Screening and confirmation of 62 drugs of abuse and metabolites in urine by ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry

I. Lin Tsai, Te I. Weng, Yufeng J. Tseng, Happy Kuy Lok Tan, Hsiao Ju Sun, Ching Hua Kuo

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

An ultra-high-performance liquid chromatography-quadrupole timeof-flight mass spectrometry (UHPLC-QTOF-MS) method for the screening and confirmation of 62 drugs of abuse and their metabolites in urine was developed in this study. The most commonly abused drugs, including amphetamines, opioids, cocaine, benzodiazepines (BZDs) and barbiturates, and many other new and emerging abused drugs, were selected as the analytes for this study. Urine samples were diluted 5-fold with deionized water before analysis. Using a superficially porous micro-particulate column and an acetic acid-based mobile phase, 54 basic and 8 acidic analytes could be detected within 15 and 12 min in positive and negative ionization modes, respectively. The MS collision energies for the 62 analytes were optimized, and their respective fragmentation patterns were constructed in the in-house library for confirmatory analysis. The coefficients of variation of the intra- and inter-day precision of the analyte responses all were

Original languageEnglish
Article numberbkt083
Pages (from-to)642-651
Number of pages10
JournalJournal of Analytical Toxicology
Volume37
Issue number9
DOIs
Publication statusPublished - Nov 2013
Externally publishedYes

Fingerprint

Water analysis
Deionized water
High performance liquid chromatography
Street Drugs
Metabolites
Acetic acid
urine
Ionization
Mass spectrometry
liquid chromatography
Mass Spectrometry
metabolite
Screening
drug
mass spectrometry
High Pressure Liquid Chromatography
Urine
Amphetamines
Barbiturates
Benzodiazepines

ASJC Scopus subject areas

  • Analytical Chemistry
  • Toxicology
  • Health, Toxicology and Mutagenesis
  • Chemical Health and Safety
  • Environmental Chemistry

Cite this

Screening and confirmation of 62 drugs of abuse and metabolites in urine by ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry. / Tsai, I. Lin; Weng, Te I.; Tseng, Yufeng J.; Tan, Happy Kuy Lok; Sun, Hsiao Ju; Kuo, Ching Hua.

In: Journal of Analytical Toxicology, Vol. 37, No. 9, bkt083, 11.2013, p. 642-651.

Research output: Contribution to journalArticle

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