Sacchachitin, a novel chitin-polysaccharide conjugate macromolecule present in Ganoderma lucidum: Purification, composition, and properties

Chao Ming Chuang, Hui Er Wang, Chi Huang Chang, Chiung Chi Peng, Yaw Bee Ker, Jing Erh Lai, Kuan Chou Chen, Robert Y. Peng

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Context: The extraction method and the crude wound healing effects of sacchachitin from Ganoderma tsugae Murr. (Ganodermataceae) has been cited. However, its purity is still largely limited. Objective: An improvement of the fractionation protocol to purify the sacchachitin from Ganoderma lucidum L. (Ganodermataceae) (SGL) is needed. Methods: Fruiting bodies were extracted with double distilled water and subsequently the residue treated with 95% ethanol and then 40% ethanol. After being filtered, the pH of the supernatant was adjusted to 4.0 with 1 N HCl and lyophilized. The supernatant was added (3:1 v/v) ethanol, the precipitate was collected, 2% NaOH was added and refluxed. The supernatant was collected with pH adjusted to 4.0, then treated with 10% potassium hydroxide (KOH) with repeating acid precipitation and (3:1) ethanol precipitation twice more to obtain the sacchachitin. Results: SGL had a hexosamine content 16.3% (w/w), firmly linked to a talomannan. Its Fourier Transform Infrared Spectroscopy (FTIR) spectrum revealed specific absorption (in cm-1) νO-H 3455.5 b,s, amide νC=O 1678.5, and amide I° δN-H 1550.4. The percentage deacetylation degree was 37.6 and 39.4% for SGL and MSC, respectively. As contrast, MSC contained only 6.6% of hexosamine with a low protein/carbohydrate ratio 0.35 comparing to 0.82 for SGL. SGL was only moderately strong antioxidant regarding the anti-DPPH, antihydroxyl free radical, and antisuperoxide anion capabilities, exhibiting an IC33 values of 10 mg/mL (the highest scavenging capability never exceeding 33%), 0.9 mg/mL, and 4.8 mg/mL, respectively. Conclusion: We have successfully isolated the pure sacchachitin from the fruiting bodies of G. lucidum that exhibits potent antioxidative activity and may be useful in fabrication of the artificial skin composite substitute.

Original languageEnglish
Pages (from-to)84-95
Number of pages12
JournalPharmaceutical Biology
Volume51
Issue number1
DOIs
Publication statusPublished - Jan 2013

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Reishi
Chitin
Polysaccharides
Ethanol
Artificial Skin
Hexosamines
Amides
Ganoderma
Fourier Transform Infrared Spectroscopy
Wound Healing
Free Radicals
Anions
Antioxidants
Carbohydrates
Acids
Water
Proteins

Keywords

  • Antioxidant
  • Artificial skin
  • Biomedical materials
  • IR spectrum
  • Talomannan
  • Wound healing

ASJC Scopus subject areas

  • Drug Discovery
  • Pharmacology
  • Pharmaceutical Science
  • Complementary and alternative medicine
  • Molecular Medicine

Cite this

Sacchachitin, a novel chitin-polysaccharide conjugate macromolecule present in Ganoderma lucidum : Purification, composition, and properties. / Chuang, Chao Ming; Wang, Hui Er; Chang, Chi Huang; Peng, Chiung Chi; Ker, Yaw Bee; Lai, Jing Erh; Chen, Kuan Chou; Peng, Robert Y.

In: Pharmaceutical Biology, Vol. 51, No. 1, 01.2013, p. 84-95.

Research output: Contribution to journalArticle

Chuang, Chao Ming ; Wang, Hui Er ; Chang, Chi Huang ; Peng, Chiung Chi ; Ker, Yaw Bee ; Lai, Jing Erh ; Chen, Kuan Chou ; Peng, Robert Y. / Sacchachitin, a novel chitin-polysaccharide conjugate macromolecule present in Ganoderma lucidum : Purification, composition, and properties. In: Pharmaceutical Biology. 2013 ; Vol. 51, No. 1. pp. 84-95.
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AU - Peng, Chiung Chi

AU - Ker, Yaw Bee

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AU - Peng, Robert Y.

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N2 - Context: The extraction method and the crude wound healing effects of sacchachitin from Ganoderma tsugae Murr. (Ganodermataceae) has been cited. However, its purity is still largely limited. Objective: An improvement of the fractionation protocol to purify the sacchachitin from Ganoderma lucidum L. (Ganodermataceae) (SGL) is needed. Methods: Fruiting bodies were extracted with double distilled water and subsequently the residue treated with 95% ethanol and then 40% ethanol. After being filtered, the pH of the supernatant was adjusted to 4.0 with 1 N HCl and lyophilized. The supernatant was added (3:1 v/v) ethanol, the precipitate was collected, 2% NaOH was added and refluxed. The supernatant was collected with pH adjusted to 4.0, then treated with 10% potassium hydroxide (KOH) with repeating acid precipitation and (3:1) ethanol precipitation twice more to obtain the sacchachitin. Results: SGL had a hexosamine content 16.3% (w/w), firmly linked to a talomannan. Its Fourier Transform Infrared Spectroscopy (FTIR) spectrum revealed specific absorption (in cm-1) νO-H 3455.5 b,s, amide νC=O 1678.5, and amide I° δN-H 1550.4. The percentage deacetylation degree was 37.6 and 39.4% for SGL and MSC, respectively. As contrast, MSC contained only 6.6% of hexosamine with a low protein/carbohydrate ratio 0.35 comparing to 0.82 for SGL. SGL was only moderately strong antioxidant regarding the anti-DPPH, antihydroxyl free radical, and antisuperoxide anion capabilities, exhibiting an IC33 values of 10 mg/mL (the highest scavenging capability never exceeding 33%), 0.9 mg/mL, and 4.8 mg/mL, respectively. Conclusion: We have successfully isolated the pure sacchachitin from the fruiting bodies of G. lucidum that exhibits potent antioxidative activity and may be useful in fabrication of the artificial skin composite substitute.

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