Role of transcriptional factors Sp1, c-Rel, and c-Jun in LPS-induced C/EBPd gene expression of mouse macrophages

Y. W. Liu, C. C. Chen, J. M. Wang, W. C. Chang, Y. C. Huang, S. Y. Chung, B. K. Chen, J. J. Hung

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Transcription factor C/EBPs are involved in the regulation of various cellular responses. Here, it was suggested that C/EBPd gene was activated by lipopolysaccharide (LPS) through transcription factors Sp1, c-Rel, and c-Jun. Assay of the luciferase reporter vectors containing a 5'-deletion of the C/ EBPd gene promoter indicated that a LPS-responsive element was positioned between - 345 and - 35 bp of mouse C/EBPd gene promoter. Transcription factors Sp1, c-Rel, and c-Jun bound to this region were identified using both in vivo chromatin immunoprecipitation and in vitro DNA-protein binding assays. LPS enhanced the proteins and DNA binding capacities of c-Rel and c-Jun, and the downstream Sp1 site was essential for LPS-induced C/EBPd gene. Treatment of cells with ERK/JNK/p38 inhibitors or NF-kB inhibitor inhibited the LPS-induced C/EBPd gene expression by inhibiting c-Jun, c-Rel, and p300 binding to DNA. Our findings provide a better understanding of LPS-induced C/EBPd gene expression.

Original languageEnglish
Pages (from-to)3282-3294
Number of pages13
JournalCellular and Molecular Life Sciences
Volume64
Issue number24
DOIs
Publication statusPublished - Dec 2007
Externally publishedYes

Keywords

  • C-Jun
  • C-Rel
  • LPS
  • MAPK
  • Monocytes/macrophages
  • NF-kB
  • Sp1
  • Transcription factors C/EBPd

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Molecular Medicine
  • Pharmacology
  • Cellular and Molecular Neuroscience
  • Biochemistry, Genetics and Molecular Biology(all)

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