Screening mesenchymal stem cell attachment and differentiation on porous silicon gradients

Peng Yuan Wang, Lauren R. Clements, Helmut Thissen, Andrew Jane, Wei Bor Tsai, Nicolas H. Voelcker

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

The profound effects that nanoscale surface topography exerts on cell behavior are highly relevant to the development of advanced biomaterials and to advances in tissue engineering and regenerative medicine. Here, an asymmetric anodization procedure is used to produce n-type porous silicon (pSi) gradients with pore sizes ranging from tens to hundreds of nanometers in diameter and changes in the ridge nanoroughness from a few to tens nanometers. Rat mesenchymal stem cells (rMSCs) adhere poorly at the regions with small pore size but high ridge roughness. Cell adhesion is increased gradually towards the large pore size but low ridge roughness end of the pSi gradients. Surface topography influences cell differentiation, but not cell proliferation. Osteogenesis of rMSCs is enhanced by porous topography with a ridge roughness lower than 10 nm, while adipogenesis of rMSCs is enhanced on the entire pSi gradient compared with flat Si substrates. The results demonstrate that the gradient format allows in-depth screening of surface parameters that are important for the control of mammalian cell behavior, thereby advancing the development of new and improved biomaterials for orthopaedic and tissue engineering applications. An asymmetric anodization procedure is used to produce n-type porous silicon (pSi) gradients with pore sizes ranging from tens to hundreds of nanometers in diameter and changes in the ridge nanoroughness from a few to tens of nanometers. The results demonstrate that the gradient format allows in-depth screening of surface parameters that are important for the control of mammalian cell behavior, thereby advancing the development of new and improved biomaterials for orthopaedic and tissue engineering applications.

Original languageEnglish
Pages (from-to)3414-3423
Number of pages10
JournalAdvanced Functional Materials
Volume22
Issue number16
DOIs
Publication statusPublished - Aug 21 2012
Externally publishedYes

Fingerprint

stem cells
Porous silicon
Stem cells
porous silicon
Pore size
attachment
Screening
Biocompatible Materials
screening
Tissue engineering
Biomaterials
ridges
Rats
gradients
tissue engineering
Surface roughness
Orthopedics
Surface topography
rats
porosity

Keywords

  • adipogenesis
  • gradients
  • mesenchymal stem cells
  • osteogenesis
  • porous silicon
  • topography

ASJC Scopus subject areas

  • Biomaterials
  • Electrochemistry
  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials

Cite this

Wang, P. Y., Clements, L. R., Thissen, H., Jane, A., Tsai, W. B., & Voelcker, N. H. (2012). Screening mesenchymal stem cell attachment and differentiation on porous silicon gradients. Advanced Functional Materials, 22(16), 3414-3423. https://doi.org/10.1002/adfm.201200447

Screening mesenchymal stem cell attachment and differentiation on porous silicon gradients. / Wang, Peng Yuan; Clements, Lauren R.; Thissen, Helmut; Jane, Andrew; Tsai, Wei Bor; Voelcker, Nicolas H.

In: Advanced Functional Materials, Vol. 22, No. 16, 21.08.2012, p. 3414-3423.

Research output: Contribution to journalArticle

Wang, PY, Clements, LR, Thissen, H, Jane, A, Tsai, WB & Voelcker, NH 2012, 'Screening mesenchymal stem cell attachment and differentiation on porous silicon gradients', Advanced Functional Materials, vol. 22, no. 16, pp. 3414-3423. https://doi.org/10.1002/adfm.201200447
Wang, Peng Yuan ; Clements, Lauren R. ; Thissen, Helmut ; Jane, Andrew ; Tsai, Wei Bor ; Voelcker, Nicolas H. / Screening mesenchymal stem cell attachment and differentiation on porous silicon gradients. In: Advanced Functional Materials. 2012 ; Vol. 22, No. 16. pp. 3414-3423.
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