Self-assembled binary colloidal crystal monolayers as cell culture substrates

Peng Yuan Wang, Hitesh Pingle, Peter Koegler, Helmut Thissen, Peter Kingshott

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

This study investigated the formation of self-assembled binary colloidal crystal (BCC) monolayers using evaporation induced confined area assembly (EICAA), and fabricated a family of various BCCs for cell culture. A library of various BCCs with different structures was established and it was demonstrated that after stabilisation the BCCs have potential to be used as substrates with well-ordered surface topographies and chemistries for manipulating cell-surface interactions. Three cell types including MG63 osteoblasts, L929 fibroblasts, and primary human adipose-derived stem cells (hADSCs) show different responses on selected surfaces (either between BCCs or BCCs vs. flat controls). In general, cell spreading was inhibited on BCCs due to surface topography. However, the chemical composition presented on the BCCs can compensate the topographic effect depending on what combination was used. The ordered topographic and heterogeneous chemical patterns provide a complexity of surface properties and have potential to be selectively modified with desired biomolecules for controlling biointerface interactions.

Original languageEnglish
Pages (from-to)2545-2552
Number of pages8
JournalJournal of Materials Chemistry B
Volume3
Issue number12
DOIs
Publication statusPublished - Mar 28 2015
Externally publishedYes

Fingerprint

Surface topography
Cell culture
Monolayers
Cell Culture Techniques
Crystals
Surface Properties
Osteoblasts
Biomolecules
Substrates
Fibroblasts
Surface chemistry
Stem cells
Cell Communication
Libraries
Surface properties
Evaporation
Stem Cells
Stabilization
Chemical analysis

ASJC Scopus subject areas

  • Biomedical Engineering
  • Medicine(all)
  • Chemistry(all)
  • Materials Science(all)

Cite this

Wang, P. Y., Pingle, H., Koegler, P., Thissen, H., & Kingshott, P. (2015). Self-assembled binary colloidal crystal monolayers as cell culture substrates. Journal of Materials Chemistry B, 3(12), 2545-2552. https://doi.org/10.1039/c4tb02006e

Self-assembled binary colloidal crystal monolayers as cell culture substrates. / Wang, Peng Yuan; Pingle, Hitesh; Koegler, Peter; Thissen, Helmut; Kingshott, Peter.

In: Journal of Materials Chemistry B, Vol. 3, No. 12, 28.03.2015, p. 2545-2552.

Research output: Contribution to journalArticle

Wang, PY, Pingle, H, Koegler, P, Thissen, H & Kingshott, P 2015, 'Self-assembled binary colloidal crystal monolayers as cell culture substrates', Journal of Materials Chemistry B, vol. 3, no. 12, pp. 2545-2552. https://doi.org/10.1039/c4tb02006e
Wang, Peng Yuan ; Pingle, Hitesh ; Koegler, Peter ; Thissen, Helmut ; Kingshott, Peter. / Self-assembled binary colloidal crystal monolayers as cell culture substrates. In: Journal of Materials Chemistry B. 2015 ; Vol. 3, No. 12. pp. 2545-2552.
@article{6a1c89ebde034226a2bd927849e52104,
title = "Self-assembled binary colloidal crystal monolayers as cell culture substrates",
abstract = "This study investigated the formation of self-assembled binary colloidal crystal (BCC) monolayers using evaporation induced confined area assembly (EICAA), and fabricated a family of various BCCs for cell culture. A library of various BCCs with different structures was established and it was demonstrated that after stabilisation the BCCs have potential to be used as substrates with well-ordered surface topographies and chemistries for manipulating cell-surface interactions. Three cell types including MG63 osteoblasts, L929 fibroblasts, and primary human adipose-derived stem cells (hADSCs) show different responses on selected surfaces (either between BCCs or BCCs vs. flat controls). In general, cell spreading was inhibited on BCCs due to surface topography. However, the chemical composition presented on the BCCs can compensate the topographic effect depending on what combination was used. The ordered topographic and heterogeneous chemical patterns provide a complexity of surface properties and have potential to be selectively modified with desired biomolecules for controlling biointerface interactions.",
author = "Wang, {Peng Yuan} and Hitesh Pingle and Peter Koegler and Helmut Thissen and Peter Kingshott",
year = "2015",
month = "3",
day = "28",
doi = "10.1039/c4tb02006e",
language = "English",
volume = "3",
pages = "2545--2552",
journal = "Journal of Materials Chemistry B",
issn = "2050-7518",
publisher = "Royal Society of Chemistry",
number = "12",

}

TY - JOUR

T1 - Self-assembled binary colloidal crystal monolayers as cell culture substrates

AU - Wang, Peng Yuan

AU - Pingle, Hitesh

AU - Koegler, Peter

AU - Thissen, Helmut

AU - Kingshott, Peter

PY - 2015/3/28

Y1 - 2015/3/28

N2 - This study investigated the formation of self-assembled binary colloidal crystal (BCC) monolayers using evaporation induced confined area assembly (EICAA), and fabricated a family of various BCCs for cell culture. A library of various BCCs with different structures was established and it was demonstrated that after stabilisation the BCCs have potential to be used as substrates with well-ordered surface topographies and chemistries for manipulating cell-surface interactions. Three cell types including MG63 osteoblasts, L929 fibroblasts, and primary human adipose-derived stem cells (hADSCs) show different responses on selected surfaces (either between BCCs or BCCs vs. flat controls). In general, cell spreading was inhibited on BCCs due to surface topography. However, the chemical composition presented on the BCCs can compensate the topographic effect depending on what combination was used. The ordered topographic and heterogeneous chemical patterns provide a complexity of surface properties and have potential to be selectively modified with desired biomolecules for controlling biointerface interactions.

AB - This study investigated the formation of self-assembled binary colloidal crystal (BCC) monolayers using evaporation induced confined area assembly (EICAA), and fabricated a family of various BCCs for cell culture. A library of various BCCs with different structures was established and it was demonstrated that after stabilisation the BCCs have potential to be used as substrates with well-ordered surface topographies and chemistries for manipulating cell-surface interactions. Three cell types including MG63 osteoblasts, L929 fibroblasts, and primary human adipose-derived stem cells (hADSCs) show different responses on selected surfaces (either between BCCs or BCCs vs. flat controls). In general, cell spreading was inhibited on BCCs due to surface topography. However, the chemical composition presented on the BCCs can compensate the topographic effect depending on what combination was used. The ordered topographic and heterogeneous chemical patterns provide a complexity of surface properties and have potential to be selectively modified with desired biomolecules for controlling biointerface interactions.

UR - http://www.scopus.com/inward/record.url?scp=84924871547&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84924871547&partnerID=8YFLogxK

U2 - 10.1039/c4tb02006e

DO - 10.1039/c4tb02006e

M3 - Article

AN - SCOPUS:84924871547

VL - 3

SP - 2545

EP - 2552

JO - Journal of Materials Chemistry B

JF - Journal of Materials Chemistry B

SN - 2050-7518

IS - 12

ER -