Salicylic acid mediates alternative signal transduction pathways for pathogenesis-related acidic β-1,3-glucanase (protein N) induction in tobacco cell suspension culture

Hsien Jung Chen, Wen Chi Hou, Joseph Kuc', Yaw Huei Lin

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Salicylic acid (SA) plays a central role in plant acquired resistance and induces gene expression of a group of pathogenesis-related (PR) proteins. We have previously reported that tobacco cells display Ca2+-dependent and Ca2+-independent excretion modes of SA in suspension culture depending on the applied SA concentrations (Chen et al. 2001). In this study, same concentrations of SA (200 μmol/L and 20 μmol/L) were used to compare their effects on PR-N, an acidic β-1,3-glucanase, gene induction and mRNA accumulation in tobacco cell suspension culture. SA at both concentrations induced PR-N gene expression. The induction by 200 μmol/L SA was significantly inhibited by EGTA (a Ca2+ ion chelator) or reduced glutathione (an active oxygen species scavenger). However, these two compounds have little or no effect on PR-N gene induction by 20 μmol/L SA. Either calcium ionophore (A23187) or catalase inhibitor (3-amino-1,2,4-triazole; 3AT) activated PR-N gene expression. As observed for 200 μmol/L SA, the induction by A23187 and 3AT was also blocked by EGTA and reduced glutathione, respectively. A SA functional analog, 2,6-dichloroisonicotinic acid (INA), induced PR-N gene expression. However, EGTA and reduced glutathione had little or no effect on the induction by 20 μmol/L INA, same as observed for the induction by 20 μmol/L SA. Based on these data, we conclude that SA could mediate alternative signal transduction pathways leading to PR-N gene induction in tobacco cell suspension culture. SA concentration seems to play a key role in the activation of different components of signal transduction pathways. Active oxygen species elevation and external Ca2+ influx are components likely associated with 200 μmol/L SA activation mechanism. Another signal transduction pathway not associated with these two components, however, may be responsible for the effect of SA at 20 μmol/L.

Original languageEnglish
Pages (from-to)331-337
Number of pages7
JournalJournal of Plant Physiology
Volume159
Issue number4
Publication statusPublished - 2002

Fingerprint

Salicylic Acid
salicylic acid
cell suspension culture
Tobacco
signal transduction
Signal Transduction
Suspensions
tobacco
pathogenesis
Cell Culture Techniques
Proteins
proteins
gene induction
Egtazic Acid
calcium
Glutathione
Gene Expression
glutathione
gene expression
Calcimycin

Keywords

  • Calcium
  • Glutathione
  • Pathogenesis-related protein
  • Salicyclic acid
  • Signal

ASJC Scopus subject areas

  • Plant Science

Cite this

Salicylic acid mediates alternative signal transduction pathways for pathogenesis-related acidic β-1,3-glucanase (protein N) induction in tobacco cell suspension culture. / Chen, Hsien Jung; Hou, Wen Chi; Kuc', Joseph; Lin, Yaw Huei.

In: Journal of Plant Physiology, Vol. 159, No. 4, 2002, p. 331-337.

Research output: Contribution to journalArticle

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abstract = "Salicylic acid (SA) plays a central role in plant acquired resistance and induces gene expression of a group of pathogenesis-related (PR) proteins. We have previously reported that tobacco cells display Ca2+-dependent and Ca2+-independent excretion modes of SA in suspension culture depending on the applied SA concentrations (Chen et al. 2001). In this study, same concentrations of SA (200 μmol/L and 20 μmol/L) were used to compare their effects on PR-N, an acidic β-1,3-glucanase, gene induction and mRNA accumulation in tobacco cell suspension culture. SA at both concentrations induced PR-N gene expression. The induction by 200 μmol/L SA was significantly inhibited by EGTA (a Ca2+ ion chelator) or reduced glutathione (an active oxygen species scavenger). However, these two compounds have little or no effect on PR-N gene induction by 20 μmol/L SA. Either calcium ionophore (A23187) or catalase inhibitor (3-amino-1,2,4-triazole; 3AT) activated PR-N gene expression. As observed for 200 μmol/L SA, the induction by A23187 and 3AT was also blocked by EGTA and reduced glutathione, respectively. A SA functional analog, 2,6-dichloroisonicotinic acid (INA), induced PR-N gene expression. However, EGTA and reduced glutathione had little or no effect on the induction by 20 μmol/L INA, same as observed for the induction by 20 μmol/L SA. Based on these data, we conclude that SA could mediate alternative signal transduction pathways leading to PR-N gene induction in tobacco cell suspension culture. SA concentration seems to play a key role in the activation of different components of signal transduction pathways. Active oxygen species elevation and external Ca2+ influx are components likely associated with 200 μmol/L SA activation mechanism. Another signal transduction pathway not associated with these two components, however, may be responsible for the effect of SA at 20 μmol/L.",
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