Role of pituitary adenylate cyclase-activating polypeptide (PACAP) in the action of ginsenoside Rh2 against beta-amyloid-induced inhibition of rat brain astrocytes

Po Chuen Shieh, Chiung Wen Tsao, Jin Shiou Li, Hung Tsung Wu, Ya Ju Wen, Dai Huang Kou, Juei Tang Cheng

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Ginsenoside, the active principles in Panax ginseng root, has been demonstrated to show neurotrophic and neuroprotective actions for prevention of neuron degeneration. Deposition of β-amyloid peptide (Aβ) causes neurotoxicity through the formation of plaques in brains with Alzheimer's disease. Pituitary adenylate cyclase-activating polypeptide (PACAP) is introduced as a neurotrophic factor to promote cell survival. However, effect of Rh2, one of ginsenosides, on PACAP expression induced by Aβ remains unclear. In the present study, we found that Rh2 stimulates PACAP gene expression and cell proliferation in type I rat brain astrocytes (RBA1) cells and both effects were not modified by the estrogen antagonists (MPP or ICI 182780). Also, Rh2 ameliorates the RBA1 growth inhibition of Aβ. Moreover, blockade of PACAP receptor PAC1 using PACAP (6-38) inhibits all the actions of Rh2. These results suggest that Rh2 can induce an increase of PACAP to activate PAC1, but not estrogen receptor, and thereby leads to attenuate Aβ-induced toxicity. Thus, ginseng seems useful in the prevention of dementia.

Original languageEnglish
Pages (from-to)1-5
Number of pages5
JournalNeuroscience Letters
Volume434
Issue number1
DOIs
Publication statusPublished - Mar 21 2008
Externally publishedYes

Keywords

  • β-Amyloid
  • Ginsenoside Rh2
  • Pituitary adenylate cyclase-activating polypeptide (PACAP)
  • Rat brain astrocytes (RBA1)

ASJC Scopus subject areas

  • Neuroscience(all)

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