Role of SUMO-Interacting Motif in Daxx SUMO Modification, Subnuclear Localization, and Repression of Sumoylated Transcription Factors

Ding Yen Lin, Yen Sung Huang, Jen Chong Jeng, Hong Yi Kuo, Che Chang Chang, Ting Ting Chao, Chun Chen Ho, Yun Ching Chen, Tong Ping Lin, Hsin I. Fang, Chih Chang Hung, Ching Shu Suen, Ming Jing Hwang, Kun Sang Chang, Gerd G. Maul, Hsiu Ming Shih

Research output: Contribution to journalArticle

290 Citations (Scopus)

Abstract

Small ubiquitin-like modifier (SUMO) modification has emerged as an important posttranslational control of protein functions. Daxx, a transcriptional corepressor, was reported to repress the transcriptional potential of several transcription factors and target to PML oncogenic domains (PODs) via SUMO-dependent interactions. The mechanism by which Daxx binds to sumoylated factors mediating transcriptional and subnuclear compartmental regulation remains unclear. Here, we define a SUMO-interacting motif (SIM) within Daxx and show it to be crucial for targeting Daxx to PODs and for transrepression of several sumoylated transcription factors, including glucocorticoid receptor (GR). In addition, the capability of Daxx SIM to bind SUMO also controls Daxx sumoylation. We further demonstrate that arsenic trioxide-induced sumoylation of PML correlates with a change of endogenous Daxx partitioning from GR-regulated gene promoter to PODs and a relief of Daxx repression on GR target gene expression. Our results provide mechanistic insights into Daxx in SUMO-dependent transcriptional control and subnuclear compartmentalization.

Original languageEnglish
Pages (from-to)341-354
Number of pages14
JournalMolecular Cell
Volume24
Issue number3
DOIs
Publication statusPublished - Nov 3 2006
Externally publishedYes

Fingerprint

Ubiquitin
Transcription Factors
Glucocorticoid Receptors
Sumoylation
Co-Repressor Proteins
Gene Expression
Genes
Proteins

Keywords

  • CELLBIO
  • PROTEINS

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Role of SUMO-Interacting Motif in Daxx SUMO Modification, Subnuclear Localization, and Repression of Sumoylated Transcription Factors. / Lin, Ding Yen; Huang, Yen Sung; Jeng, Jen Chong; Kuo, Hong Yi; Chang, Che Chang; Chao, Ting Ting; Ho, Chun Chen; Chen, Yun Ching; Lin, Tong Ping; Fang, Hsin I.; Hung, Chih Chang; Suen, Ching Shu; Hwang, Ming Jing; Chang, Kun Sang; Maul, Gerd G.; Shih, Hsiu Ming.

In: Molecular Cell, Vol. 24, No. 3, 03.11.2006, p. 341-354.

Research output: Contribution to journalArticle

Lin, DY, Huang, YS, Jeng, JC, Kuo, HY, Chang, CC, Chao, TT, Ho, CC, Chen, YC, Lin, TP, Fang, HI, Hung, CC, Suen, CS, Hwang, MJ, Chang, KS, Maul, GG & Shih, HM 2006, 'Role of SUMO-Interacting Motif in Daxx SUMO Modification, Subnuclear Localization, and Repression of Sumoylated Transcription Factors', Molecular Cell, vol. 24, no. 3, pp. 341-354. https://doi.org/10.1016/j.molcel.2006.10.019
Lin, Ding Yen ; Huang, Yen Sung ; Jeng, Jen Chong ; Kuo, Hong Yi ; Chang, Che Chang ; Chao, Ting Ting ; Ho, Chun Chen ; Chen, Yun Ching ; Lin, Tong Ping ; Fang, Hsin I. ; Hung, Chih Chang ; Suen, Ching Shu ; Hwang, Ming Jing ; Chang, Kun Sang ; Maul, Gerd G. ; Shih, Hsiu Ming. / Role of SUMO-Interacting Motif in Daxx SUMO Modification, Subnuclear Localization, and Repression of Sumoylated Transcription Factors. In: Molecular Cell. 2006 ; Vol. 24, No. 3. pp. 341-354.
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