Role of glycogen synthase kinase3β/GATA-4 in tumor necrosis factor-α-regulated sarcoplasmic recticulum Ca2+-ATPase expressions in cardiomyocytes

Nan Hung Pan, Yao Chang Chen, Yu Hsun Kao, Yi Jen Chen

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: Sarcoplasmic reticulum Ca2+-ATPaSe (SERCA2a) plays an essential role in Ca2+ homeostasis and cardiac functions. Tumor necrosis factor-α (TNF-α) decreases the SERCA2a, which can induce cardiac dysfunction. GATA-4 is a critical transcription factor in load-mediated SERCA2a expression and negatively regulated by glycogen synthase kinase3β (GSK3β). The present study was to evaluate whether TNF-α can modulate SERCA2a via GSK3β and GATA-4. Methods: To determine whether TNF-α could modulate the expression of SERCA2a, GSK3β, and GATA-4, HL-1 cells were treated with TNF-α (50 ng/ml) for 24 hours. The expressions of SERCA2a, GSK3β and nuclear GATA-4 were measured by real-time RT-PCR and immunoblot analysis. Results: TNF-α decreased the RNA expression of SERCA2a by 30 ± 8%. In contrast, the protein level of GSK3β was not significantly changed by TNF-α. In addition, TNF-α did not alter the protein expression of nuclear GATA-4. Conclusion: TNF-α decreases SERCA2a expression. These findings suggest that GSK3β and GATA-4 may not play an important role in SERCA2a modulation during acute inflammatory stage.

Original languageEnglish
Pages (from-to)179-183
Number of pages5
JournalActa Cardiologica Sinica
Volume26
Issue number3
Publication statusPublished - Sep 2010

Keywords

  • G3k3β
  • GATA-4
  • Heart failure
  • SERCA2a
  • Tumor necrosis factor-α

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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