Scavenging effects of lotus seed extracts on reactive nitrogen species

Gow Chin Yen, Pin Der Duh, Hui Ju Su, Chi Tai Yeh, Chi Hao Wu

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

The inhibitory effect of lotus (Nelumbo nicifera) seed extracts extracted with water (LSWE), ethyl acetate (LSEAE) and hexane (LSHE) on reactive nitrogen species, induced by DNA damage in macrophage RAW 264.7 cells, was investigated. The results showed that all extracts could inhibit nitric oxide accumulation in LPS-activated RAW 264.7 cells. The extracts in the range of 0.01-0.2 mg/ml showed a dose-dependent inhibitory effect on the accumulation of nitric oxide upon decomposition of sodium nitroprusside (SNP). The potency of inhibitory activity was in the order: LSEAE > LSWE > LSHE. The results of the comet assay indicated that the three extracts could inhibit DNA damage in macrophage RAW 264.7 cells induced by SNP. In addition, the three samples, at 0.2 mg/ml, showed 63%, 59%, and 38% inhibition of DNA damage in macrophage RAW 264.7 cells induced by peroxynitrite, respectively. All extracts tested were found to be potent peroxynitrite scavengers, capable of preventing the nitration of tyrosine. The data obtained suggest that lotus seed extracts might act as chemopreventers through reduction of excess amounts of nitric oxide.

Original languageEnglish
Pages (from-to)596-602
Number of pages7
JournalFood Chemistry
Volume94
Issue number4
DOIs
Publication statusPublished - Mar 2006
Externally publishedYes

Fingerprint

Reactive Nitrogen Species
Macrophages
seed extracts
Scavenging
hydrophobicity
Seed
Seeds
Nitric Oxide
Peroxynitrous Acid
Hexanes
Nitroprusside
DNA damage
DNA Damage
nitric oxide
macrophages
DNA
extracts
nitrogen
hexane
Nitration

Keywords

  • Lotus seed
  • Macrophage RAW 264.7
  • Nitric oxide
  • Peroxynitrite
  • Single cell gel electrophoresis

ASJC Scopus subject areas

  • Food Science

Cite this

Scavenging effects of lotus seed extracts on reactive nitrogen species. / Yen, Gow Chin; Duh, Pin Der; Su, Hui Ju; Yeh, Chi Tai; Wu, Chi Hao.

In: Food Chemistry, Vol. 94, No. 4, 03.2006, p. 596-602.

Research output: Contribution to journalArticle

Yen, Gow Chin ; Duh, Pin Der ; Su, Hui Ju ; Yeh, Chi Tai ; Wu, Chi Hao. / Scavenging effects of lotus seed extracts on reactive nitrogen species. In: Food Chemistry. 2006 ; Vol. 94, No. 4. pp. 596-602.
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