Role of DNA methyltransferase 1 in pharyngeal cancer related to treatment resistance

Chih Cheng Chen, Wen Cheng Chen, Wen Hung Wang, Chang Hsien Lu, Paul Yang Lin, Kuan Der Lee, Miao Fen Chen

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background: The purpose of the present study was to highlight the role of DNA methyltransferase 1 (DNMT1) and its potential for improving treatment and informing prognosis for pharyngeal cancer. Methods: Human pharyngeal cancer cell lines FaDu and its derivative FaDu-C225-R were selected for cellular experiments. Furthermore, 95 pharyngeal cancer tissue specimens were analyzed by immunohistochemical staining. Results: DNMT1 was over-expressed in pharyngeal cancer specimens and cells with activated interleukin (IL)-6 signaling. When DNMT1 activity was blocked, accelerated tumor growth and treatment resistance were overcome as demonstrated by cell culture and animal experiments. The reduction in DNMT1 was associated with increased apoptosis, cell cycle arrest, and augmented irradiation-induced free radical levels and DNA damage. Furthermore, positive staining for DNMT1 in clinical cancer specimens was significantly linked to lower rates of response to treatments and shorter survival of patients with pharyngeal cancer. Conclusion: DNMT1 may be a significant clinical predictor and a potential treatment strategy against head and neck cancer.

Original languageEnglish
Pages (from-to)1132-1143
Number of pages12
JournalHead and Neck
Volume33
Issue number8
DOIs
Publication statusPublished - Aug 2011
Externally publishedYes

Keywords

  • DNMT1
  • IL-6
  • pharyngeal cancer

ASJC Scopus subject areas

  • Otorhinolaryngology

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  • Cite this

    Chen, C. C., Chen, W. C., Wang, W. H., Lu, C. H., Lin, P. Y., Lee, K. D., & Chen, M. F. (2011). Role of DNA methyltransferase 1 in pharyngeal cancer related to treatment resistance. Head and Neck, 33(8), 1132-1143. https://doi.org/10.1002/hed.21586