Seasonal distribution and genotyping of antibiotic resistant strains of Listeria innocua isolated from a river basin categorized by ERIC-PCR

Hsin Chi Tsai, Ming Yuan Chou, Cheng Chun Wu, Min Tao Wan, Yi Jie Kuo, Jung Sheng Chen, Tung Yi Huang, Bing Mu Hsu

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Listeria innocua retains many conserved homologous domains with Listeria monocytogenes, which is a food-borne and water-borne diarrhea-causing bacterium. Studies of antimicrobial resistance in L. innocua showed that this microbe is more prone to acquire resistance than other bacteria in the genus Listeria. However, little is known about the seasonal population distribution and antimicrobial resistance patterns of L. innocua in natural water environments. The aims of the study were: (1) to investigate the occurrence of L. innocua isolates in a subtropical watershed and reconstruct the population structure and (2) to analyze the antibacterial resistance patterns of the identified L. innocua isolates according to ERIC type. A total of 288 water samples was collected from the Puzi River basin (23° 28’ N, 120° 13’ E) between March 2014 and March 2015, and 36 L. innocua isolates were recovered from 15 positive water samples. With regard to seasonal variation, L. innocua was only detected in the spring and summer. Eighteen enterobacterial repetitive intergenic consensus (ERIC)-PCR types were identified, and two genogroups with four subgroups were reconstructed in a minimum spanning tree. Isolates from different sampling areas that were located near each other were genetically different. All L. innocua isolates (including 41.7% of the multidrug-resistant (MDR) isolates) were resistant to oxacillin and showed high minimum inhibitory concentrations of tetracycline. These findings demonstrate the seasonal variations and differing geographical distributions of L. innocua in this subtropical water environment, as well as the existence of strong population structures and MDR and antimicrobial resistance patterns. Phylogenetic analysis based on ERIC-type showed that the Cluster A isolates were resistant to more antibiotics, and two types, ERIC8 and ERIC15 were multidrug resistant. The more commonly detected types, such as ERIC1 and ERIC12, were also more likely to be resistant to two or more antibiotics. Close monitoring of drug resistance in environmental L. innocua is warranted due to its potential for transferring antimicrobial resistance determinants to pathogenic Listeria.

Original languageEnglish
Article number1559
JournalInternational Journal of Environmental Research and Public Health
Volume15
Issue number7
DOIs
Publication statusPublished - Jul 23 2018

Fingerprint

Listeria
Rivers
Anti-Bacterial Agents
Polymerase Chain Reaction
Water
Bacteria
Oxacillin
Listeria monocytogenes
Microbial Sensitivity Tests
Tetracycline
Drug Resistance
Population
Diarrhea
Genotype
Demography
Food

Keywords

  • Antimicrobial
  • Diversity
  • ERIC-PCR
  • Listeria innocua
  • Resistance
  • River basin

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

Cite this

Seasonal distribution and genotyping of antibiotic resistant strains of Listeria innocua isolated from a river basin categorized by ERIC-PCR. / Tsai, Hsin Chi; Chou, Ming Yuan; Wu, Cheng Chun; Wan, Min Tao; Kuo, Yi Jie; Chen, Jung Sheng; Huang, Tung Yi; Hsu, Bing Mu.

In: International Journal of Environmental Research and Public Health, Vol. 15, No. 7, 1559, 23.07.2018.

Research output: Contribution to journalArticle

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AU - Kuo, Yi Jie

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AB - Listeria innocua retains many conserved homologous domains with Listeria monocytogenes, which is a food-borne and water-borne diarrhea-causing bacterium. Studies of antimicrobial resistance in L. innocua showed that this microbe is more prone to acquire resistance than other bacteria in the genus Listeria. However, little is known about the seasonal population distribution and antimicrobial resistance patterns of L. innocua in natural water environments. The aims of the study were: (1) to investigate the occurrence of L. innocua isolates in a subtropical watershed and reconstruct the population structure and (2) to analyze the antibacterial resistance patterns of the identified L. innocua isolates according to ERIC type. A total of 288 water samples was collected from the Puzi River basin (23° 28’ N, 120° 13’ E) between March 2014 and March 2015, and 36 L. innocua isolates were recovered from 15 positive water samples. With regard to seasonal variation, L. innocua was only detected in the spring and summer. Eighteen enterobacterial repetitive intergenic consensus (ERIC)-PCR types were identified, and two genogroups with four subgroups were reconstructed in a minimum spanning tree. Isolates from different sampling areas that were located near each other were genetically different. All L. innocua isolates (including 41.7% of the multidrug-resistant (MDR) isolates) were resistant to oxacillin and showed high minimum inhibitory concentrations of tetracycline. These findings demonstrate the seasonal variations and differing geographical distributions of L. innocua in this subtropical water environment, as well as the existence of strong population structures and MDR and antimicrobial resistance patterns. Phylogenetic analysis based on ERIC-type showed that the Cluster A isolates were resistant to more antibiotics, and two types, ERIC8 and ERIC15 were multidrug resistant. The more commonly detected types, such as ERIC1 and ERIC12, were also more likely to be resistant to two or more antibiotics. Close monitoring of drug resistance in environmental L. innocua is warranted due to its potential for transferring antimicrobial resistance determinants to pathogenic Listeria.

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