Roles of testosterone and amygdaloid LTP induction in determining sex differences in fear memory magnitude

Li Shen Chen, Wen Yu Tzeng, Jia Ying Chuang, Chianfang G. Cherng, Po Wu Gean, Lung Yu

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Women are thought to form fear memory more robust than men do and testosterone is suspected to play a role in determining such a sex difference. Mouse cued fear freezing was used to study the sex-related susceptibility and the role of testosterone in fear memory in humans. A 75-dB tone was found to provoke weak freezing, while 0.15-mA and 0.20-mA footshock caused strong freezing responses. No sex differences were noticed in the tone- or footshock-induced (naïve fear) freezing. Following the conditionings, female mice exhibited greater tone (cued fear)-induced freezing than did male mice. Nonetheless, female mice demonstrated indistinctive cued fear freezing across the estrous phases and ovariectomy did not affect such freezing in female mice. Orchidectomy enhanced the cued fear freezing in male mice. Systemic testosterone administrations and an intra-lateral nucleus of amygdala (LA) testosterone infusion diminished the cued fear freezing in orchidectomized male mice, while pretreatment with flutamide (Flu) eradicated these effects. Long-term potentiation (LTP) magnitude in LA has been known to correlate with the strength of the cued fear conditioning. We found that LA LTP magnitude was indeed greater in female than male mice. Orchidectomy enhanced LTP magnitude in males' LA, while ovariectomy decreased LTP magnitude in females' LA. Testosterone decreased LTP magnitude in orchidectomized males' LA and estradiol enhanced LTP magnitude in ovariectomized females' LA. Finally, male mice had lower LA GluR1 expression than female mice and orchidectomy enhanced the GluR1 expression in male mice. These findings, taken together, suggest that testosterone plays a critical role in rendering the sex differences in the cued fear freezing and LA LTP. Testosterone is negatively associated with LA LTP and the cued fear memory in male mice. However, ovarian hormones and LA LTP are loosely associated with the cued fear memory in female mice.

Original languageEnglish
Pages (from-to)498-508
Number of pages11
JournalHormones and Behavior
Volume66
Issue number3
DOIs
Publication statusPublished - 2014
Externally publishedYes

Fingerprint

Long-Term Potentiation
Sex Characteristics
Fear
Testosterone
Freezing
Orchiectomy
Ovariectomy
Basolateral Nuclear Complex
Flutamide
Estradiol

Keywords

  • Estrous cycle
  • Fear memory
  • Mice
  • Mood disorder
  • Sex difference
  • Testosterone

ASJC Scopus subject areas

  • Endocrinology
  • Behavioral Neuroscience
  • Endocrine and Autonomic Systems

Cite this

Roles of testosterone and amygdaloid LTP induction in determining sex differences in fear memory magnitude. / Chen, Li Shen; Tzeng, Wen Yu; Chuang, Jia Ying; Cherng, Chianfang G.; Gean, Po Wu; Yu, Lung.

In: Hormones and Behavior, Vol. 66, No. 3, 2014, p. 498-508.

Research output: Contribution to journalArticle

Chen, Li Shen ; Tzeng, Wen Yu ; Chuang, Jia Ying ; Cherng, Chianfang G. ; Gean, Po Wu ; Yu, Lung. / Roles of testosterone and amygdaloid LTP induction in determining sex differences in fear memory magnitude. In: Hormones and Behavior. 2014 ; Vol. 66, No. 3. pp. 498-508.
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