Screening of aptamers specific to colorectal cancer cells and stem cells by utilizing On-chip Cell-SELEX

Lien Yu Hung, Chih Hung Wang, Yu Jui Che, Chien Yu Fu, Hwan You Chang, Kuan Wang, Gwo Bin Lee

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Colorectal cancer (CRC) is the most frequently diagnosed cancer around the world, causing about 700,000 deaths every year. It is clear now that a small fraction of CRC, named colorectal cancer stem cells (CSCs) exhibiting self-renewal and extensive proliferative activities, are hard to be eradicated. Unfortunately, highly specific biomarkers for colorectal CSC (CR-CSCs) are lacking that prohibits the development of effective therapeutic strategies. This study designed and manufactured a novel microfluidic system capable of performing a fully automated cell-based, systematic evolution of ligands by exponential enrichment (SELEX) process. Eight CR-CSC/CRC-specific aptamers were successfully selected using the microfluidic chip. Three of the aptamers showed high affinities towards their respective target cells with a dissociation constant of 27.4, 28.5 and 12.3 nM, which are comparable to that of antibodies.

Original languageEnglish
Article number10326
JournalScientific Reports
Volume5
DOIs
Publication statusPublished - May 22 2015
Externally publishedYes

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SELEX Aptamer Technique
Immunomagnetic Separation
Nucleotide Aptamers
Microfluidic Analytical Techniques
Neoplastic Stem Cells
Single-Stranded DNA
Tumor Biomarkers
Tumor Cell Line
Colorectal Neoplasms
Flow Cytometry
Stem Cells
Microfluidics
Biomarkers
Antibodies
Neoplasms

ASJC Scopus subject areas

  • General

Cite this

Screening of aptamers specific to colorectal cancer cells and stem cells by utilizing On-chip Cell-SELEX. / Hung, Lien Yu; Wang, Chih Hung; Che, Yu Jui; Fu, Chien Yu; Chang, Hwan You; Wang, Kuan; Lee, Gwo Bin.

In: Scientific Reports, Vol. 5, 10326, 22.05.2015.

Research output: Contribution to journalArticle

Hung, Lien Yu ; Wang, Chih Hung ; Che, Yu Jui ; Fu, Chien Yu ; Chang, Hwan You ; Wang, Kuan ; Lee, Gwo Bin. / Screening of aptamers specific to colorectal cancer cells and stem cells by utilizing On-chip Cell-SELEX. In: Scientific Reports. 2015 ; Vol. 5.
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