi = "10.1016/j.biomaterials.2015.06.035",
language = "English",
volume = "64",
pages = "115--124",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier Science Ltd",

}

TY - JOUR

T1 - Self-assembling bubble carriers for oral protein delivery

AU - Chuang, Er-Yuan

AU - Lin, Kun Ju

AU - Lin, Po Yen

AU - Chen, Hsin Lung

AU - Wey, Shiaw Pyng

AU - Mi, Fwu Long

AU - Hsiao, Hsu Chan

AU - Chen, Chiung Tong

AU - Sung, Hsing Wen

PY - 2015/9/1

Y1 - 2015/9/1

N2 - Successful oral delivery of therapeutic proteins such as insulin can greatly improve the quality of life of patients. This study develops a bubble carrier system by loading diethylene triamine pentaacetic acid (DTPA) dianhydride, a foaming agent (sodium bicarbonate; SBC), a surfactant (sodium dodecyl sulfate; SDS), and a protein drug (insulin) in an enteric-coated gelatin capsule. Following oral administration to diabetic rats, the intestinal fluid that has passed through the gelatin capsule saturates the mixture; concomitantly, DTPA dianhydride produces an acidic environment, while SBC decomposes to form CO2 bubbles at acidic pH. The gas bubbles grow among the surfactant molecules (SDS) owing to the expansion of the generated CO2. The walls of the CO2 bubbles consist of a self-assembled film of water that is in nanoscale and may serve as a colloidal carrier to transport insulin and DTPA. The grown gas bubbles continue to expand until they bump into the wall and burst, releasing their transported insulin, DTPA, and SDS into the mucosal layer. The released DTPA and SDS function as protease inhibitors to protect the insulin molecules as well as absorption enhancers to augment their epithelial permeability and eventual absorption into systemic circulation, exerting their hypoglycemic effects.

AB - Successful oral delivery of therapeutic proteins such as insulin can greatly improve the quality of life of patients. This study develops a bubble carrier system by loading diethylene triamine pentaacetic acid (DTPA) dianhydride, a foaming agent (sodium bicarbonate; SBC), a surfactant (sodium dodecyl sulfate; SDS), and a protein drug (insulin) in an enteric-coated gelatin capsule. Following oral administration to diabetic rats, the intestinal fluid that has passed through the gelatin capsule saturates the mixture; concomitantly, DTPA dianhydride produces an acidic environment, while SBC decomposes to form CO2 bubbles at acidic pH. The gas bubbles grow among the surfactant molecules (SDS) owing to the expansion of the generated CO2. The walls of the CO2 bubbles consist of a self-assembled film of water that is in nanoscale and may serve as a colloidal carrier to transport insulin and DTPA. The grown gas bubbles continue to expand until they bump into the wall and burst, releasing their transported insulin, DTPA, and SDS into the mucosal layer. The released DTPA and SDS function as protease inhibitors to protect the insulin molecules as well as absorption enhancers to augment their epithelial permeability and eventual absorption into systemic circulation, exerting their hypoglycemic effects.

KW - Biodistribution

KW - Colloidal carrier

KW - Foaming agent

KW - Oral protein delivery

KW - Surfactant

UR - http://www.scopus.com/inward/record.url?scp=84937434579&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84937434579&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2015.06.035

DO - 10.1016/j.biomaterials.2015.06.035

M3 - Article

C2 - 26132436

AN - SCOPUS:84937434579

VL - 64

SP - 115

EP - 124

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

ER -