Salvage of pyrimidine nucleosides by Trichomonas vaginalis

Ching Chung Wang, Hui Wen Cheng

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Trichomonas vaginalis is incapable of de novo pyrimidine biosynthesis because it cannot incorporate bicarbonate, aspartate or orotate into its pyrimidine nucleotides or nucleic acids. The organism can salvage exogenous cytidine > uridine > uracil and thymidine, and incorporate them into the nucleotide pool. A portion of cytidine is converted to CMP, CDP and CTP by cytidine phosphotransferase and nucleotide kinases. Some cytidine and most of uracil are, however, converted first to uridine by cytidine deaminase and uridine phosphorylase respectively; uridine is then incorporated into UMP, UDP and UTP by uridine phosphotransferase and nucleotide kinases. The two phosphotransferases, found mainly in the non-sedimentable fraction of T. vaginalis, provide the main avenue of pyrimidine salvage. No significant levels of pyrimidine phosphoribosyl transferase or nucleoside kinases can be detected in the extract. T. vaginalis has no appreciable dihydrofolate reductase or thymidylate synthetase; it grows normally in millimolar concentrations of methotrexate, pyrimethamine, or trimethoprim, and cannot incorporate labels from exogenous uracil or uridine into DNA. It has an enzyme thymidine phosphotransferase in the sedimentable fraction which converts thymidine to TMP. Thymidine salvage in T. vaginalis is thus totally isolated from the rest of the pyrimidine salvage.

Original languageEnglish
Pages (from-to)171-184
Number of pages14
JournalMolecular and Biochemical Parasitology
Volume10
Issue number2
DOIs
Publication statusPublished - 1984
Externally publishedYes

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Pyrimidine Nucleosides
Trichomonas vaginalis
Cytidine
Uridine
Phosphotransferases
Thymidine
Uracil
nucleoside phosphotransferase
dihydrofolate synthetase
Uridine Phosphorylase
Uracil Nucleotides
Pyrimidine Nucleotides
Cytidine Diphosphate
Thymidine Monophosphate
Cytidine Deaminase
Uridine Monophosphate
Cytidine Monophosphate
Cytidine Triphosphate
Pyrimethamine
Thymidylate Synthase

Keywords

  • Cytidine phosphotransferase
  • Pyrimidine nucleotide pool
  • Thymidine phosphotransferase
  • Trichomonas vaginalis
  • Uridine phosphotransferase

ASJC Scopus subject areas

  • Molecular Biology
  • Parasitology

Cite this

Salvage of pyrimidine nucleosides by Trichomonas vaginalis. / Wang, Ching Chung; Cheng, Hui Wen.

In: Molecular and Biochemical Parasitology, Vol. 10, No. 2, 1984, p. 171-184.

Research output: Contribution to journalArticle

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