Securin depletion sensitizes human colon cancer cells to fisetin-induced apoptosis

Sz Hsien Yu, Pei Ming Yang, Chih Wen Peng, Yi Chu Yu, Shu Jun Chiu

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Securin is highly-expressed in various tumors including those of the colon. In this study, the role of securin in the anticancer effects of fisetin on human colon cancer cells was investigated. Fisetin-induced apoptosis in HCT116 cells as indicated by TUNEL assay, Annexin V-FITC/PI double staining, Ser15-phosphorylation of p53, and cleavages of procaspase-3 and PARP. These effects were enhanced in HCT116 securin-null cells or in wild-type cells in which securin was knockdown by siRNA, but attenuated when wild-type or non-degradable securin was reconstituted. Moreover, fisetin did not induce apoptosis in HCT116 p53-null and HT-29 p53-mutant cells. Knockdown of securin in HCT116 p53-null cells potentiated fisetin-induced cytotoxicity by induction of apoptosis. Our results provide the first evidence to support that securin depletion sensitizes human colon cancer cells to fisetin-induced apoptosis.

Original languageEnglish
Pages (from-to)96-104
Number of pages9
JournalCancer Letters
Volume300
Issue number1
DOIs
Publication statusPublished - Jan 1 2011
Externally publishedYes

Fingerprint

Securin
Colonic Neoplasms
Apoptosis
Null Lymphocytes
HCT116 Cells
Fluorescein-5-isothiocyanate
Annexin A5
In Situ Nick-End Labeling
fisetin
Caspase 3
Small Interfering RNA
Colon
Phosphorylation
Staining and Labeling

Keywords

  • Fisetin
  • Human colon cancer cells
  • P53
  • Securin

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Securin depletion sensitizes human colon cancer cells to fisetin-induced apoptosis. / Yu, Sz Hsien; Yang, Pei Ming; Peng, Chih Wen; Yu, Yi Chu; Chiu, Shu Jun.

In: Cancer Letters, Vol. 300, No. 1, 01.01.2011, p. 96-104.

Research output: Contribution to journalArticle

Yu, Sz Hsien ; Yang, Pei Ming ; Peng, Chih Wen ; Yu, Yi Chu ; Chiu, Shu Jun. / Securin depletion sensitizes human colon cancer cells to fisetin-induced apoptosis. In: Cancer Letters. 2011 ; Vol. 300, No. 1. pp. 96-104.
@article{e3bce577bd074a729d1a65cc9faba3f7,
title = "Securin depletion sensitizes human colon cancer cells to fisetin-induced apoptosis",
abstract = "Securin is highly-expressed in various tumors including those of the colon. In this study, the role of securin in the anticancer effects of fisetin on human colon cancer cells was investigated. Fisetin-induced apoptosis in HCT116 cells as indicated by TUNEL assay, Annexin V-FITC/PI double staining, Ser15-phosphorylation of p53, and cleavages of procaspase-3 and PARP. These effects were enhanced in HCT116 securin-null cells or in wild-type cells in which securin was knockdown by siRNA, but attenuated when wild-type or non-degradable securin was reconstituted. Moreover, fisetin did not induce apoptosis in HCT116 p53-null and HT-29 p53-mutant cells. Knockdown of securin in HCT116 p53-null cells potentiated fisetin-induced cytotoxicity by induction of apoptosis. Our results provide the first evidence to support that securin depletion sensitizes human colon cancer cells to fisetin-induced apoptosis.",
keywords = "Fisetin, Human colon cancer cells, P53, Securin",
author = "Yu, {Sz Hsien} and Yang, {Pei Ming} and Peng, {Chih Wen} and Yu, {Yi Chu} and Chiu, {Shu Jun}",
year = "2011",
month = "1",
day = "1",
doi = "10.1016/j.canlet.2010.09.015",
language = "English",
volume = "300",
pages = "96--104",
journal = "Cancer Letters",
issn = "0304-3835",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

TY - JOUR

T1 - Securin depletion sensitizes human colon cancer cells to fisetin-induced apoptosis

AU - Yu, Sz Hsien

AU - Yang, Pei Ming

AU - Peng, Chih Wen

AU - Yu, Yi Chu

AU - Chiu, Shu Jun

PY - 2011/1/1

Y1 - 2011/1/1

N2 - Securin is highly-expressed in various tumors including those of the colon. In this study, the role of securin in the anticancer effects of fisetin on human colon cancer cells was investigated. Fisetin-induced apoptosis in HCT116 cells as indicated by TUNEL assay, Annexin V-FITC/PI double staining, Ser15-phosphorylation of p53, and cleavages of procaspase-3 and PARP. These effects were enhanced in HCT116 securin-null cells or in wild-type cells in which securin was knockdown by siRNA, but attenuated when wild-type or non-degradable securin was reconstituted. Moreover, fisetin did not induce apoptosis in HCT116 p53-null and HT-29 p53-mutant cells. Knockdown of securin in HCT116 p53-null cells potentiated fisetin-induced cytotoxicity by induction of apoptosis. Our results provide the first evidence to support that securin depletion sensitizes human colon cancer cells to fisetin-induced apoptosis.

AB - Securin is highly-expressed in various tumors including those of the colon. In this study, the role of securin in the anticancer effects of fisetin on human colon cancer cells was investigated. Fisetin-induced apoptosis in HCT116 cells as indicated by TUNEL assay, Annexin V-FITC/PI double staining, Ser15-phosphorylation of p53, and cleavages of procaspase-3 and PARP. These effects were enhanced in HCT116 securin-null cells or in wild-type cells in which securin was knockdown by siRNA, but attenuated when wild-type or non-degradable securin was reconstituted. Moreover, fisetin did not induce apoptosis in HCT116 p53-null and HT-29 p53-mutant cells. Knockdown of securin in HCT116 p53-null cells potentiated fisetin-induced cytotoxicity by induction of apoptosis. Our results provide the first evidence to support that securin depletion sensitizes human colon cancer cells to fisetin-induced apoptosis.

KW - Fisetin

KW - Human colon cancer cells

KW - P53

KW - Securin

UR - http://www.scopus.com/inward/record.url?scp=78549273307&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78549273307&partnerID=8YFLogxK

U2 - 10.1016/j.canlet.2010.09.015

DO - 10.1016/j.canlet.2010.09.015

M3 - Article

C2 - 20974518

AN - SCOPUS:78549273307

VL - 300

SP - 96

EP - 104

JO - Cancer Letters

JF - Cancer Letters

SN - 0304-3835

IS - 1

ER -