Schedule to inject in vitro matured oocytes may increase pregnancy after intracytoplasmic sperm injection

S. U. Chen, H. F. Chen, Y. R. Lien, H. N. Ho, H. C. Chang, Yu Shih Yang

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

To ascertain the value of using immature oocytes in an intracytoplasmic sperm injection (ICSI) program, the authors designed a schedule, at 5 p.m. on day 1 (the day of oocyte retrieval) and at 8 a.m. and 2 p.m. on day 2, to recognize and inject the in vitro matured (IVM) oocytes. For the 1166 oocytes retrieved in 107 ICSI cycles, 128 (11.0%) were at the stage of metaphase I (MI) and 113 (9.7%) at germinal vesicle. Routine ICSI for metaphase II oocytes was performed at 2 p.m. on day 1 (initial ICSI). In culture medium of human tubal fluid with 15% maternal serum, 85.1% (205/241) immature oocytes progressed to maturation in which 16.4% (21/128) of MI oocytes matured at 5 p.m. of day 1. The rate of normal fertilization for IVM oocytes (58.5%) was not significantly different from that of initial ICSI (64.0%). One patient received a transfer of two fertilized IVM oocytes alone that were injected at 5 p.m. of day 1, maturing from the MI stage, and achieved a normal pregnancy. The fertilized IVM oocytes were replaced along with the embryos from initial ICSI for 40 cycles that led to 14 (35%) clinical pregnancies. In 43 fertilized IVM oocytes donated for research, we observed that cleavage (95.3%) to the 2- to 4-cell stage was not distinct from that of initial ICSI (94.6%); however, the percentage of embryos of grade I and II morphology was significantly smaller (24.4% vs. 62.5%). Only five (11.6%) developed to blastocysts in vitro. Twenty-one fertilized IVM oocytes were frozen for future transfer. A schedule to inject IVM oocytes in ICSI cycles may generate more accessible embryos for fresh transfer or cryopreservation to increase the chance of pregnancy, although the embryo quality was relatively poor.

Original languageEnglish
Pages (from-to)197-205
Number of pages9
JournalArchives of Andrology
Volume44
Issue number3
Publication statusPublished - Jun 22 2000
Externally publishedYes

Fingerprint

Intracytoplasmic Sperm Injections
Ovulation Induction
Embryo Transfer
Pregnancy Rate
Oocytes
Appointments and Schedules
Pregnancy
Metaphase
Embryonic Structures
In Vitro Techniques
Oocyte Retrieval
Cryopreservation
Blastocyst
Fertilization in Vitro
Culture Media
Mothers

Keywords

  • In vitro matured oocytes
  • Intracytoplasmic sperm injection
  • Pregnancy

ASJC Scopus subject areas

  • Endocrinology

Cite this

Chen, S. U., Chen, H. F., Lien, Y. R., Ho, H. N., Chang, H. C., & Yang, Y. S. (2000). Schedule to inject in vitro matured oocytes may increase pregnancy after intracytoplasmic sperm injection. Archives of Andrology, 44(3), 197-205.

Schedule to inject in vitro matured oocytes may increase pregnancy after intracytoplasmic sperm injection. / Chen, S. U.; Chen, H. F.; Lien, Y. R.; Ho, H. N.; Chang, H. C.; Yang, Yu Shih.

In: Archives of Andrology, Vol. 44, No. 3, 22.06.2000, p. 197-205.

Research output: Contribution to journalArticle

Chen, SU, Chen, HF, Lien, YR, Ho, HN, Chang, HC & Yang, YS 2000, 'Schedule to inject in vitro matured oocytes may increase pregnancy after intracytoplasmic sperm injection', Archives of Andrology, vol. 44, no. 3, pp. 197-205.
Chen, S. U. ; Chen, H. F. ; Lien, Y. R. ; Ho, H. N. ; Chang, H. C. ; Yang, Yu Shih. / Schedule to inject in vitro matured oocytes may increase pregnancy after intracytoplasmic sperm injection. In: Archives of Andrology. 2000 ; Vol. 44, No. 3. pp. 197-205.
@article{a5e1cf8527bb40a384dd5c223d29c936,
title = "Schedule to inject in vitro matured oocytes may increase pregnancy after intracytoplasmic sperm injection",
abstract = "To ascertain the value of using immature oocytes in an intracytoplasmic sperm injection (ICSI) program, the authors designed a schedule, at 5 p.m. on day 1 (the day of oocyte retrieval) and at 8 a.m. and 2 p.m. on day 2, to recognize and inject the in vitro matured (IVM) oocytes. For the 1166 oocytes retrieved in 107 ICSI cycles, 128 (11.0{\%}) were at the stage of metaphase I (MI) and 113 (9.7{\%}) at germinal vesicle. Routine ICSI for metaphase II oocytes was performed at 2 p.m. on day 1 (initial ICSI). In culture medium of human tubal fluid with 15{\%} maternal serum, 85.1{\%} (205/241) immature oocytes progressed to maturation in which 16.4{\%} (21/128) of MI oocytes matured at 5 p.m. of day 1. The rate of normal fertilization for IVM oocytes (58.5{\%}) was not significantly different from that of initial ICSI (64.0{\%}). One patient received a transfer of two fertilized IVM oocytes alone that were injected at 5 p.m. of day 1, maturing from the MI stage, and achieved a normal pregnancy. The fertilized IVM oocytes were replaced along with the embryos from initial ICSI for 40 cycles that led to 14 (35{\%}) clinical pregnancies. In 43 fertilized IVM oocytes donated for research, we observed that cleavage (95.3{\%}) to the 2- to 4-cell stage was not distinct from that of initial ICSI (94.6{\%}); however, the percentage of embryos of grade I and II morphology was significantly smaller (24.4{\%} vs. 62.5{\%}). Only five (11.6{\%}) developed to blastocysts in vitro. Twenty-one fertilized IVM oocytes were frozen for future transfer. A schedule to inject IVM oocytes in ICSI cycles may generate more accessible embryos for fresh transfer or cryopreservation to increase the chance of pregnancy, although the embryo quality was relatively poor.",
keywords = "In vitro matured oocytes, Intracytoplasmic sperm injection, Pregnancy",
author = "Chen, {S. U.} and Chen, {H. F.} and Lien, {Y. R.} and Ho, {H. N.} and Chang, {H. C.} and Yang, {Yu Shih}",
year = "2000",
month = "6",
day = "22",
language = "English",
volume = "44",
pages = "197--205",
journal = "Systems Biology in Reproductive Medicine",
issn = "1939-6376",
publisher = "Informa Healthcare",
number = "3",

