Role of hyperglycaemia in the pathogenesis of hypotension observed in type-1 diabetic rats

I. Min Liu, Cheng Kuei Chang, Shiow Wen Juang, Dai Huang Kou, Yat Ching Tong, Kai Chun Cheng, Juei Tang Cheng

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The role of hyperglycaemia in the pathogenesis of hypotension in diabetic disorders was investigated using the changes in cardiac M 2-muscarinic receptor (M 2-mAChR) gene expression in type-1-like diabetic rats and cultured cardiomyocytes. Blood pressure was markedly decreased in diabetic rats following the intravenous injection of streptozotocin (STZ) for 8 weeks. Also, the baroreflex sensitivity (ΔHR/ΔBP), as measured by the changes in heart rate (ΔHR) and mean blood pressure (ΔBP) 1 min after the intravenous injection of phenylephrine (10 μg/kg), was significantly increased. Arecaidine propargyl ester (APE), a M 2-mAChR agonist produced a marked reduction in heart rate in these diabetic rats. Normalization of plasma glucose in diabetic rats using insulin (0.5 IU) or phlorizin (1 mg/kg) injection attenuated the blood pressure reduction and reversed the mRNA and protein levels of cardiac M 2-mAChR. A high concentration of glucose (20 mmol/l) directly influenced the increase in gene expression of M 2-mAChR in the H9c2 cardiac cell line. Hyperglycaemia induced an increase in cardiac M 2-mAChR gene expression, suggesting a role in the pathogenesis of hypotension in diabetic disorders.

Original languageEnglish
Pages (from-to)292-300
Number of pages9
JournalInternational Journal of Experimental Pathology
Volume89
Issue number4
DOIs
Publication statusPublished - Aug 2008

Keywords

  • Cardiac M -muscarinic receptor
  • Diabetic rats
  • Gene expression
  • Hyperglycaemia
  • Insulin
  • Phlorizin

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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