Second messengers mediating the proliferation and collagen synthesis of tenocytes induced by low-level laser irradiation

Mei Hsiu Chen, Yun Chien Huang, Jui Sheng Sun, Yuan Hung Chao, Ming Hong Chen

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

For decades, low-level laser therapy (LLLT) has widespread applications in tendon-related injuries. Although the therapeutic effect of LLLT could be explained by photostimulation of target tissue and cells, how tenocytes sense photonic energy and convert them into cascades of cellular and molecular events is still not well understood. This study was designed to elucidate the effects of LLLT on cell proliferation and collagen synthesis by examining the associated second messengers including ATP, Ca2+, and nitric oxide using rat Achilles tenocytes. Moreover, proliferating cell nuclear antigen (PCNA) and transforming growth factor-β1 (TGF-β1) related to cell proliferation and matrix metabolism were also studied. The results showed that 904 nm GaAs laser of 1 J/cm2 could significantly increase the MTT activity and collagen synthesis of tenocytes. Second messengers including ATP and intracellular Ca2+ were increased after laser treatment. Quantitative PCR analysis of tenocytes treated with laser revealed up-regulated expression of PCNA, type I collagen, and TGF-β1. Besides, laser-induced TGF-β1 expression was significantly inhibited by extracellular signal-regulated kinase (ERK) specific inhibitor (PD98059). The findings suggested that LLLT stimulated ATP production and increased intracellular calcium concentration. Directly or indirectly via production of TGF-β1, these second messengers mediated the proliferation of tenocytes and synthesis of collagen.

Original languageEnglish
Pages (from-to)263-272
Number of pages10
JournalLasers in Medical Science
Volume30
Issue number1
DOIs
Publication statusPublished - Jan 1 2014
Externally publishedYes

Fingerprint

Second Messenger Systems
Transforming Growth Factors
Lasers
Collagen
Adenosine Triphosphate
Proliferating Cell Nuclear Antigen
Cell Proliferation
Optics and Photonics
Tendon Injuries
Semiconductor Lasers
Extracellular Signal-Regulated MAP Kinases
Therapeutic Uses
Collagen Type I
Nitric Oxide
Tenocytes
Calcium
Polymerase Chain Reaction
Low-Level Light Therapy

Keywords

  • ATP
  • Calcium
  • Collagen
  • ERK
  • Laser
  • Second messenger
  • Tendon
  • TGF-β1

ASJC Scopus subject areas

  • Surgery
  • Dermatology

Cite this

Second messengers mediating the proliferation and collagen synthesis of tenocytes induced by low-level laser irradiation. / Chen, Mei Hsiu; Huang, Yun Chien; Sun, Jui Sheng; Chao, Yuan Hung; Chen, Ming Hong.

In: Lasers in Medical Science, Vol. 30, No. 1, 01.01.2014, p. 263-272.

Research output: Contribution to journalArticle

Chen, Mei Hsiu ; Huang, Yun Chien ; Sun, Jui Sheng ; Chao, Yuan Hung ; Chen, Ming Hong. / Second messengers mediating the proliferation and collagen synthesis of tenocytes induced by low-level laser irradiation. In: Lasers in Medical Science. 2014 ; Vol. 30, No. 1. pp. 263-272.
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