Salutary effects of cepharanthine against skeletal muscle and kidney injuries following limb ischemia/reperfusion

Ming Chang Kao, Chih Yang Chung, Ya Ying Chang, Chih Kung Lin, Joen Rong Sheu, Chun Jen Huang

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Limb ischemia/reperfusion (I/R) causes oxidation and inflammation and subsequently induces muscle and kidney injuries. Cepharanthine, a natural plant alkaloid, possesses anti-inflammatory and antioxidative properties. We elucidated the salutary effects of cepharanthine against muscle and kidney injuries following limb I/R. Adult male rats were randomized to receive I/R or I/R plus cepharanthine. I/R was achieved by applying tourniquet high around each thigh for 3 hours followed by reperfusion for 24 hours. Cepharanthine (10 mg/kg, intraperitoneal) was injected immediately before reperfusion. After euthanization, degrees of tissue injury, inflammation, and oxidation were examined. Our data revealed that the I/R group had significant increases in injury biomarker concentrations of muscle (creatine kinase and lactate dehydrogenase) and kidney (creatinine, neutrophil gelatinase-associated lipocalin, and kidney injury molecule-1). Histological assays revealed moderate muscle and kidney injury characteristics in the I/R group. The I/R group also had significant increases in concentrations of inflammatory molecules (interleukin-6, macrophage inflammatory protein-2, and prostaglandin E and reactive nitrogen species (nitric oxide) as well as lipid peroxidation (malondialdehyde). Of note, these effects of limb I/R could be mitigated by cepharanthine. These data confirmed that cepharanthine attenuated muscle and kidney injuries induced by limb I/R. The mechanisms may involve its anti-inflammatory and antioxidative capacities.

Original languageEnglish
Article number504061
JournalEvidence-based Complementary and Alternative Medicine
Volume2015
DOIs
Publication statusPublished - 2015

Fingerprint

Reperfusion
Skeletal Muscle
Ischemia
Extremities
Kidney
Wounds and Injuries
Muscles
Anti-Inflammatory Agents
cepharanthine
MM Form Creatine Kinase
Chemokine CXCL2
Inflammation
Reactive Nitrogen Species
Tourniquets
Thigh
Prostaglandins E
Malondialdehyde
L-Lactate Dehydrogenase
Alkaloids
Lipid Peroxidation

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

Salutary effects of cepharanthine against skeletal muscle and kidney injuries following limb ischemia/reperfusion. / Kao, Ming Chang; Chung, Chih Yang; Chang, Ya Ying; Lin, Chih Kung; Sheu, Joen Rong; Huang, Chun Jen.

In: Evidence-based Complementary and Alternative Medicine, Vol. 2015, 504061, 2015.

Research output: Contribution to journalArticle

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