Sclareol exhibits anti-inflammatory activity in both lipopolysaccharide- stimulated macrophages and the λ-carrageenan-induced paw edema model

Guan Jhong Huang, Chun Hsu Pan, Chieh His Wu

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Sclareol (1) is a natural fragrance compound used widely in the cosmetic and food industries. Lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and the λ-carrageenan-induced edema mouse paw model were applied to examine the anti-inflammatory potential of 1 and its possible molecular mechanisms. The experimental results obtained demonstrated that this compound inhibited cell growth, nitric oxide (NO) production, and the expression of the inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) proteins in LPS-stimulated macrophages. Compound 1 also reduced paw edema, the tissue content of NO, tumor necrosis factor-alpha (TNF-α), malondialdehyde (MDA), iNOS and COX-2 protein expression, and neutrophil infiltration within the tissues after λ-carrageenan stimulation. The present study suggests that the anti-inflammatory mechanisms of 1 might be related to a decrease of inflammatory cytokines and an increase of antioxidant enzyme activity.

Original languageEnglish
Pages (from-to)54-59
Number of pages6
JournalJournal of Natural Products
Volume75
Issue number1
DOIs
Publication statusPublished - Jan 27 2012
Externally publishedYes

Fingerprint

Macrophages
Carrageenan
Nitric Oxide Synthase Type II
Cyclooxygenase 2
Lipopolysaccharides
Edema
Nitric Oxide
Anti-Inflammatory Agents
Tissue
Fragrances
Cosmetics
Neutrophil Infiltration
Food Industry
Enzyme activity
Cell growth
Malondialdehyde
Infiltration
Proteins
Tumor Necrosis Factor-alpha
Antioxidants

ASJC Scopus subject areas

  • Complementary and alternative medicine
  • Molecular Medicine
  • Organic Chemistry
  • Analytical Chemistry
  • Pharmaceutical Science
  • Pharmacology
  • Drug Discovery

Cite this

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abstract = "Sclareol (1) is a natural fragrance compound used widely in the cosmetic and food industries. Lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and the λ-carrageenan-induced edema mouse paw model were applied to examine the anti-inflammatory potential of 1 and its possible molecular mechanisms. The experimental results obtained demonstrated that this compound inhibited cell growth, nitric oxide (NO) production, and the expression of the inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) proteins in LPS-stimulated macrophages. Compound 1 also reduced paw edema, the tissue content of NO, tumor necrosis factor-alpha (TNF-α), malondialdehyde (MDA), iNOS and COX-2 protein expression, and neutrophil infiltration within the tissues after λ-carrageenan stimulation. The present study suggests that the anti-inflammatory mechanisms of 1 might be related to a decrease of inflammatory cytokines and an increase of antioxidant enzyme activity.",
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