Rous-associated virus 1-induced erythroleukemic cells exhibit a weakly transformed phenotype in vitro and release c-erbB-containing retroviruses unable to transform fibroblasts

H. Beug, M. J. Hayman, M. B. Raines, H. J. Kung, B. Vennström

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Avian leukosis viruses induce erythroblastosis in chicks by integrating into the c-erbB gene and thus activating c-erbB transcription. We characterized Rous-associated virus 1-induced leukemic erythroblasts in vitro and showed that they mostly resemble erythropoietin-independent but otherwise normal erythroid progenitors. Some leukemic cells, however, were able to both differentiate and proliferate extensively in vitro. All 14 leukemias studies expressed high levels of erbB-related proteins that were 5 to 10 kilodaltons larger but otherwise very similar to the gp74(erbB) protein of avian erythroblastosis virus ES4 with respect to biosynthesis, glycosylation, and cell surface expression. Two leukemias contained and released retroviruses that transduced erbB. Chicken embryo fibroblasts fully infected with these viruses expressed high levels of erbB RNA and protein but retained a normal phenotype. Our results suggest that certain forms of c-erbB, activated by long terminal repeat insertion or viral transduction, are capable of inducing erythroleukemia but unable to transform fibroblasts.

Original languageEnglish
Pages (from-to)1127-1138
Number of pages12
JournalJournal of Virology
Volume57
Issue number3
Publication statusPublished - Jul 2 1986
Externally publishedYes

Fingerprint

Retroviridae
Avian Leukosis Virus
fibroblasts
Fibroblasts
leukemia
Phenotype
phenotype
Avian erythroblastosis virus
viruses
erbB Genes
Leukemia
Alpharetrovirus
erythroblasts
Avian leukosis virus
erythropoietin
Erythroblasts
Leukemia, Erythroblastic, Acute
Proteins
terminal repeat sequences
proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Rous-associated virus 1-induced erythroleukemic cells exhibit a weakly transformed phenotype in vitro and release c-erbB-containing retroviruses unable to transform fibroblasts. / Beug, H.; Hayman, M. J.; Raines, M. B.; Kung, H. J.; Vennström, B.

In: Journal of Virology, Vol. 57, No. 3, 02.07.1986, p. 1127-1138.

Research output: Contribution to journalArticle

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AB - Avian leukosis viruses induce erythroblastosis in chicks by integrating into the c-erbB gene and thus activating c-erbB transcription. We characterized Rous-associated virus 1-induced leukemic erythroblasts in vitro and showed that they mostly resemble erythropoietin-independent but otherwise normal erythroid progenitors. Some leukemic cells, however, were able to both differentiate and proliferate extensively in vitro. All 14 leukemias studies expressed high levels of erbB-related proteins that were 5 to 10 kilodaltons larger but otherwise very similar to the gp74(erbB) protein of avian erythroblastosis virus ES4 with respect to biosynthesis, glycosylation, and cell surface expression. Two leukemias contained and released retroviruses that transduced erbB. Chicken embryo fibroblasts fully infected with these viruses expressed high levels of erbB RNA and protein but retained a normal phenotype. Our results suggest that certain forms of c-erbB, activated by long terminal repeat insertion or viral transduction, are capable of inducing erythroleukemia but unable to transform fibroblasts.

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