Role of pirenoxine in the effects of catalin on in vitro ultravioletinduced lens protein turbidity and selenite-induced cataractogenesis in vivo

Chao Chien Hu, Jiahn Haur Liao, Kuang Yang Hsu, I. Lin Lin, Ming Hsuan Tsai, Wen Hsin Wu, Tzu Tang Wei, Yi Shiang Huang, Shih Jiuan Chiu, Hsiang Yin Chen, Shih Hsiung Wu, Tzu Hua Wu

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Purpose: In this study, we investigated the biochemical pharmacology of pirenoxine (PRX) and catalin under in vitro selenite/calcium- and ultraviolet (UV)-induced lens protein turbidity challenges. The systemic effects of catalin were determined using a selenite-induced cataractogenesis rat model. Methods: In vitro cataractogenesis assay systems (including UVB/C photo-oxidation of lens crystallins, calpain-induced proteolysis, and selenite/calcium-induced turbidity of lens crystallin solutions) were used to screen the activity of PRX and catalin eye drop solutions. Turbidity was identified as the optical density measured using spectroscopy at 405 nm. We also determined the in vivo effects of catalin on cataract severity in a selenite-induced cataract rat model. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was applied to analyze the integrity of crystallin samples. Results: PRX at 1,000 μM significantly delayed UVC-induced turbidity formation compared to controls after 4 h of UVC exposure (p

Original languageEnglish
Pages (from-to)1862-1870
Number of pages9
JournalMolecular Vision
Volume17
Publication statusPublished - 2011

    Fingerprint

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Hu, C. C., Liao, J. H., Hsu, K. Y., Lin, I. L., Tsai, M. H., Wu, W. H., Wei, T. T., Huang, Y. S., Chiu, S. J., Chen, H. Y., Wu, S. H., & Wu, T. H. (2011). Role of pirenoxine in the effects of catalin on in vitro ultravioletinduced lens protein turbidity and selenite-induced cataractogenesis in vivo. Molecular Vision, 17, 1862-1870.