Role of yqiC in the pathogenicity of salmonella and innate immune responses of human intestinal epithelium

Ke Chuan Wang, Chih Hung Huang, Shih Min Ding, Ching Kuo Chen, Hsu Wei Fang, Ming Te Huang, Shiuh Bin Fang

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The yqiC gene of Salmonella enterica serovar Typhimurium (S. Typhimurium) regulates bacterial growth at different temperatures and mice survival after infection. However, the role of yqiC in bacterial colonization and host immunity remains unknown. We infected human LS174T, Caco-2, HeLa, and THP-1 cells with S. Typhimurium wild-type SL1344, its yqiC mutant, and its complemented strain. Bacterial colonization and internalization in the four cell lines significantly reduced on yqiC depletion. Post-infection production of interleukin-8 and human β-defensin-3 in LS174T cells significantly reduced because of yqiC deleted in S. Typhimurium. The phenotype of yqiC mutant exhibited few and short flagella, fimbriae on the cell surface, enhanced biofilm formation, upregulated type-1 fimbriae expression, and reduced bacterial motility. Type-1 fimbriae, flagella, SPI-1, and SPI-2 gene expression was quantified using real-time PCR. The data show that deletion of yqiC upregulated fimA and fimZ expression and downregulated flhD, fliZ, invA, and sseB expression. Furthermore, thin-layer chromatography and high-performance liquid chromatography revealed the absence of menaquinone in the yqiC mutant, thus validating the importance of yqiC in the bacterial electron transport chain. Therefore, YqiC can negatively regulate FimZ for type-1 fimbriae expression and manipulate the functions of its downstream virulence factors including flagella, SPI-1, and SPI-2 effectors.

Original languageEnglish
Article number1614
JournalFrontiers in Microbiology
Volume7
Issue numberOCT
DOIs
Publication statusPublished - Oct 10 2016

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Flagella
Intestinal Mucosa
Innate Immunity
Salmonella
Virulence
Defensins
Vitamin K 2
Salmonella enterica
Virulence Factors
Biofilms
Thin Layer Chromatography
Electron Transport
Infection
Interleukin-8
Real-Time Polymerase Chain Reaction
Immunity
Down-Regulation
High Pressure Liquid Chromatography
Phenotype
Gene Expression

Keywords

  • Bacterial colonization
  • Flagella
  • Human β-defensin-3
  • Interleukin-8
  • Menaquinone
  • Salmonella Typhimurium
  • Type-1 fimbriae
  • yqiC

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Role of yqiC in the pathogenicity of salmonella and innate immune responses of human intestinal epithelium. / Wang, Ke Chuan; Huang, Chih Hung; Ding, Shih Min; Chen, Ching Kuo; Fang, Hsu Wei; Huang, Ming Te; Fang, Shiuh Bin.

In: Frontiers in Microbiology, Vol. 7, No. OCT, 1614, 10.10.2016.

Research output: Contribution to journalArticle

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abstract = "The yqiC gene of Salmonella enterica serovar Typhimurium (S. Typhimurium) regulates bacterial growth at different temperatures and mice survival after infection. However, the role of yqiC in bacterial colonization and host immunity remains unknown. We infected human LS174T, Caco-2, HeLa, and THP-1 cells with S. Typhimurium wild-type SL1344, its yqiC mutant, and its complemented strain. Bacterial colonization and internalization in the four cell lines significantly reduced on yqiC depletion. Post-infection production of interleukin-8 and human β-defensin-3 in LS174T cells significantly reduced because of yqiC deleted in S. Typhimurium. The phenotype of yqiC mutant exhibited few and short flagella, fimbriae on the cell surface, enhanced biofilm formation, upregulated type-1 fimbriae expression, and reduced bacterial motility. Type-1 fimbriae, flagella, SPI-1, and SPI-2 gene expression was quantified using real-time PCR. The data show that deletion of yqiC upregulated fimA and fimZ expression and downregulated flhD, fliZ, invA, and sseB expression. Furthermore, thin-layer chromatography and high-performance liquid chromatography revealed the absence of menaquinone in the yqiC mutant, thus validating the importance of yqiC in the bacterial electron transport chain. Therefore, YqiC can negatively regulate FimZ for type-1 fimbriae expression and manipulate the functions of its downstream virulence factors including flagella, SPI-1, and SPI-2 effectors.",
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AU - Fang, Hsu Wei

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