}

TY - JOUR

T1 - Schedule to inject in vitro matured oocytes may increase pregnancy after intracytoplasmic sperm injection

AU - Chen, S. U.

AU - Chen, H. F.

AU - Lien, Y. R.

AU - Ho, H. N.

AU - Chang, H. C.

AU - Yang, Yu Shih

PY - 2000/6/22

Y1 - 2000/6/22

N2 - To ascertain the value of using immature oocytes in an intracytoplasmic sperm injection (ICSI) program, the authors designed a schedule, at 5 p.m. on day 1 (the day of oocyte retrieval) and at 8 a.m. and 2 p.m. on day 2, to recognize and inject the in vitro matured (IVM) oocytes. For the 1166 oocytes retrieved in 107 ICSI cycles, 128 (11.0%) were at the stage of metaphase I (MI) and 113 (9.7%) at germinal vesicle. Routine ICSI for metaphase II oocytes was performed at 2 p.m. on day 1 (initial ICSI). In culture medium of human tubal fluid with 15% maternal serum, 85.1% (205/241) immature oocytes progressed to maturation in which 16.4% (21/128) of MI oocytes matured at 5 p.m. of day 1. The rate of normal fertilization for IVM oocytes (58.5%) was not significantly different from that of initial ICSI (64.0%). One patient received a transfer of two fertilized IVM oocytes alone that were injected at 5 p.m. of day 1, maturing from the MI stage, and achieved a normal pregnancy. The fertilized IVM oocytes were replaced along with the embryos from initial ICSI for 40 cycles that led to 14 (35%) clinical pregnancies. In 43 fertilized IVM oocytes donated for research, we observed that cleavage (95.3%) to the 2- to 4-cell stage was not distinct from that of initial ICSI (94.6%); however, the percentage of embryos of grade I and II morphology was significantly smaller (24.4% vs. 62.5%). Only five (11.6%) developed to blastocysts in vitro. Twenty-one fertilized IVM oocytes were frozen for future transfer. A schedule to inject IVM oocytes in ICSI cycles may generate more accessible embryos for fresh transfer or cryopreservation to increase the chance of pregnancy, although the embryo quality was relatively poor.

AB - To ascertain the value of using immature oocytes in an intracytoplasmic sperm injection (ICSI) program, the authors designed a schedule, at 5 p.m. on day 1 (the day of oocyte retrieval) and at 8 a.m. and 2 p.m. on day 2, to recognize and inject the in vitro matured (IVM) oocytes. For the 1166 oocytes retrieved in 107 ICSI cycles, 128 (11.0%) were at the stage of metaphase I (MI) and 113 (9.7%) at germinal vesicle. Routine ICSI for metaphase II oocytes was performed at 2 p.m. on day 1 (initial ICSI). In culture medium of human tubal fluid with 15% maternal serum, 85.1% (205/241) immature oocytes progressed to maturation in which 16.4% (21/128) of MI oocytes matured at 5 p.m. of day 1. The rate of normal fertilization for IVM oocytes (58.5%) was not significantly different from that of initial ICSI (64.0%). One patient received a transfer of two fertilized IVM oocytes alone that were injected at 5 p.m. of day 1, maturing from the MI stage, and achieved a normal pregnancy. The fertilized IVM oocytes were replaced along with the embryos from initial ICSI for 40 cycles that led to 14 (35%) clinical pregnancies. In 43 fertilized IVM oocytes donated for research, we observed that cleavage (95.3%) to the 2- to 4-cell stage was not distinct from that of initial ICSI (94.6%); however, the percentage of embryos of grade I and II morphology was significantly smaller (24.4% vs. 62.5%). Only five (11.6%) developed to blastocysts in vitro. Twenty-one fertilized IVM oocytes were frozen for future transfer. A schedule to inject IVM oocytes in ICSI cycles may generate more accessible embryos for fresh transfer or cryopreservation to increase the chance of pregnancy, although the embryo quality was relatively poor.

KW - In vitro matured oocytes

KW - Intracytoplasmic sperm injection

KW - Pregnancy

UR - http://www.scopus.com/inward/record.url?scp=79961131161&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79961131161&partnerID=8YFLogxK

M3 - Article

C2 - 10864367

AN - SCOPUS:79961131161

VL - 44

SP - 197

EP - 205

JO - Systems Biology in Reproductive Medicine

JF - Systems Biology in Reproductive Medicine

SN - 1939-6376

IS - 3

ER -