RPS12 increases the invasiveness in cervical cancer activated by c-Myc and inhibited by the dietary flavonoids luteolin and quercetin

Cheng Wei Lin, Gi Ming Lai, Ku Chung Chen, Tsung Han Lin, Jhen Jia Fan, Rhu Long Hsu, Chih Ming Chou, Chun Mao Lin, Chithan C. Kandaswami, Ming Ting Lee, Chia Hsiung Cheng

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The dietary flavonoids luteolin and quercetin are reported to inhibit cancer mobility; however, the regulatory effect of luteolin and quercetin on RPS12 is still unclear. Here, we found that A431-III cells expressed a higher level of RPS12 than A431-P cells. The flavonoids luteolin and quercetin reduced RPS12 and c-Myc expressions via Akt/mTOR signalling. The Akt inhibitor LY294002 and mTOR inhibitor rapamycin reduced RPS12 and c-Myc expressions. The c-Myc inhibitor 10058-F4 reduced RPS12 expression and promoter transactivation. The overexpression of c-Myc increased RPS12 expression. Akt, mTOR, and c-Myc inhibitor blocked cell migration. Reducing RPS12 expression via 10058-F4 and shRNAs reduced cell invasion. This study reveals that RPS12 is upregulated via Akt/mTOR/c-Myc signalling and increased cell mobility. Luteolin and quercetin blocked Akt/mTOR/c-Myc signalling to reduce RPS12 level and downstream cell mobility. These data suggest a possible role of RPS12 in cell mobility and may be a potential therapy target for cervical cancer.

Original languageEnglish
Pages (from-to)236-247
Number of pages12
JournalJournal of Functional Foods
Volume19
DOIs
Publication statusPublished - Dec 1 2015

Keywords

  • 10058-F4
  • Agarose
  • C-Myc
  • Cervical cancer
  • DMSO
  • Invasiveness
  • Luteolin
  • LY294002
  • MTT
  • Quercetin
  • Rapamycin
  • RPS12

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)
  • Nutrition and Dietetics

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