RNA-binding motif Protein 4 translocates to cytoplasmic granules and suppresses translation via argonaute2 duringmuscle cell differentiation

Jung Chun Lin, Woan Yuh Tarn

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The RNA-binding motif protein 4 (RBM4) plays multiple roles in mRNA metabolism, including translation control. RBM4 suppresses cap-dependent translation but activates internal ribosome entry site-mediated translation. Because of its high expression level in muscle and heart, we investigated the function of RBM4 in myoblast cells. Here, we demonstrate that RBM4 is phosphorylated and translocates to the cytoplasm in mouse C2C12 cells upon cell differentiation. Notably, RBM4 is transiently deposited into cytoplasmic granules containing microtubule assembly factors as well as poly(A)+ RNAs. Moreover, RBM4 colocalizes with the components of micro-ribonucleoproteins, including the Argonaute2 (Ago2) protein, during muscle cell differentiation. RBM4 interacts directly with Ago2 and may recruit Ago2 to suppress translation of target mRNAs. Furthermore, RBM4 selectively associates with muscle cell-specific microRNAs and potentiates their translation repression activity by promoting micro-ribonucleoprotein association with target mRNAs. Altogether, our results suggest that RBM4 translocates to the cytoplasm and participates in translation suppression during muscle cell differentiation.

Original languageEnglish
Pages (from-to)34658-34665
Number of pages8
JournalJournal of Biological Chemistry
Volume284
Issue number50
DOIs
Publication statusPublished - Dec 11 2009
Externally publishedYes

Fingerprint

Cytoplasmic Granules
RNA-Binding Proteins
Cell Differentiation
RNA
Muscle
Muscle Cells
Messenger RNA
Ribonucleoproteins
Cells
Cytoplasm
RNA-Binding Motifs
Carrier Proteins
Myoblasts
Protein Biosynthesis
MicroRNAs
Metabolism
Microtubules
Myocardium

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

RNA-binding motif Protein 4 translocates to cytoplasmic granules and suppresses translation via argonaute2 duringmuscle cell differentiation. / Lin, Jung Chun; Tarn, Woan Yuh.

In: Journal of Biological Chemistry, Vol. 284, No. 50, 11.12.2009, p. 34658-34665.

Research output: Contribution to journalArticle

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