Securin depletion sensitizes human colon cancer cells to fisetin-induced apoptosis

Sz Hsien Yu, Pei Ming Yang, Chih Wen Peng, Yi Chu Yu, Shu Jun Chiu

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Securin is highly-expressed in various tumors including those of the colon. In this study, the role of securin in the anticancer effects of fisetin on human colon cancer cells was investigated. Fisetin-induced apoptosis in HCT116 cells as indicated by TUNEL assay, Annexin V-FITC/PI double staining, Ser15-phosphorylation of p53, and cleavages of procaspase-3 and PARP. These effects were enhanced in HCT116 securin-null cells or in wild-type cells in which securin was knockdown by siRNA, but attenuated when wild-type or non-degradable securin was reconstituted. Moreover, fisetin did not induce apoptosis in HCT116 p53-null and HT-29 p53-mutant cells. Knockdown of securin in HCT116 p53-null cells potentiated fisetin-induced cytotoxicity by induction of apoptosis. Our results provide the first evidence to support that securin depletion sensitizes human colon cancer cells to fisetin-induced apoptosis.

Original languageEnglish
Pages (from-to)96-104
Number of pages9
JournalCancer Letters
Volume300
Issue number1
DOIs
Publication statusPublished - Jan 1 2011
Externally publishedYes

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Keywords

  • Fisetin
  • Human colon cancer cells
  • P53
  • Securin

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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