Risk assessment of excess drug and sunscreen absorption via skin with ablative fractional laser resurfacing: Optimization of the applied dose for postoperative care

Wei Yu Chen, Chia Lang Fang, Saleh A. Al-Suwayeh, Hung Hsu Yang, Yi Ching Li, Jia You Fang

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The ablative fractional laser is a new modality used for surgical resurfacing. It is expected that laser treatment can generally deliver drugs into and across the skin, which is toxicologically relevant. The aim of this study was to establish skin absorption characteristics of antibiotics, sunscreens, and macromolecules via laser-treated skin and during postoperative periods. Nude mice were employed as the animal model. The skin received a single irradiation of a fractional CO2 laser, using fluences of 4-10 mJ with spot densities of 100-400 spots/cm2. In vitro skin permeation using Franz cells was performed. Levels of skin water loss and erythema were evaluated, and histological examinations with staining by hematoxylin and eosin, cyclooxygenase-2, and claudin-1 were carried out. Significant signs of erythema, edema, and scaling of the skin treated with the fractional laser were evident. Inflammatory infiltration and a reduction in tight junctions were also observed. Laser treatment at 6 mJ increased tetracycline and tretinoin fluxes by 70- and 9-fold, respectively. A higher fluence resulted in a greater tetracycline flux, but lower skin deposition. On the other hand, tretinoin skin deposition increased following an increase in the laser fluence. The fractional laser exhibited a negligible effect on modulating oxybenzone absorption. Dextrans with molecular weights of 4 and 10 kDa showed increased fluxes from 0.05 to 11.05 and 38.54 μg/cm2/h, respectively. The optimized drug dose for skin treated with the fractional laser was 1/70-1/60 of the regular dose. The skin histology and drug absorption had recovered to a normal status within 2-3 days. Our findings provide the first report on risk assessment of excessive skin absorption after fractional laser resurfacing.

Original languageEnglish
Pages (from-to)1363-1374
Number of pages12
JournalLasers in Medical Science
Volume28
Issue number5
DOIs
Publication statusPublished - Sep 2013

Fingerprint

Skin Absorption
Sunscreening Agents
Postoperative Care
Lasers
Skin
Pharmaceutical Preparations
Erythema
Tretinoin
Tetracycline
Claudin-1
Gas Lasers
Tight Junctions
Cyclooxygenase 2
Hematoxylin
Eosine Yellowish-(YS)
Dextrans
Postoperative Period
Nude Mice
Edema
Histology

Keywords

  • Antibiotic
  • Fractional laser
  • Risk assessment
  • Skin absorption
  • Sunscreen

ASJC Scopus subject areas

  • Surgery
  • Dermatology

Cite this

Risk assessment of excess drug and sunscreen absorption via skin with ablative fractional laser resurfacing : Optimization of the applied dose for postoperative care. / Chen, Wei Yu; Fang, Chia Lang; Al-Suwayeh, Saleh A.; Yang, Hung Hsu; Li, Yi Ching; Fang, Jia You.

In: Lasers in Medical Science, Vol. 28, No. 5, 09.2013, p. 1363-1374.

Research output: Contribution to journalArticle

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abstract = "The ablative fractional laser is a new modality used for surgical resurfacing. It is expected that laser treatment can generally deliver drugs into and across the skin, which is toxicologically relevant. The aim of this study was to establish skin absorption characteristics of antibiotics, sunscreens, and macromolecules via laser-treated skin and during postoperative periods. Nude mice were employed as the animal model. The skin received a single irradiation of a fractional CO2 laser, using fluences of 4-10 mJ with spot densities of 100-400 spots/cm2. In vitro skin permeation using Franz cells was performed. Levels of skin water loss and erythema were evaluated, and histological examinations with staining by hematoxylin and eosin, cyclooxygenase-2, and claudin-1 were carried out. Significant signs of erythema, edema, and scaling of the skin treated with the fractional laser were evident. Inflammatory infiltration and a reduction in tight junctions were also observed. Laser treatment at 6 mJ increased tetracycline and tretinoin fluxes by 70- and 9-fold, respectively. A higher fluence resulted in a greater tetracycline flux, but lower skin deposition. On the other hand, tretinoin skin deposition increased following an increase in the laser fluence. The fractional laser exhibited a negligible effect on modulating oxybenzone absorption. Dextrans with molecular weights of 4 and 10 kDa showed increased fluxes from 0.05 to 11.05 and 38.54 μg/cm2/h, respectively. The optimized drug dose for skin treated with the fractional laser was 1/70-1/60 of the regular dose. The skin histology and drug absorption had recovered to a normal status within 2-3 days. Our findings provide the first report on risk assessment of excessive skin absorption after fractional laser resurfacing.",
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